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Literature summary for 1.1.99.29 extracted from

  • Krondorfer, I.; Lipp, K.; Brugger, D.; Staudigl, P.; Sygmund, C.; Haltrich, D.; Peterbauer, C.K.
    Engineering of pyranose dehydrogenase for increased oxygen reactivity (2014), PLoS ONE, 9, e91145.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae and Pichia pastoris strain X33 Leucoagaricus meleagris

Protein Variants

Protein Variants Comment Organism
H103Y the mutant reveals a 5fold increase of the oxygen reactivity compared to the wild type enzyme Leucoagaricus meleagris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
2,6-dichlorophenolindophenol mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
0.095
-
O2 wild type enzyme, with D-glucose as cosubstrate, at pH 7.4 and 30°C Leucoagaricus meleagris
0.1
-
ferrocenium ion mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
0.14
-
2,6-dichlorophenolindophenol wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
0.16
-
ferrocenium ion wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
0.5
-
O2 mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 7.4 and 30°C Leucoagaricus meleagris
0.69
-
D-glucose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.07
-
D-galactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.38
-
1,4-benzoquinone wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
3.64
-
1,4-benzoquinone mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
3.85
-
D-glucose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
5.29
-
D-galactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
128
-
lactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
395.9
-
lactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
x * 90000, SDS-PAGE Leucoagaricus meleagris

Organism

Organism UniProt Comment Textmining
Leucoagaricus meleagris Q3L245
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Leucoagaricus meleagris

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography, DEAE Sepharose column chromatography, and gel filtration Leucoagaricus meleagris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + ferrocenium ion
-
Leucoagaricus meleagris 2-dehydro-D-galactose + ferrocene
-
?
D-glucose + 1,4-benzoquinone
-
Leucoagaricus meleagris 2-dehydro-D-glucose + ?
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Leucoagaricus meleagris 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + ferrocenium ion
-
Leucoagaricus meleagris 2-dehydro-D-glucose + ferrocene
-
?
D-glucose + O2
-
Leucoagaricus meleagris 2-dehydro-D-glucose + H2O2
-
?
lactose + ferrocenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Leucoagaricus meleagris

Synonyms

Synonyms Comment Organism
PDH
-
Leucoagaricus meleagris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
2,6-dichlorophenolindophenol mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
13.6
-
1,4-benzoquinone mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
24.7
-
lactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
26
-
ferrocenium ion mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
27.4
-
D-glucose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
28.9
-
D-galactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
37.8
-
D-glucose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
40.7
-
2,6-dichlorophenolindophenol wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
41
-
lactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
47.3
-
D-galactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
65.4
-
1,4-benzoquinone wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
130
-
ferrocenium ion wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris

Cofactor

Cofactor Comment Organism Structure
FAD
-
Leucoagaricus meleagris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
lactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
0.32
-
lactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
4
-
1,4-benzoquinone mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
5.5
-
D-galactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
7.1
-
D-glucose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
44.2
-
D-galactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
47.4
-
1,4-benzoquinone wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
54.8
-
D-glucose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
140
-
2,6-dichlorophenolindophenol mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
260
-
ferrocenium ion mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
290.7
-
2,6-dichlorophenolindophenol wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
812.5
-
ferrocenium ion wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris