Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X33 | Leucoagaricus meleagris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
ferricenium ion | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
0.0132 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
0.0201 | - |
ferricenium ion | isoform PDH2, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.0325 | - |
1,4-benzoquinone | isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
0.0791 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
0.109 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
0.161 | - |
ferricenium ion | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
0.53 | - |
D-glucose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.64 | - |
D-galactose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.66 | - |
methyl-alpha-D-glucopyranoside | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.82 | - |
D-glucose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.95 | - |
methyl-alpha-D-glucopyranoside | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
1.38 | - |
1,4-benzoquinone | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
1.88 | - |
1,4-benzoquinone | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
2 | - |
methyl-alpha-D-glucopyranoside | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
2.22 | - |
maltose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
2.39 | - |
D-xylose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
3.28 | - |
D-glucose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
9.34 | - |
D-xylose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
10.3 | - |
maltose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
11.6 | - |
maltose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
17 | - |
D-xylose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
18.3 | - |
D-allose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
29.7 | - |
D-galactose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
34.9 | - |
D-mannose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
40.1 | - |
D-galactose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
54.6 | - |
maltotriose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
62.5 | - |
maltotriose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
76.7 | - |
lactose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
94 | - |
D-mannose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
113 | - |
D-allose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
154 | - |
maltotriose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
181 | - |
D-allose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
185 | - |
D-mannose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
235 | - |
lactose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
2288 | - |
lactose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Leucoagaricus meleagris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucoagaricus meleagris | Q0R4L2 | synonym Leucoagaricus meleagris | - |
Leucoagaricus meleagris | Q3L243 | synonym Leucoagaricus meleagris | - |
Leucoagaricus meleagris | Q3L245 | synonym Leucoagaricus meleagris | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Leucoagaricus meleagris |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni2+-charged chelating Sepharose column chromatography, and DEAE Sepharose column chromatography | Leucoagaricus meleagris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
D-galactose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Leucoagaricus meleagris | 2-dehydro-D-glucose + ? | - |
? | |
D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | - |
Leucoagaricus meleagris | 2-dehydro-D-glucose + ? | - |
? | |
D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | - |
Leucoagaricus meleagris | 3-dehydro-D-glucose + ? | - |
? | |
D-glucose + ferricenium ion | - |
Leucoagaricus meleagris | 2-dehydro-D-glucose + ferrocene | - |
? | |
D-mannose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
D-xylose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
lactose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
maltose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
maltotriose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
methyl-alpha-D-glucopyranoside + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Leucoagaricus meleagris |
Synonyms | Comment | Organism |
---|---|---|
PDH1 | isoform | Leucoagaricus meleagris |
PDH2 | isoform | Leucoagaricus meleagris |
PDH3 | isoform | Leucoagaricus meleagris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Leucoagaricus meleagris |
52 | - |
- |
Leucoagaricus meleagris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | 73 | isoform PDH1 shows its highest thermal stability between pH values 5.0 and 6.0, with maximum melting temperature at 73.5°C and pH 5.0 | Leucoagaricus meleagris |
66 | 73 | isoform PDH2 shows a significantly reduced thermostability over the whole pH range with a maximum melting temperature of 66.5°C at pH 6.0 | Leucoagaricus meleagris |
66 | 73 | isoform PDH3 shows its highest thermal stability between pH values 5.0 and 6.0, with maximum melting temperature at 73°C and pH 6.0 | Leucoagaricus meleagris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
maltose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
1 | - |
methyl-alpha-D-glucopyranoside | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
3.57 | - |
1,4-benzoquinone | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
4.28 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
5.32 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
7.57 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
7.63 | - |
maltose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
8.16 | - |
D-glucose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
9.86 | - |
ferricenium ion | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
16.3 | - |
maltotriose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
18.4 | - |
D-allose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
19.3 | - |
D-galactose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
21.2 | - |
maltotriose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
23.4 | - |
1,4-benzoquinone | isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
26.5 | - |
D-mannose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
29.5 | - |
D-xylose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
33.7 | - |
lactose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
34.3 | - |
D-galactose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
34.7 | - |
D-mannose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
35.3 | - |
D-allose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
35.7 | - |
D-glucose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
35.9 | - |
maltotriose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
40.2 | - |
D-allose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
41.5 | - |
methyl-alpha-D-glucopyranoside | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
42.2 | - |
D-glucose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
44.7 | - |
maltose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
48.1 | - |
ferricenium ion | isoform PDH2, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
51.3 | - |
D-xylose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
65.4 | - |
1,4-benzoquinone | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
75.3 | - |
lactose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
85 | - |
methyl-alpha-D-glucopyranoside | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
109 | - |
D-mannose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
130 | - |
ferricenium ion | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
137 | - |
D-galactose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
146 | - |
D-xylose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
209 | - |
lactose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Leucoagaricus meleagris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Leucoagaricus meleagris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0913 | - |
lactose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.106 | - |
maltotriose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.188 | - |
D-mannose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.195 | - |
D-allose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.216 | - |
maltose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.282 | - |
D-mannose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.32 | - |
lactose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.356 | - |
D-allose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.388 | - |
maltotriose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.439 | - |
lactose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.574 | - |
maltotriose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.65 | - |
D-galactose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
0.658 | - |
maltose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
1.01 | - |
D-allose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
1.05 | - |
methyl-alpha-D-glucopyranoside | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
1.89 | - |
1,4-benzoquinone | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
2.48 | - |
D-glucose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
3.12 | - |
D-mannose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
3.16 | - |
D-xylose | isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
3.41 | - |
D-galactose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
4.34 | - |
maltose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
8.59 | - |
D-xylose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
21.5 | - |
D-xylose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
38.9 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
42.5 | - |
methyl-alpha-D-glucopyranoside | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
47.4 | - |
1,4-benzoquinone | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
51.5 | - |
D-glucose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
53.6 | - |
D-galactose | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
62.9 | - |
methyl-alpha-D-glucopyranoside | isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
67.4 | - |
D-glucose | isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Leucoagaricus meleagris | |
94.6 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
532 | - |
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
780 | - |
1,4-benzoquinone | isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
812 | - |
ferricenium ion | isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
986 | - |
ferricenium ion | isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
2400 | - |
ferricenium ion | isoform PDH2, at pH 8.5 and 30°C | Leucoagaricus meleagris |