Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae and Pichia pastoris strain X33 | Leucoagaricus meleagris |
Protein Variants | Comment | Organism |
---|---|---|
H103Y | the mutant reveals a 5fold increase of the oxygen reactivity compared to the wild type enzyme | Leucoagaricus meleagris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2,6-dichlorophenolindophenol | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
0.095 | - |
O2 | wild type enzyme, with D-glucose as cosubstrate, at pH 7.4 and 30°C | Leucoagaricus meleagris | |
0.1 | - |
ferrocenium ion | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.14 | - |
2,6-dichlorophenolindophenol | wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
0.16 | - |
ferrocenium ion | wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.5 | - |
O2 | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 7.4 and 30°C | Leucoagaricus meleagris | |
0.69 | - |
D-glucose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
1.07 | - |
D-galactose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
1.38 | - |
1,4-benzoquinone | wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
3.64 | - |
1,4-benzoquinone | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
3.85 | - |
D-glucose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
5.29 | - |
D-galactose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
128 | - |
lactose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
395.9 | - |
lactose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, SDS-PAGE | Leucoagaricus meleagris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucoagaricus meleagris | Q3L245 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Leucoagaricus meleagris |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose column chromatography, DEAE Sepharose column chromatography, and gel filtration | Leucoagaricus meleagris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + ferrocenium ion | - |
Leucoagaricus meleagris | 2-dehydro-D-galactose + ferrocene | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Leucoagaricus meleagris | 2-dehydro-D-glucose + ? | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Leucoagaricus meleagris | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + ferrocenium ion | - |
Leucoagaricus meleagris | 2-dehydro-D-glucose + ferrocene | - |
? | |
D-glucose + O2 | - |
Leucoagaricus meleagris | 2-dehydro-D-glucose + H2O2 | - |
? | |
lactose + ferrocenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Leucoagaricus meleagris |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Leucoagaricus meleagris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
2,6-dichlorophenolindophenol | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
13.6 | - |
1,4-benzoquinone | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
24.7 | - |
lactose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
26 | - |
ferrocenium ion | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
27.4 | - |
D-glucose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
28.9 | - |
D-galactose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
37.8 | - |
D-glucose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
40.7 | - |
2,6-dichlorophenolindophenol | wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
41 | - |
lactose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
47.3 | - |
D-galactose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
65.4 | - |
1,4-benzoquinone | wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
130 | - |
ferrocenium ion | wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Leucoagaricus meleagris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
lactose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.32 | - |
lactose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
4 | - |
1,4-benzoquinone | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
5.5 | - |
D-galactose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
7.1 | - |
D-glucose | mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
44.2 | - |
D-galactose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
47.4 | - |
1,4-benzoquinone | wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
54.8 | - |
D-glucose | wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
140 | - |
2,6-dichlorophenolindophenol | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
260 | - |
ferrocenium ion | mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
290.7 | - |
2,6-dichlorophenolindophenol | wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
812.5 | - |
ferrocenium ion | wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C | Leucoagaricus meleagris |