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Literature summary for 1.1.99.29 extracted from

  • Graf, M.M.; Weber, S.; Kracher, D.; Kittl, R.; Sygmund, C.; Ludwig, R.; Peterbauer, C.; Haltrich, D.
    Characterization of three pyranose dehydrogenase isoforms from the litter-decomposing basidiomycete Leucoagaricus meleagris (syn. Agaricus meleagris) (2017), Appl. Microbiol. Biotechnol., 101, 2879-2891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X33 Leucoagaricus meleagris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0103
-
ferricenium ion isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
0.0132
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
0.0201
-
ferricenium ion isoform PDH2, at pH 8.5 and 30°C Leucoagaricus meleagris
0.0325
-
1,4-benzoquinone isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
0.0791
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
0.109
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
0.161
-
ferricenium ion isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
0.53
-
D-glucose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.64
-
D-galactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.66
-
methyl-alpha-D-glucopyranoside isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.82
-
D-glucose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.95
-
methyl-alpha-D-glucopyranoside isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.38
-
1,4-benzoquinone isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.88
-
1,4-benzoquinone isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
2
-
methyl-alpha-D-glucopyranoside isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
2.22
-
maltose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
2.39
-
D-xylose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
3.28
-
D-glucose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
9.34
-
D-xylose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
10.3
-
maltose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
11.6
-
maltose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
17
-
D-xylose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
18.3
-
D-allose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
29.7
-
D-galactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
34.9
-
D-mannose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
40.1
-
D-galactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
54.6
-
maltotriose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
62.5
-
maltotriose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
76.7
-
lactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
94
-
D-mannose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
113
-
D-allose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
154
-
maltotriose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
181
-
D-allose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
185
-
D-mannose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
235
-
lactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
2288
-
lactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Leucoagaricus meleagris

Organism

Organism UniProt Comment Textmining
Leucoagaricus meleagris Q0R4L2 synonym Leucoagaricus meleagris
-
Leucoagaricus meleagris Q3L243 synonym Leucoagaricus meleagris
-
Leucoagaricus meleagris Q3L245 synonym Leucoagaricus meleagris
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Leucoagaricus meleagris

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni2+-charged chelating Sepharose column chromatography, and DEAE Sepharose column chromatography Leucoagaricus meleagris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
D-galactose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
D-glucose + 1,4-benzoquinone
-
Leucoagaricus meleagris 2-dehydro-D-glucose + ?
-
?
D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
-
Leucoagaricus meleagris 2-dehydro-D-glucose + ?
-
?
D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
-
Leucoagaricus meleagris 3-dehydro-D-glucose + ?
-
?
D-glucose + ferricenium ion
-
Leucoagaricus meleagris 2-dehydro-D-glucose + ferrocene
-
?
D-mannose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
D-xylose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
lactose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
maltose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
maltotriose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
methyl-alpha-D-glucopyranoside + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Leucoagaricus meleagris

Synonyms

Synonyms Comment Organism
PDH1 isoform Leucoagaricus meleagris
PDH2 isoform Leucoagaricus meleagris
PDH3 isoform Leucoagaricus meleagris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Leucoagaricus meleagris
52
-
-
Leucoagaricus meleagris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
66 73 isoform PDH1 shows its highest thermal stability between pH values 5.0 and 6.0, with maximum melting temperature at 73.5°C and pH 5.0 Leucoagaricus meleagris
66 73 isoform PDH2 shows a significantly reduced thermostability over the whole pH range with a maximum melting temperature of 66.5°C at pH 6.0 Leucoagaricus meleagris
66 73 isoform PDH3 shows its highest thermal stability between pH values 5.0 and 6.0, with maximum melting temperature at 73°C and pH 6.0 Leucoagaricus meleagris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
maltose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1
-
methyl-alpha-D-glucopyranoside isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
3.57
-
1,4-benzoquinone isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
4.28
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
5.32
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
7.57
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
7.63
-
maltose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
8.16
-
D-glucose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
9.86
-
ferricenium ion isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
16.3
-
maltotriose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
18.4
-
D-allose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
19.3
-
D-galactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
21.2
-
maltotriose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
23.4
-
1,4-benzoquinone isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
26.5
-
D-mannose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
29.5
-
D-xylose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
33.7
-
lactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
34.3
-
D-galactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
34.7
-
D-mannose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
35.3
-
D-allose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
35.7
-
D-glucose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
35.9
-
maltotriose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
40.2
-
D-allose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
41.5
-
methyl-alpha-D-glucopyranoside isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
42.2
-
D-glucose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
44.7
-
maltose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
48.1
-
ferricenium ion isoform PDH2, at pH 8.5 and 30°C Leucoagaricus meleagris
51.3
-
D-xylose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
65.4
-
1,4-benzoquinone isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
75.3
-
lactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
85
-
methyl-alpha-D-glucopyranoside isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
109
-
D-mannose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
130
-
ferricenium ion isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
137
-
D-galactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
146
-
D-xylose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
209
-
lactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Leucoagaricus meleagris

Cofactor

Cofactor Comment Organism Structure
FAD
-
Leucoagaricus meleagris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0913
-
lactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.106
-
maltotriose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.188
-
D-mannose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.195
-
D-allose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.216
-
maltose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.282
-
D-mannose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.32
-
lactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.356
-
D-allose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.388
-
maltotriose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.439
-
lactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.574
-
maltotriose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.65
-
D-galactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
0.658
-
maltose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.01
-
D-allose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.05
-
methyl-alpha-D-glucopyranoside isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.89
-
1,4-benzoquinone isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
2.48
-
D-glucose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
3.12
-
D-mannose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
3.16
-
D-xylose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
3.41
-
D-galactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
4.34
-
maltose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
8.59
-
D-xylose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
21.5
-
D-xylose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
38.9
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
42.5
-
methyl-alpha-D-glucopyranoside isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
47.4
-
1,4-benzoquinone isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
51.5
-
D-glucose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
53.6
-
D-galactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
62.9
-
methyl-alpha-D-glucopyranoside isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
67.4
-
D-glucose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
94.6
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
532
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
780
-
1,4-benzoquinone isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
812
-
ferricenium ion isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
986
-
ferricenium ion isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
2400
-
ferricenium ion isoform PDH2, at pH 8.5 and 30°C Leucoagaricus meleagris