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Literature summary for 1.1.99.29 extracted from

  • Gonaus, C.; Maresch, D.; Schropp, K.; O Conghaile, P.; Leech, D.; Gorton, L.; Peterbauer, C.K.
    Analysis of Agaricus meleagris pyranose dehydrogenase N-glycosylation sites and performance of partially non-glycosylated enzymes (2017), Enzyme Microb. Technol., 99, 57-66.
    View publication on PubMed
Search for more literature for 1.1.99.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X33 cells Leucoagaricus meleagris

Protein Variants

Protein Variants Comment Organism
N175Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
N175Q/N252Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
N252Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
N75G N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
N75G/N175Q/N252Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris
N75G/N252Q N-glycosylation site knock out mutant with reduced activity compares to the wild type enzyme Leucoagaricus meleagris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 ferrocenium ion mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.11
-
 ferrocenium ion mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.24
-
 ferrocenium ion mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
0.27
-
 ferrocenium ion wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
0.29
-
 ferrocenium ion mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.3
-
 ferrocenium ion mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.37
-
 ferrocenium ion mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.39
-
 ferrocenium ion mutant enzyme N75Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.58
-
 D-glucose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.72
-
 D-glucose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.8
-
 D-galactose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.86
-
 D-galactose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.87
-
 D-glucose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
0.88
-
 D-glucose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
0.97
-
 D-glucose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1
-
 D-galactose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1
-
 D-galactose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.14
-
 D-galactose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.5
-
 D-glucose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
1.85
-
 D-glucose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
1.94
-
 D-glucose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
2.19
-
 D-galactose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
3.09
-
 D-galactose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
3.55
-
 D-galactose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
4.74
-
 cellobiose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
5.52
-
 cellobiose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
5.7
-
 cellobiose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
5.72
-
 cellobiose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
6.38
-
 cellobiose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
6.69
-
 cellobiose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
7.1
-
 cellobiose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
8.13
-
 cellobiose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
74.2
-
 D-mannose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
83.7
-
 D-mannose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
87.9
-
 D-mannose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
116
-
 D-mannose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
142
-
 D-mannose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
227
-
 D-mannose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
355
-
 D-mannose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
380
-
 D-mannose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Leucoagaricus meleagris

Organism

Organism UniProt Comment Textmining
Leucoagaricus meleagris Q3L245
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Leucoagaricus meleagris

Purification (Commentary)

Purification (Comment) Organism
anion exchange column chromatography and Superdex 75 gel filtration Leucoagaricus meleagris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-cellobiose + ferrocene
-
 ?
D-galactose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-galactose + ferrocene
-
 ?
D-glucose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-glucose + ferrocene
-
 ?
D-mannose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-mannose + ferrocene
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Leucoagaricus meleagris

Synonyms

Synonyms Comment Organism
PDH1
-
 Leucoagaricus meleagris
pyranose dehydrogenase 1
-
 Leucoagaricus meleagris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 D-glucose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
22.1
-
 D-mannose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
25.7
-
 D-galactose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
27
-
 D-mannose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
27.4
-
 D-mannose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
29.1
-
 cellobiose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
30.9
-
 cellobiose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
31.3
-
 cellobiose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
31.5
-
 D-mannose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
31.8
-
 D-galactose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
33
-
 cellobiose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
33.5
-
 D-mannose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
34.2
-
 D-galactose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
34.7
-
 cellobiose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
35
-
 D-mannose mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
35.2
-
 cellobiose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
36.6
-
 D-glucose wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
37.9
-
 D-galactose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
38.9
-
 D-galactose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
39.3
-
 D-galactose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
40.3
-
 D-galactose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
40.8
-
 D-mannose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
40.8
-
 D-glucose mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
41.9
-
 D-mannose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
43.8
-
 cellobiose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
44.8
-
 ferrocenium ion mutant enzyme N75G/N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
45.3
-
 D-glucose mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
45.4
-
 D-glucose mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
45.9
-
 D-galactose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
48.1
-
 D-glucose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
49.7
-
 D-glucose mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
50.7
-
 ferrocenium ion mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
50.8
-
 D-glucose mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
54.2
-
 ferrocenium ion mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
54.6
-
 ferrocenium ion mutant enzyme N75G/N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
55.1
-
 cellobiose mutant enzyme N175Q, at pH 7.5 and 30°C Leucoagaricus meleagris
65.8
-
 ferrocenium ion mutant enzyme N75G, at pH 7.5 and 30°C Leucoagaricus meleagris
66.3
-
 ferrocenium ion mutant enzyme N252Q, at pH 7.5 and 30°C Leucoagaricus meleagris
87.4
-
 ferrocenium ion wild type enzyme, at pH 7.5 and 30°C Leucoagaricus meleagris
125
-
 ferrocenium ion mutant enzyme N75Q, at pH 7.5 and 30°C Leucoagaricus meleagris

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Leucoagaricus meleagris