BRENDA - Enzyme Database
show all sequences of 1.1.99.29

Transcription analysis of pyranose dehydrogenase from the basidiomycete Agaricus bisporus and characterization of the recombinantly expressed enzyme

Gonaus, C.; Kittl, R.; Sygmund, C.; Haltrich, D.; Peterbauer, C.; Protein Expr. Purif. 119, 36-44 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Pichia pastoris
Agaricus bisporus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
0.079
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
0.17
-
ferrocenium ion
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
0.566
-
1,4-benzoquinone
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
1.33
-
L-arabinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
1.54
-
D-glucose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
2.16
-
maltose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
2.45
-
cellobiose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
4.19
-
D-galactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
4.75
-
D-xylose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
10.79
-
raffinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
40.51
-
lactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
109.1
-
D-mannose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
Organism
Organism
UniProt
Commentary
Textmining
Agaricus bisporus
Q3L1D1
-
-
Agaricus bisporus DSM 3056
Q3L1D1
-
-
Purification (Commentary)
Purification (Commentary)
Organism
phenyl Sepharose column chromatography and DEAE-Sepharose column chromatography
Agaricus bisporus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Agaricus bisporus
-
Storage Stability
Storage Stability
Organism
25C, 100 mM Britton Robinson buffer from pH 4.0-8.0, 1 week, no loss of activity
Agaricus bisporus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cellobiose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-cellobiose + ferrocene
-
-
-
?
cellobiose + ferrocenium ion
-
741362
Agaricus bisporus DSM 3056
2-dehydro-cellobiose + ferrocene
-
-
-
?
D-galactose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-galactose + ferrocenium ion
-
741362
Agaricus bisporus DSM 3056
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-glucose + 1,4-benzoquinone
-
741362
Agaricus bisporus
2-dehydro-D-glucose + hydroquinone
-
-
-
?
D-glucose + 1,4-benzoquinone
-
741362
Agaricus bisporus DSM 3056
2-dehydro-D-glucose + hydroquinone
-
-
-
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
741362
Agaricus bisporus
2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
-
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
741362
Agaricus bisporus DSM 3056
2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
741362
Agaricus bisporus
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
741362
Agaricus bisporus DSM 3056
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + ferrocenium ion
ferrocenium ion is the preferred electron acceptor
741362
Agaricus bisporus
2-dehydro-D-glucose + ferrocene
-
-
-
?
D-mannose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-D-mannose + ferrocene
-
-
-
?
D-xylose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-D-xylose + ferrocene
-
-
-
?
L-arabinose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-L-arabinose + ferrocene
-
-
-
?
lactose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-lactose + ferrocene
-
-
-
?
maltose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-maltose + ferrocene
-
-
-
?
raffinose + ferrocenium ion
-
741362
Agaricus bisporus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 75000-100000, recombinant enzyme, SDS-PAGE
Agaricus bisporus
Synonyms
Synonyms
Commentary
Organism
PDH
-
Agaricus bisporus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
49
-
-
Agaricus bisporus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13.31
-
D-mannose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
15.95
-
lactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
19.61
-
raffinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
25.63
-
cellobiose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
27.26
-
maltose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
28.7
-
D-galactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
29.6
-
L-arabinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
33.7
-
D-glucose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
36.9
-
D-xylose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
44.2
-
1,4-benzoquinone
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
57.6
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
101.2
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
111.1
-
ferrocenium ion
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Agaricus bisporus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
8
more than 70% activity between pH 4.0 and 8.0
Agaricus bisporus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.5
after 30 min at 50C and standard assay pH (7.5) about 10% of initial recombinant enzyme activity is left while at pH 6.0 more than 90% remains active
Agaricus bisporus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris
Agaricus bisporus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
0.079
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
0.17
-
ferrocenium ion
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
0.566
-
1,4-benzoquinone
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
1.33
-
L-arabinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
1.54
-
D-glucose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
2.16
-
maltose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
2.45
-
cellobiose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
4.19
-
D-galactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
4.75
-
D-xylose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
10.79
-
raffinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
40.51
-
lactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
109.1
-
D-mannose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
Purification (Commentary) (protein specific)
Commentary
Organism
phenyl Sepharose column chromatography and DEAE-Sepharose column chromatography
Agaricus bisporus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Agaricus bisporus
-
Storage Stability (protein specific)
Storage Stability
Organism
25C, 100 mM Britton Robinson buffer from pH 4.0-8.0, 1 week, no loss of activity
Agaricus bisporus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cellobiose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-cellobiose + ferrocene
-
-
-
?
cellobiose + ferrocenium ion
-
741362
Agaricus bisporus DSM 3056
2-dehydro-cellobiose + ferrocene
-
-
-
?
D-galactose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-galactose + ferrocenium ion
-
741362
Agaricus bisporus DSM 3056
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-glucose + 1,4-benzoquinone
-
741362
Agaricus bisporus
2-dehydro-D-glucose + hydroquinone
-
-
-
?
D-glucose + 1,4-benzoquinone
-
741362
Agaricus bisporus DSM 3056
2-dehydro-D-glucose + hydroquinone
-
-
-
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
741362
Agaricus bisporus
2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
-
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
741362
Agaricus bisporus DSM 3056
2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
741362
Agaricus bisporus
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
741362
Agaricus bisporus DSM 3056
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + ferrocenium ion
ferrocenium ion is the preferred electron acceptor
741362
Agaricus bisporus
2-dehydro-D-glucose + ferrocene
-
-
-
?
D-mannose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-D-mannose + ferrocene
-
-
-
?
D-xylose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-D-xylose + ferrocene
-
-
-
?
L-arabinose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-L-arabinose + ferrocene
-
-
-
?
lactose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-lactose + ferrocene
-
-
-
?
maltose + ferrocenium ion
-
741362
Agaricus bisporus
2-dehydro-maltose + ferrocene
-
-
-
?
raffinose + ferrocenium ion
-
741362
Agaricus bisporus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 75000-100000, recombinant enzyme, SDS-PAGE
Agaricus bisporus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
49
-
-
Agaricus bisporus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13.31
-
D-mannose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
15.95
-
lactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
19.61
-
raffinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
25.63
-
cellobiose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
27.26
-
maltose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
28.7
-
D-galactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
29.6
-
L-arabinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
33.7
-
D-glucose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
36.9
-
D-xylose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
44.2
-
1,4-benzoquinone
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
57.6
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
101.2
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
111.1
-
ferrocenium ion
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Agaricus bisporus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
8
more than 70% activity between pH 4.0 and 8.0
Agaricus bisporus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.5
after 30 min at 50C and standard assay pH (7.5) about 10% of initial recombinant enzyme activity is left while at pH 6.0 more than 90% remains active
Agaricus bisporus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.1
-
D-mannose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
0.4
-
lactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
1.8
-
raffinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
6.9
-
D-galactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
7.8
-
D-xylose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
10
-
cellobiose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
13
-
maltose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
22
-
D-glucose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
22
-
L-arabinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
78
-
1,4-benzoquinone
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
326
-
ferrocenium ion
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
768
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
958
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.1
-
D-mannose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
0.4
-
lactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
1.8
-
raffinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
6.9
-
D-galactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
7.8
-
D-xylose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
10
-
cellobiose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
13
-
maltose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
22
-
D-glucose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
22
-
L-arabinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
78
-
1,4-benzoquinone
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
326
-
ferrocenium ion
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
768
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
Agaricus bisporus
958
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30C
Agaricus bisporus
Other publictions for EC 1.1.99.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739926
Graf
Characterization of three pyra ...
Leucoagaricus meleagris
Appl. Microbiol. Biotechnol.
101
2879-2891
2017
-
-
1
-
-
-
-
36
-
-
1
-
-
8
-
1
1
-
-
-
-
-
36
1
3
2
-
1
36
1
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
37
-
-
3
-
-
-
3
3
-
-
-
-
36
3
3
-
3
36
3
-
-
-
-
-
-
-
36
36
740401
Gonaus
Analysis of Agaricus meleagris ...
Leucoagaricus meleagris
Enzyme Microb. Technol.
99
57-66
2017
-
-
1
-
6
-
-
40
-
-
1
-
-
2
-
1
1
-
-
-
-
-
4
1
2
-
-
-
40
-
-
-
1
-
-
-
-
-
1
1
-
6
-
-
-
-
40
-
-
1
-
-
-
1
1
-
-
-
-
4
1
-
-
-
40
-
-
-
-
-
-
-
-
-
-
741362
Gonaus
Transcription analysis of pyra ...
Agaricus bisporus, Agaricus bisporus DSM 3056
Protein Expr. Purif.
119
36-44
2016
-
-
1
-
-
-
-
13
-
-
-
-
-
6
-
-
1
-
-
1
-
1
17
1
1
1
-
-
13
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
1
-
1
-
1
17
1
1
-
-
13
1
1
1
-
-
-
-
-
13
13
740437
Graf
Reaction of pyranose dehydroge ...
Leucoagaricus meleagris
FEBS J.
282
4218-4241
2015
-
-
1
-
8
-
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741112
Yakovleva
Engineering of pyranose dehydr ...
Leucoagaricus meleagris
Phys. Chem. Chem. Phys.
17
9074-9081
2015
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
1
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739955
Krondorfer
Agaricus meleagris pyranose de ...
Leucoagaricus meleagris
Arch. Biochem. Biophys.
558
111-119
2014
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
740391
Cruys-Bagger
A pyranose dehydrogenase-based ...
Leucoagaricus meleagris
Enzyme Microb. Technol.
58-59
68-74
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741223
Graf
Pyranose dehydrogenase ligand ...
Leucoagaricus meleagris
PLoS Comput. Biol.
10
e1003995
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741303
Krondorfer
Engineering of pyranose dehydr ...
Leucoagaricus meleagris
PLoS ONE
9
e91145
2014
-
-
1
-
1
-
-
14
-
-
1
-
-
5
-
1
1
-
-
-
-
-
6
1
1
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
14
-
-
1
-
-
-
1
1
-
-
-
-
6
1
-
-
-
12
-
-
-
-
-
-
-
-
12
12
725611
Graf
Molecular dynamics simulations ...
Leucoagaricus meleagris
J. Comput. Aided Mol. Des.
27
295-304
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726317
Tan
The 1.6 A crystal structure of ...
Leucoagaricus meleagris
PLoS ONE
8
e53567
2013
-
-
1
1
-
-
-
-
-
-
-
4
-
5
-
-
1
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739847
Yakovleva
Further insights into the cata ...
Leucoagaricus meleagris
Anal. Chem.
85
9852-9858
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740138
Staudigl
Pyranose dehydrogenase from Ag ...
Agaricus campestris, Agaricus xanthodermus
Biomolecules
3
535-552
2013
-
-
2
-
-
-
-
16
-
-
4
-
-
7
-
2
2
-
-
-
-
-
14
2
2
-
-
-
16
4
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
16
-
-
4
-
-
-
2
2
-
-
-
-
14
2
-
-
-
16
4
-
-
-
-
-
-
-
16
16
724070
Sygmund
Simple and efficient expressio ...
Leucoagaricus meleagris
Appl. Microbiol. Biotechnol.
94
695-704
2012
-
-
1
-
-
-
-
16
-
-
3
7
-
6
-
1
1
-
-
-
2
-
7
1
3
-
-
-
16
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
16
-
-
3
7
-
-
1
1
-
-
2
-
7
1
-
-
-
16
-
-
-
-
-
-
-
-
16
16
724834
Yakovleva
-
Recombinant pyranose dehydroge ...
Leucoagaricus meleagris
Electrochem. Commun.
24
120-122
2012
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710969
Pisanelli
Heterologous expression of an ...
Leucoagaricus meleagris, Leucoagaricus meleagris CCBAS 907
Appl. Microbiol. Biotechnol.
86
599-606
2010
-
-
1
-
-
-
-
16
-
-
1
-
-
10
-
1
1
-
-
-
1
-
12
-
2
-
-
-
16
3
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
16
-
-
1
-
-
-
1
1
-
-
1
-
12
-
-
-
-
16
3
-
-
1
-
-
-
-
16
16
711340
Zafar
Wiring of pyranose dehydrogena ...
Leucoagaricus meleagris
Bioelectrochemistry
80
38-42
2010
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724043
Peterbauer
Pyranose dehydrogenases: Bioch ...
Agaricus xanthodermus, Chlorophyllum rhacodes, Leucoagaricus meleagris
Appl. Microbiol. Biotechnol.
85
837-848
2010
-
-
-
-
-
-
-
14
3
-
3
44
-
5
-
3
-
-
-
-
-
-
44
3
6
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
3
-
3
44
-
-
3
-
-
-
-
-
44
3
-
-
-
14
-
-
-
-
-
-
-
-
14
14
695788
Peterbauer
-
Pyranose dehydrogenases: bioch ...
Agaricus bisporus, Agaricus campestris, Agaricus xanthodermus, Chlorophyllum rhacodes, Leucoagaricus meleagris
Appl. Microbiol. Biotechnol.
146
491-499
2009
-
-
-
-
-
-
-
14
-
-
-
-
-
7
-
5
-
-
-
-
14
-
155
5
14
-
-
-
14
-
-
-
5
-
-
-
-
-
-
7
-
-
-
-
-
-
42
-
-
-
-
-
-
7
-
-
-
42
-
155
7
-
-
-
42
-
-
-
-
-
-
-
-
-
-
686261
Kittl
Molecular cloning of three pyr ...
Leucoagaricus meleagris
Curr. Genet.
53
117-127
2008
-
1
1
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687883
Sygmund
Characterization of pyranose d ...
Leucoagaricus meleagris
J. Biotechnol.
133
334-342
2008
1
1
-
-
-
-
9
20
1
11
2
-
-
3
-
1
1
-
-
1
-
1
21
1
3
1
-
1
-
2
1
1
1
-
4
-
1
1
-
1
-
-
-
-
9
-
20
1
11
2
-
-
-
1
1
-
1
-
1
21
1
1
-
1
-
2
1
1
4
-
-
-
-
-
-
686372
Tasca
-
Amperometric biosensors for de ...
Agaricus xanthodermus, Leucoagaricus meleagris
Electroanalysis
19
294-302
2007
-
2
-
-
-
-
-
18
2
-
-
-
-
2
-
-
2
-
-
-
-
-
18
-
6
-
-
-
-
2
4
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
18
2
-
-
-
-
-
-
2
-
-
-
-
18
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
686685
Kujawa
Properties of pyranose dehydro ...
Agaricus xanthodermus
FEBS J.
274
879-894
2007
-
1
-
-
-
-
-
21
1
-
1
-
-
4
-
1
1
-
-
-
-
1
22
1
3
1
-
-
-
3
1
1
1
-
1
-
-
1
-
1
-
-
-
-
-
-
21
1
-
1
-
-
-
1
1
-
-
-
1
22
1
1
-
-
-
3
1
1
1
-
-
-
-
-
-
675469
Sedmera
-
New biotransformations of some ...
Leucoagaricus meleagris
J. Mol. Catal. B
41
32-42
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644548
Volc
-
C-3 oxidation of non-reducing ...
Leucoagaricus meleagris, Leucoagaricus meleagris CCBAS 907
J. Mol. Catal. B
17
91-100
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
1
1
14
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
1
14
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644547
Volc
Screening of basidiomycete fun ...
Chlorophyllum rhacodes
Arch. Microbiol.
176
178-186
2001
-
-
-
-
-
1
-
4
1
-
2
-
-
7
-
1
1
-
-
3
1
1
37
1
-
2
-
-
-
2
-
-
2
-
1
-
-
-
-
2
-
-
1
-
-
-
4
1
-
2
-
-
-
1
1
-
3
1
1
37
1
2
-
-
-
2
-
-
1
-
-
-
-
-
-
644546
Volc
Double oxidation of D-xylose t ...
Agaricus bisporus, Agaricus bisporus 306
Carbohydr. Res.
329
219-225
2000
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
-
3
-
-
10
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644545
Volc
-
C-2 and C-3 oxidation of D-Glc ...
Agaricus bisporus, Agaricus bisporus U3
Carbohydr. Res.
310
151-156
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
-
-
10
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644544
Volc
Pyranose 2-dehydrogenase, a no ...
Agaricus bisporus
Arch. Microbiol.
167
119-125
1997
1
-
-
-
-
1
5
-
1
-
2
1
-
3
-
1
1
1
-
2
1
1
30
1
5
1
-
-
-
2
1
-
1
-
1
-
1
-
-
1
-
-
1
-
5
-
-
1
-
2
1
-
-
1
1
-
2
1
1
30
1
1
-
-
-
2
1
-
1
-
-
-
-
-
-