Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tungstate | complete inhibition in vivo | Proteus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur cluster | - |
Proteus vulgaris | |
molybdate | strong induction | Proteus vulgaris | |
Molybdenum | 4-12 atoms per enzyme molecule | Proteus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
750000 | - |
- |
Proteus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus vulgaris | - |
molybdate-inducible | - |
Purification (Comment) | Organism |
---|---|
60fold | Proteus vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
810 | - |
crude cell extract | Proteus vulgaris |
Storage Stability | Organism |
---|---|
-16°C, N2-atmosphere, wet packed cells, 450 days, more than 90% remaining activity | Proteus vulgaris |
room temperature, freeze dried cells, 200 days, more than 80% remaining activity | Proteus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-hydroxy-4-methylpentanoate + 2-oxo-butyrate | - |
Proteus vulgaris | 2-oxo-4-methylpentanoate + (2R)-hydroxybutyrate | - |
r | |
2-hydroxyglutarate + oxidized acceptor | - |
Proteus vulgaris | 2-oxoglutarate + reduced acceptor | - |
r | |
2-oxo-3,5-dienoates + H2 | - |
Proteus vulgaris | ? | - |
r | |
2-oxo-3-(3,4-dihydroxyphenyl)-propionate + H2 | - |
Proteus vulgaris | rosmarinic acid | - |
r | |
2-oxo-3-enoates + H2 | - |
Proteus vulgaris | ? | - |
r | |
lactate + oxidized acceptor | - |
Proteus vulgaris | pyruvate + reduced acceptor | - |
r | |
mandelate + oxidized acceptor | - |
Proteus vulgaris | oxo(phenyl)acetic acid + reduced acceptor | - |
r | |
pantolactone + oxidized acceptor | - |
Proteus vulgaris | dehydropantolactone + reduced acceptor | - |
r | |
phenylpyruvate + H2 | - |
Proteus vulgaris | (2R)-phenyllactate | - |
r | |
phenylpyruvate + reduced benzyl viologen | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized benzyl viologen | - |
r | |
phenylpyruvate + reduced carbamoylmethyl viologen | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized carbamoylmethyl viologen | - |
r | |
phenylpyruvate + reduced cobalt sepulchrate | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized cobalt sepulchrate | - |
r | |
phenylpyruvate + reduced indigocarmine | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized indigocarmine | - |
r | |
phenylpyruvate + reduced kresyl violet | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized kresyl violet | - |
r | |
phenylpyruvate + reduced meldolablue | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized meldolablue | - |
r | |
phenylpyruvate + reduced methylene blue | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized methylene blue | - |
r | |
phenylpyruvate + reduced phenosafranine | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized phenosafranine | - |
r | |
phenylpyruvate + reduced safranine TH | - |
Proteus vulgaris | (2R)-phenyllactate + oxidized safranine TH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
HVOR | - |
Proteus vulgaris |
hydroxycarboxlate-oxidoreductase | - |
Proteus vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Proteus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Proteus vulgaris |