Literature summary for 1.1.99.29 extracted from

Graf, M.M.; Sucharitakul, J.; Bren, U.; Chu, D.B.; Koellensperger, G.; Hann, S.; Furtmueller, P.G.; Obinger, C.; Peterbauer, C.K.; Oostenbrink, C.; Chaiyen, P.; Haltrich, D.
Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates (2015), FEBS J., 282, 4218-4241.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris cells	Leucoagaricus meleagris

Protein Variants

Protein Variants	Comment	Organism
H103A	the mutant shows a reduction in catalytic efficiency for glucose by a factor of 10 compared to the wild type enzyme	Leucoagaricus meleagris
H512A	the mutant shows a reduction in catalytic efficiency for glucose by a factor of 100000 compared to the wild type enzyme	Leucoagaricus meleagris
H556A	the mutant shows a reduction in catalytic efficiency for glucose by a factor of 100 compared to the wild type enzyme	Leucoagaricus meleagris
H556N	the mutant shows a reduction in catalytic efficiency for glucose by a factor of 1000 compared to the wild type enzyme	Leucoagaricus meleagris
Q392A	the mutant shows a reduction in catalytic efficiency for glucose by a factor of 10 compared to the wild type enzyme	Leucoagaricus meleagris
V511F	the mutant shows a reduction in catalytic efficiency for glucose by a factor of 100 compared to the wild type enzyme	Leucoagaricus meleagris
V511W	the mutant shows a reduction in catalytic efficiency for glucose by a factor of 10 compared to the wild type enzyme	Leucoagaricus meleagris
Y510A	the mutant shows a reduction in catalytic efficiency for glucose by a factor of 100 compared to the wild type enzyme	Leucoagaricus meleagris

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90000	-	x * 90000, SDS-PAGE	Leucoagaricus meleagris

Organism

Organism	UniProt	Comment	Textmining
Leucoagaricus meleagris	Q3L245	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	approximately 30% degree of glycosylation	Leucoagaricus meleagris

Purification (Commentary)

Purification (Comment)	Organism
cross-flow filtration, hydrophobic interaction and immobilized metal affinity chromatography	Leucoagaricus meleagris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose + ferrocenium ion	-	Leucoagaricus meleagris	2-dehydro-D-glucose + ferrocene	-	?

Subunits

Subunits	Comment	Organism
?	x * 90000, SDS-PAGE	Leucoagaricus meleagris

Synonyms

Synonyms	Comment	Organism
PDH	-	Leucoagaricus meleagris

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Leucoagaricus meleagris

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Release 2023.1 (January 2023)