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Literature summary for 1.1.99.29 extracted from

  • Pisanelli, I.; Kujawa, M.; Gschnitzer, D.; Spadiut, O.; Seiboth, B.; Peterbauer, C.
    Heterologous expression of an Agaricus meleagris pyranose dehydrogenase-encoding gene in Aspergillus spp. and characterization of the recombinant enzyme (2010), Appl. Microbiol. Biotechnol., 86, 599-606.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus nidulans and Aspergillus niger Leucoagaricus meleagris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
ferricenium ion native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 Leucoagaricus meleagris
0.22
-
ferricenium ion recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 Leucoagaricus meleagris
0.44
-
L-arabinose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
0.55
-
2-chloro-1,4-benzoquinone native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 Leucoagaricus meleagris
0.61
-
2-chloro-1,4-benzoquinone recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 Leucoagaricus meleagris
0.74
-
L-arabinose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
0.82
-
D-glucose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
0.86
-
D-glucose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
1.02
-
D-galactose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
1.03
-
D-xylose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
1.03
-
1,4-benzoquinone recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 Leucoagaricus meleagris
1.05
-
D-galactose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
1.82
-
1,4-benzoquinone native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 Leucoagaricus meleagris
1.93
-
D-xylose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
113.9
-
lactose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
134
-
lactose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
SDS-PAGE Leucoagaricus meleagris

Organism

Organism UniProt Comment Textmining
Leucoagaricus meleagris Q3L245
-
-
Leucoagaricus meleagris CCBAS 907 Q3L245
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant protein contains approximately 7% N-linked carbohydrate moiety Leucoagaricus meleagris

Purification (Commentary)

Purification (Comment) Organism
EMD DEAE 650 S column chromatography and Source 15 PHE column chromatography Leucoagaricus meleagris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the recombinant enzyme from Aspergillus nidulans crude extract shows a specific activity of 13.5 units/mg using D-glucose as substrate, at 30°C in phosphate buffer, pH 7.5. The recombinant 4.1fold purified enzyme from Aspergillus nidulans shows a specific activity of 55.9 units/mg using D-glucose as substrate, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
D-galactose + ferricenium ion
-
Leucoagaricus meleagris CCBAS 907 ? + ferrocene
-
?
D-glucose + 1,4-benzoquinone
-
Leucoagaricus meleagris ?
-
?
D-glucose + 1,4-benzoquinone
-
Leucoagaricus meleagris CCBAS 907 ?
-
?
D-glucose + 2-chloro-1,4-benzoquinone
-
Leucoagaricus meleagris ?
-
?
D-glucose + 2-chloro-1,4-benzoquinone
-
Leucoagaricus meleagris CCBAS 907 ?
-
?
D-glucose + ferricenium ion
-
Leucoagaricus meleagris 3-dehydro-D-glucose + ferrocene
-
?
D-glucose + ferricenium ion
-
Leucoagaricus meleagris CCBAS 907 3-dehydro-D-glucose + ferrocene
-
?
D-xylose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
D-xylose + ferricenium ion
-
Leucoagaricus meleagris CCBAS 907 ? + ferrocene
-
?
L-arabinose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
lactose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?

Synonyms

Synonyms Comment Organism
PDH
-
Leucoagaricus meleagris
pyranose:acceptor oxidoreductase
-
Leucoagaricus meleagris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.9
-
2-chloro-1,4-benzoquinone recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 Leucoagaricus meleagris
15.1
-
2-chloro-1,4-benzoquinone native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 Leucoagaricus meleagris
31.9
-
lactose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
37.2
-
L-arabinose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
39.6
-
lactose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
41.5
-
D-xylose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
43.4
-
D-xylose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
45.7
-
L-arabinose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
45.9
-
D-glucose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
46.6
-
D-galactose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
48.06
-
D-glucose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
48.5
-
D-galactose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
59
-
1,4-benzoquinone recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 Leucoagaricus meleagris
76
-
1,4-benzoquinone native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 Leucoagaricus meleagris
104
-
ferricenium ion native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 Leucoagaricus meleagris
203
-
ferricenium ion recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 Leucoagaricus meleagris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
using 1,4-benzoquinone as electron acceptor Leucoagaricus meleagris
5
-
using 2-chloro-1,4-benzoquinone as electron acceptor Leucoagaricus meleagris
8.5
-
using ferrocenium ion as electron acceptor Leucoagaricus meleagris

pI Value

Organism Comment pI Value Maximum pI Value
Leucoagaricus meleagris isoelectric focusing 4.7 4.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.28
-
lactose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
0.29
-
lactose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
20.9
-
2-chloro-1,4-benzoquinone recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 Leucoagaricus meleagris
22.9
-
D-xylose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
25.5
-
2-chloro-1,4-benzoquinone native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 Leucoagaricus meleagris
40.3
-
D-xylose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
45.7
-
D-galactose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
46.2
-
D-galactose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
55.9
-
D-glucose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
57.3
-
1,4-benzoquinone recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 Leucoagaricus meleagris
57.5
-
D-glucose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
57.5
-
1,4-benzoquinone native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 Leucoagaricus meleagris
61.8
-
L-arabinose recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
62.1
-
L-arabinose native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 Leucoagaricus meleagris
802
-
ferricenium ion native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 Leucoagaricus meleagris
923
-
ferricenium ion recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 Leucoagaricus meleagris