BRENDA - Enzyme Database
show all sequences of 1.1.99.30

Proteus mirabilis dehydrogenates aldonates and aldarates with an (R)-configured alpha-carbon atom to the corresponding 2-oxocarboxylates

Schinschel, C.; Simon, H.; Bioorg. Med. Chem. 2, 483-491 (1994)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
carboxamido-methyl viologen
pH 8.5, 38°C
Proteus mirabilis
0.042
-
benzyl viologen
pH 8.5, 38°C
Proteus mirabilis
0.19
-
oxidized antraquinone-2,6-disulfonate
pH 8.5, 38°C
Proteus mirabilis
0.3
-
D-ribonate
pH 8.5, 38°C
Proteus mirabilis
0.42
-
galactarate
pH 8.5, 38°C
Proteus mirabilis
0.48
-
D-galactonate
pH 8.5, 38°C
Proteus mirabilis
0.64
-
6-phospho-D-gluconate
pH 8.5, 38°C
Proteus mirabilis
0.64
-
D-gulonate
pH 8.5, 38°C
Proteus mirabilis
0.66
-
Lactobionate
pH 8.5, 38°C
Proteus mirabilis
0.68
-
D-lactate
pH 8.5, 38°C
Proteus mirabilis
0.92
-
L-mannonate
pH 8.5, 38°C
Proteus mirabilis
0.98
-
D-gluconate
pH 8.5, 38°C
Proteus mirabilis
1.27
-
D-xylonate
pH 8.5, 38°C
Proteus mirabilis
1.46
-
L-arabinuronate
pH 8.5, 38°C
Proteus mirabilis
1.68
-
D-glucarate
pH 8.5, 38°C
Proteus mirabilis
2.03
-
L-arabinoic acid
pH 8.5, 38°C
Proteus mirabilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Proteus mirabilis
-
-
-
Reaction
Reaction
Commentary
Organism
a (2R)-hydroxy-carboxylate + acceptor = a 2-oxo-carboxylate + reduced acceptor
aldonates and aldarates with an (R)-configured alpha-carbon atom are converted to the corresponding 2-oxocarboxylates
Proteus mirabilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-phospho-D-gluconate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
6-phospho-D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
6-phospho-D-gluconate + oxidized benzyl viologen
-
654958
Proteus mirabilis
6-phospho-D-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
6-phospho-D-gluconate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
6-phospho-D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-galactonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-lyxo-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-galactonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-lyxo-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-galactonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-lyxo-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-glucarate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
2-oxo-D-glucarate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-glucarate + oxidized benzyl viologen
-
654958
Proteus mirabilis
2-oxo-D-glucarate + reduced benzyl viologen
-
-
-
r
D-glucarate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
2-oxo-D-glucarate + reduced carboxamido-methyl viologen
-
-
-
r
D-gluconate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-gluconate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-gluconate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-gulonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-gulonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-gulonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-lactate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
pyruvate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-lactate + oxidized benzyl viologen
-
654958
Proteus mirabilis
pyruvate + reduced benzyl viologen
-
-
-
r
D-lactate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
pyruvate + reduced carboxamido-methyl viologen
-
-
-
r
D-ribonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-erythro-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-ribonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-erythro-pent-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-ribonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-erythro-pent-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-xylonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-threo-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-xylonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-threo-pent-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-xylonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-threo-pent-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
galactarate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
2-oxogalactarate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
galactarate + oxidized benzyl viologen
-
654958
Proteus mirabilis
2-oxogalactarate + reduced benzyl viologen
-
-
-
r
galactarate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
2-oxogalactarate + reduced carboxamido-methyl viologen
-
-
-
r
L-arabinonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
L-erythro-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
L-arabinonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
L-erythro-pent-2-ulosonate + reduced benzyl viologen
-
-
-
r
L-arabinonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
L-erythro-pent-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
L-arabinuronate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
? + reduced anthraquinone-2,6-disulfonate
-
-
-
r
L-arabinuronate + oxidized benzyl viologen
-
654958
Proteus mirabilis
? + reduced benzyl viologen
-
-
-
r
L-arabinuronate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
? + reduced carboxamido-methyl viologen
-
-
-
r
L-mannonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
L-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
L-mannonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
L-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
L-mannonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
L-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
lactobionate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
4-O-(beta-D-galactopyranosido)-D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
lactobionate + oxidized benzyl viologen
-
654958
Proteus mirabilis
4-O-(beta-D-galactopyranosido)-D-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
lactobionate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
4-O-(beta-D-galactopyranosido)-D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
additional information
product determination, stereochemistry and molecule conformations, products appear in pyranose, furanose or lactone form, overview
654958
Proteus mirabilis
?
-
-
-
-
phenylpyruvate + reduced carbamoylmethyl viologen
-
654958
Proteus mirabilis
(2R)-phenyllactate + oxidized carbamoylmethyl viologen
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Proteus mirabilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Proteus mirabilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
carboxamido-methyl viologen
pH 8.5, 38°C
Proteus mirabilis
0.042
-
benzyl viologen
pH 8.5, 38°C
Proteus mirabilis
0.19
-
oxidized antraquinone-2,6-disulfonate
pH 8.5, 38°C
Proteus mirabilis
0.3
-
D-ribonate
pH 8.5, 38°C
Proteus mirabilis
0.42
-
galactarate
pH 8.5, 38°C
Proteus mirabilis
0.48
-
D-galactonate
pH 8.5, 38°C
Proteus mirabilis
0.64
-
6-phospho-D-gluconate
pH 8.5, 38°C
Proteus mirabilis
0.64
-
D-gulonate
pH 8.5, 38°C
Proteus mirabilis
0.66
-
Lactobionate
pH 8.5, 38°C
Proteus mirabilis
0.68
-
D-lactate
pH 8.5, 38°C
Proteus mirabilis
0.92
-
L-mannonate
pH 8.5, 38°C
Proteus mirabilis
0.98
-
D-gluconate
pH 8.5, 38°C
Proteus mirabilis
1.27
-
D-xylonate
pH 8.5, 38°C
Proteus mirabilis
1.46
-
L-arabinuronate
pH 8.5, 38°C
Proteus mirabilis
1.68
-
D-glucarate
pH 8.5, 38°C
Proteus mirabilis
2.03
-
L-arabinoic acid
pH 8.5, 38°C
Proteus mirabilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-phospho-D-gluconate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
6-phospho-D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
6-phospho-D-gluconate + oxidized benzyl viologen
-
654958
Proteus mirabilis
6-phospho-D-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
6-phospho-D-gluconate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
6-phospho-D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-galactonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-lyxo-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-galactonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-lyxo-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-galactonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-lyxo-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-glucarate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
2-oxo-D-glucarate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-glucarate + oxidized benzyl viologen
-
654958
Proteus mirabilis
2-oxo-D-glucarate + reduced benzyl viologen
-
-
-
r
D-glucarate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
2-oxo-D-glucarate + reduced carboxamido-methyl viologen
-
-
-
r
D-gluconate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-gluconate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-gluconate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-gulonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-gulonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-gulonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-lactate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
pyruvate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-lactate + oxidized benzyl viologen
-
654958
Proteus mirabilis
pyruvate + reduced benzyl viologen
-
-
-
r
D-lactate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
pyruvate + reduced carboxamido-methyl viologen
-
-
-
r
D-ribonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-erythro-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-ribonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-erythro-pent-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-ribonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-erythro-pent-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
D-xylonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
D-threo-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
D-xylonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
D-threo-pent-2-ulosonate + reduced benzyl viologen
-
-
-
r
D-xylonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
D-threo-pent-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
galactarate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
2-oxogalactarate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
galactarate + oxidized benzyl viologen
-
654958
Proteus mirabilis
2-oxogalactarate + reduced benzyl viologen
-
-
-
r
galactarate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
2-oxogalactarate + reduced carboxamido-methyl viologen
-
-
-
r
L-arabinonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
L-erythro-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
L-arabinonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
L-erythro-pent-2-ulosonate + reduced benzyl viologen
-
-
-
r
L-arabinonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
L-erythro-pent-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
L-arabinuronate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
? + reduced anthraquinone-2,6-disulfonate
-
-
-
r
L-arabinuronate + oxidized benzyl viologen
-
654958
Proteus mirabilis
? + reduced benzyl viologen
-
-
-
r
L-arabinuronate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
? + reduced carboxamido-methyl viologen
-
-
-
r
L-mannonate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
L-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
L-mannonate + oxidized benzyl viologen
-
654958
Proteus mirabilis
L-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
L-mannonate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
L-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
lactobionate + oxidized anthraquinone-2,6-disulfonate
-
654958
Proteus mirabilis
4-O-(beta-D-galactopyranosido)-D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
-
-
-
r
lactobionate + oxidized benzyl viologen
-
654958
Proteus mirabilis
4-O-(beta-D-galactopyranosido)-D-arabino-hex-2-ulosonate + reduced benzyl viologen
-
-
-
r
lactobionate + oxidized carboxamido-methyl viologen
-
654958
Proteus mirabilis
4-O-(beta-D-galactopyranosido)-D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
-
-
-
r
additional information
product determination, stereochemistry and molecule conformations, products appear in pyranose, furanose or lactone form, overview
654958
Proteus mirabilis
?
-
-
-
-
phenylpyruvate + reduced carbamoylmethyl viologen
-
654958
Proteus mirabilis
(2R)-phenyllactate + oxidized carbamoylmethyl viologen
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Proteus mirabilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Proteus mirabilis
Other publictions for EC 1.1.99.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287842
Hekmat
-
Production of pyruvate from (R ...
Proteus vulgaris
Enzyme Microb. Technol.
24
471-479
1999
-
2
-
-
-
-
-
-
-
-
-
-
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1
-
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-
-
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1
-
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-
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-
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2
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-
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-
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-
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
655353
Andersson
-
Microbial production of D-(S)- ...
Proteus vulgaris
Enzyme Microb. Technol.
22
170-178
1998
1
1
-
-
-
-
-
-
-
-
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1
-
-
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1
1
1
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1
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1
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1
1
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1
1
1
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1
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1
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1
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1
1
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7
2
2
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1
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2
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1
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2
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2
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1
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2
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1
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1
1
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7
2
2
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16
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1
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41
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1
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1
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16
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41
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1
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1
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655713
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3
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1
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1
2
5
1
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1
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2
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1
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2
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1
1
3
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1
2
5
1
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1
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655314
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1
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3
1
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1
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1
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1
2
18
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1
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1
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1
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3
1
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1
2
18
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1
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1
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644550
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2
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4
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2
1
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2
1
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47
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1
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2
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2
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2
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4
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1
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1
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47
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