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Literature summary for 1.1.99.29 extracted from

  • Gonaus, C.; Kittl, R.; Sygmund, C.; Haltrich, D.; Peterbauer, C.
    Transcription analysis of pyranose dehydrogenase from the basidiomycete Agaricus bisporus and characterization of the recombinantly expressed enzyme (2016), Protein Expr. Purif., 119, 36-44.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Agaricus bisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
0.079
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
0.17
-
ferrocenium ion with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
0.566
-
1,4-benzoquinone with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
1.33
-
L-arabinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.54
-
D-glucose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
2.16
-
maltose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
2.45
-
cellobiose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
4.19
-
D-galactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
4.75
-
D-xylose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
10.79
-
raffinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
40.51
-
lactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
109.1
-
D-mannose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus

Organism

Organism UniProt Comment Textmining
Agaricus bisporus Q3L1D1
-
-
Agaricus bisporus DSM 3056 Q3L1D1
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography and DEAE-Sepharose column chromatography Agaricus bisporus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Agaricus bisporus
-

Storage Stability

Storage Stability Organism
25°C, 100 mM Britton Robinson buffer from pH 4.0-8.0, 1 week, no loss of activity Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + ferrocenium ion
-
Agaricus bisporus 2-dehydro-cellobiose + ferrocene
-
?
cellobiose + ferrocenium ion
-
Agaricus bisporus DSM 3056 2-dehydro-cellobiose + ferrocene
-
?
D-galactose + ferrocenium ion
-
Agaricus bisporus 2-dehydro-D-galactose + ferrocene
-
?
D-galactose + ferrocenium ion
-
Agaricus bisporus DSM 3056 2-dehydro-D-galactose + ferrocene
-
?
D-glucose + 1,4-benzoquinone
-
Agaricus bisporus 2-dehydro-D-glucose + hydroquinone
-
?
D-glucose + 1,4-benzoquinone
-
Agaricus bisporus DSM 3056 2-dehydro-D-glucose + hydroquinone
-
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
Agaricus bisporus 2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
Agaricus bisporus DSM 3056 2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Agaricus bisporus 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Agaricus bisporus DSM 3056 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + ferrocenium ion ferrocenium ion is the preferred electron acceptor Agaricus bisporus 2-dehydro-D-glucose + ferrocene
-
?
D-mannose + ferrocenium ion
-
Agaricus bisporus 2-dehydro-D-mannose + ferrocene
-
?
D-xylose + ferrocenium ion
-
Agaricus bisporus 2-dehydro-D-xylose + ferrocene
-
?
L-arabinose + ferrocenium ion
-
Agaricus bisporus 2-dehydro-L-arabinose + ferrocene
-
?
lactose + ferrocenium ion
-
Agaricus bisporus 2-dehydro-lactose + ferrocene
-
?
maltose + ferrocenium ion
-
Agaricus bisporus 2-dehydro-maltose + ferrocene
-
?
raffinose + ferrocenium ion
-
Agaricus bisporus ?
-
?

Subunits

Subunits Comment Organism
? x * 75000-100000, recombinant enzyme, SDS-PAGE Agaricus bisporus

Synonyms

Synonyms Comment Organism
PDH
-
Agaricus bisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
49
-
-
Agaricus bisporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.31
-
D-mannose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
15.95
-
lactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
19.61
-
raffinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
25.63
-
cellobiose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
27.26
-
maltose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
28.7
-
D-galactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
29.6
-
L-arabinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
33.7
-
D-glucose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
36.9
-
D-xylose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
44.2
-
1,4-benzoquinone with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
57.6
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
101.2
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
111.1
-
ferrocenium ion with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Agaricus bisporus

pH Range

pH Minimum pH Maximum Comment Organism
4 8 more than 70% activity between pH 4.0 and 8.0 Agaricus bisporus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 after 30 min at 50°C and standard assay pH (7.5) about 10% of initial recombinant enzyme activity is left while at pH 6.0 more than 90% remains active Agaricus bisporus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
D-mannose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
0.4
-
lactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.8
-
raffinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
6.9
-
D-galactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
7.8
-
D-xylose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
10
-
cellobiose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
13
-
maltose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
22
-
D-glucose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
22
-
L-arabinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
78
-
1,4-benzoquinone with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
326
-
ferrocenium ion with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
768
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
958
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus