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Literature summary for 1.1.99.29 extracted from

  • Sygmund, C.; Gutmann, A.; Krondorfer, I.; Kujawa, M.; Glieder, A.; Pscheidt, B.; Haltrich, D.; Peterbauer, C.; Kittl, R.
    Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris (2012), Appl. Microbiol. Biotechnol., 94, 695-704.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain CBS 7435 Leucoagaricus meleagris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
ferrocenium ion native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
0.16
-
ferrocenium ion recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
0.49
-
L-arabinose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
0.54
-
L-arabinose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
0.55
-
2-chloro-1,4-benzoquinone native enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
0.62
-
2-chloro-1,4-benzoquinone recombinant enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
0.69
-
D-glucose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
0.82
-
D-glucose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.05
-
D-galactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.07
-
D-galactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.38
-
1,4-benzoquinone recombinant enzyme, at pH 4.0 and 30°C Leucoagaricus meleagris
1.82
-
1,4-benzoquinone native enzyme, at pH 3.0 and 30°C Leucoagaricus meleagris
1.92
-
D-xylose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.93
-
D-xylose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
128
-
lactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
134
-
lactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61967
-
x * 61967, calculated from amino acid sequence Leucoagaricus meleagris
64000
-
x * 64000, deglycosylated protein, SDS-PAGE Leucoagaricus meleagris
93000
-
x * 93000, recombinant enzyme, SDS-PAGE Leucoagaricus meleagris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + ferrocenium ion Leucoagaricus meleagris
-
2-dehydro-D-galactose + ferrocene
-
?
D-glucose + 1,4-benzoquinone Leucoagaricus meleagris
-
?
-
?
D-glucose + 2-chloro-1,4-benzoquinone Leucoagaricus meleagris
-
?
-
?
D-glucose + ferrocenium ion Leucoagaricus meleagris
-
3-dehydro-D-glucose + ferrocene
-
?
D-xylose + ferrocenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
L-arabinose + ferrocenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
lactose + ferrocenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?

Organism

Organism UniProt Comment Textmining
Leucoagaricus meleagris Q3L245
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the level of glycosylation of recombinant enzyme is approximately 30% Leucoagaricus meleagris

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose column chromatography and phenyl Sepharose column chromatography Leucoagaricus meleagris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.5
-
culture supernatant, at pH 8.5 and 30°C Leucoagaricus meleagris
21.9
-
after 1.9fold purification, at pH 8.5 and 30°C Leucoagaricus meleagris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + ferrocenium ion
-
Leucoagaricus meleagris 2-dehydro-D-galactose + ferrocene
-
?
D-glucose + 1,4-benzoquinone
-
Leucoagaricus meleagris ?
-
?
D-glucose + 2-chloro-1,4-benzoquinone
-
Leucoagaricus meleagris ?
-
?
D-glucose + ferrocenium ion
-
Leucoagaricus meleagris 3-dehydro-D-glucose + ferrocene
-
?
D-xylose + ferrocenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
L-arabinose + ferrocenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
lactose + ferrocenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?

Subunits

Subunits Comment Organism
? x * 61967, calculated from amino acid sequence Leucoagaricus meleagris
? x * 64000, deglycosylated protein, SDS-PAGE Leucoagaricus meleagris
? x * 93000, recombinant enzyme, SDS-PAGE Leucoagaricus meleagris

Synonyms

Synonyms Comment Organism
PDH1
-
Leucoagaricus meleagris
pyranose dehydrogenase
-
Leucoagaricus meleagris
pyranose/acceptor oxidoreductase
-
Leucoagaricus meleagris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.9
-
2-chloro-1,4-benzoquinone recombinant enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
15.1
-
2-chloro-1,4-benzoquinone native enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
34.7
-
L-arabinose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
37.2
-
L-arabinose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
37.8
-
D-glucose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
39.6
-
lactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
41
-
lactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
43.4
-
D-xylose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
45.9
-
D-glucose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
47.3
-
D-galactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
48.5
-
D-galactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
62.4
-
D-xylose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
65.4
-
1,4-benzoquinone recombinant enzyme, at pH 4.0 and 30°C Leucoagaricus meleagris
76
-
1,4-benzoquinone native enzyme, at pH 3.0 and 30°C Leucoagaricus meleagris
104
-
ferrocenium ion native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
130
-
ferrocenium ion recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris

Cofactor

Cofactor Comment Organism Structure
flavin
-
Leucoagaricus meleagris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.29
-
lactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
0.32
-
lactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
20.9
-
2-chloro-1,4-benzoquinone recombinant enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
22.9
-
D-xylose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
25.5
-
2-chloro-1,4-benzoquinone native enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
32.5
-
D-xylose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
44.2
-
D-galactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
46.2
-
D-galactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
54.4
-
1,4-benzoquinone recombinant enzyme, at pH 4.0 and 30°C Leucoagaricus meleagris
54.8
-
D-glucose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
57.5
-
1,4-benzoquinone native enzyme, at pH 3.0 and 30°C Leucoagaricus meleagris
57.5
-
D-glucose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
62.1
-
L-arabinose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
70.8
-
L-arabinose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
802
-
ferrocenium ion native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
812
-
ferrocenium ion recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris