Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Agaricus bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus | |
0.079 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
0.17 | - |
ferrocenium ion | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus | |
0.566 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus | |
1.33 | - |
L-arabinose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
1.54 | - |
D-glucose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
2.16 | - |
maltose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
2.45 | - |
cellobiose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
4.19 | - |
D-galactose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
4.75 | - |
D-xylose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
10.79 | - |
raffinose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
40.51 | - |
lactose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
109.1 | - |
D-mannose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | Q3L1D1 | - |
- |
Agaricus bisporus DSM 3056 | Q3L1D1 | - |
- |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose column chromatography and DEAE-Sepharose column chromatography | Agaricus bisporus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Agaricus bisporus | - |
Storage Stability | Organism |
---|---|
25°C, 100 mM Britton Robinson buffer from pH 4.0-8.0, 1 week, no loss of activity | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + ferrocenium ion | - |
Agaricus bisporus | 2-dehydro-cellobiose + ferrocene | - |
? | |
cellobiose + ferrocenium ion | - |
Agaricus bisporus DSM 3056 | 2-dehydro-cellobiose + ferrocene | - |
? | |
D-galactose + ferrocenium ion | - |
Agaricus bisporus | 2-dehydro-D-galactose + ferrocene | - |
? | |
D-galactose + ferrocenium ion | - |
Agaricus bisporus DSM 3056 | 2-dehydro-D-galactose + ferrocene | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Agaricus bisporus | 2-dehydro-D-glucose + hydroquinone | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Agaricus bisporus DSM 3056 | 2-dehydro-D-glucose + hydroquinone | - |
? | |
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
Agaricus bisporus | 2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
? | |
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
Agaricus bisporus DSM 3056 | 2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Agaricus bisporus | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Agaricus bisporus DSM 3056 | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + ferrocenium ion | ferrocenium ion is the preferred electron acceptor | Agaricus bisporus | 2-dehydro-D-glucose + ferrocene | - |
? | |
D-mannose + ferrocenium ion | - |
Agaricus bisporus | 2-dehydro-D-mannose + ferrocene | - |
? | |
D-xylose + ferrocenium ion | - |
Agaricus bisporus | 2-dehydro-D-xylose + ferrocene | - |
? | |
L-arabinose + ferrocenium ion | - |
Agaricus bisporus | 2-dehydro-L-arabinose + ferrocene | - |
? | |
lactose + ferrocenium ion | - |
Agaricus bisporus | 2-dehydro-lactose + ferrocene | - |
? | |
maltose + ferrocenium ion | - |
Agaricus bisporus | 2-dehydro-maltose + ferrocene | - |
? | |
raffinose + ferrocenium ion | - |
Agaricus bisporus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000-100000, recombinant enzyme, SDS-PAGE | Agaricus bisporus |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Agaricus bisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
49 | - |
- |
Agaricus bisporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.31 | - |
D-mannose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
15.95 | - |
lactose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
19.61 | - |
raffinose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
25.63 | - |
cellobiose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
27.26 | - |
maltose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
28.7 | - |
D-galactose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
29.6 | - |
L-arabinose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
33.7 | - |
D-glucose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
36.9 | - |
D-xylose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
44.2 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus | |
57.6 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus | |
101.2 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
111.1 | - |
ferrocenium ion | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Agaricus bisporus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | more than 70% activity between pH 4.0 and 8.0 | Agaricus bisporus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | after 30 min at 50°C and standard assay pH (7.5) about 10% of initial recombinant enzyme activity is left while at pH 6.0 more than 90% remains active | Agaricus bisporus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
D-mannose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
0.4 | - |
lactose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
1.8 | - |
raffinose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
6.9 | - |
D-galactose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
7.8 | - |
D-xylose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
10 | - |
cellobiose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
13 | - |
maltose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
22 | - |
D-glucose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
22 | - |
L-arabinose | with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
78 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus | |
326 | - |
ferrocenium ion | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus | |
768 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C | Agaricus bisporus | |
958 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C | Agaricus bisporus |