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Literature summary for 1.1.99.3 extracted from
- A temperature-regulated Campylobacter jejuni gluconate dehydrogenase is involved in respiration-dependent energy conservation and chicken colonization (2008), Mol. Microbiol., 68, 474-491.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the cj0414-cj0415 PCR product is subcloned into pCRII-TOPO, cj0414 and cj0415 are cloned into the vector pBluescript II | Campylobacter jejuni |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| 81-176cj0414 | mutant, lacking the expression of the Cj0414 subunit | Campylobacter jejuni |
| 81-176cj0415 | mutant, lacking the expression of the Cj0415 subunit | Campylobacter jejuni |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Campylobacter jejuni | 5737 | - |
| membrane | - |
Campylobacter jejuni | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26900 | - |
predicted, subunit Cj0414 | Campylobacter jejuni |
| 63700 | - |
predicted, subunit Cj0415 | Campylobacter jejuni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate + acceptor | Campylobacter jejuni | - |
2-dehydro-D-gluconate + reduced acceptor | - |
? |  |
| D-gluconate + acceptor | Campylobacter jejuni 81-176 | - |
2-dehydro-D-gluconate + reduced acceptor | - |
? | |
| D-gluconate + oxidized 2,6-dichlorophenolindophenol | Campylobacter jejuni | activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS | 2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-gluconate + oxidized 2,6-dichlorophenolindophenol | Campylobacter jejuni 81-176 | activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS | 2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylobacter jejuni | Q0PB96 and Q0PB95 | subunit Cj0414 and subunit Cj0415 | - |
| Campylobacter jejuni 81-176 | Q0PB96 and Q0PB95 | subunit Cj0414 and subunit Cj0415 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0013 | - |
cells grown at 37°C, Cj0415 mutant | Campylobacter jejuni |
| 0.0019 | - |
cells grown at 42°C, Cj0414 mutant | Campylobacter jejuni |
| 0.0026 | - |
cells grown at 37°C, Cj0414 mutant | Campylobacter jejuni |
| 0.0045 | - |
cells grown at 42°C, Cj0415 mutant | Campylobacter jejuni |
| 0.0141 | - |
cells grown at 37°C, wild type | Campylobacter jejuni |
| 0.0333 | - |
cells grown at 42°C, wild type | Campylobacter jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate + acceptor | - |
Campylobacter jejuni | 2-dehydro-D-gluconate + reduced acceptor | - |
? | |
| D-gluconate + acceptor | - |
Campylobacter jejuni 81-176 | 2-dehydro-D-gluconate + reduced acceptor | - |
? | |
| D-gluconate + oxidized 2,6-dichlorophenolindophenol | activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS | Campylobacter jejuni | 2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-gluconate + oxidized 2,6-dichlorophenolindophenol | activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS | Campylobacter jejuni 81-176 | 2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | Cj0414 and Cj0415 subunit | Campylobacter jejuni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cj0414/Cj0415 | subunit Cj0414 and subunit Cj0415 | Campylobacter jejuni |
| GADH | - |
Campylobacter jejuni |
| gluconate dehydrogenase | - |
Campylobacter jejuni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Campylobacter jejuni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
activity assay | Campylobacter jejuni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | a putative NAD, FAD binding site is located within the first 48 amino acids of Cj0415 | Campylobacter jejuni |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Campylobacter jejuni | predicted, subunit Cj0414 | - |
5.5 |
| Campylobacter jejuni | predicted, subunit Cj0415 | - |
8.8 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Campylobacter jejuni | Cj0414 and Cj0415 are 3.4 and 2.9fold more highly expressed at 42°C compared to 37°C, respectively | up |
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Release 2023.1 (January 2023)
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