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Literature summary for 1.1.99.30 extracted from
- The (2R)-hydroxycarboxylate-viologen-oxidoreductase from Proteus vulgaris is a molybdenum-containing iron-sulphur protein (1994), Eur. J. Biochem., 222, 1025-1032.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CN- | - |
Proteus vulgaris |  |
| O2 | oxygen-sensitive | Proteus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Proteus vulgaris | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
8 * 80000, SDS-PAGE | Proteus vulgaris |
| 600000 | - |
gel filtration | Proteus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Proteus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anaerobic conditions | Proteus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | anaerobic growth conditions | Proteus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1800 | - |
purified holoenzyme | Proteus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a (2R)-hydroxy-carboxylate + acceptor | - |
Proteus vulgaris | a 2-oxo-carboxylate + reduced acceptor | - |
? | |
| D-gluconate + oxidized 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | ? + reduced 1,1'-carbamoyl methyl viologen | - |
r | |
| D-glucose 6-phosphate + oxidized 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | ? + reduced 1,1'-carbamoyl methyl viologen | - |
r | |
| D-lactate + oxidized 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | pyruvate + reduced 1,1'-carbamoyl methyl viologen | - |
r | |
| N-acetylneuraminate + oxidized 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | ? + reduced 1,1'-carbamoyl methyl viologen | - |
r | |
| phenylpyruvate + reduced 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | phenyllactate + oxidized 1,1'-carbamoyl methyl viologen | - |
r | |
| phenylpyruvate + reduced benzyl viologen | anaerobic conditions | Proteus vulgaris | phenyllactate + oxidized benzyl viologen | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | subunit structure | Proteus vulgaris |
| octamer | 8 * 80000, SDS-PAGE | Proteus vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HVOR | - |
Proteus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
dehydrogenase assay at | Proteus vulgaris |
| 37 | - |
reductase assay at | Proteus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Proteus vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| molybdenum cofactor | mononucleotide, 1 enzyme molecule contains 1 molybdenum atom | Proteus vulgaris | |
| additional information | enzyme contains iron sulfur clusters, with four iron and 4 sulfur atoms per enzyme molecule, enzyme belongs to the group of molybdoenzymes which possess no further prosthetic group than the iron sulfur clusters | Proteus vulgaris |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Proteus vulgaris | isoelectric focusing | - |
4.9 |
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Release 2023.1 (January 2023)
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