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Literature summary for 1.1.99.29 extracted from

  • Kujawa, M.; Volc, J.; Halada, P.; Sedmera, P.; Divne, C.; Sygmund, C.; Leitner, C.; Peterbauer, C.; Haltrich, D.
    Properties of pyranose dehydrogenase purified from the litter-degrading fungus Agaricus xanthoderma (2007), FEBS J., 274, 879-894.
    View publication on PubMed
Search for more literature for 1.1.99.29

Application

Application Comment Organism
degradation lignocellulose degradation Agaricus xanthodermus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 3-ethylbenzthiazolin-6-sulfonic acid cation radical at 30°C with 25 mM glucose as substrate Agaricus xanthodermus
0.02
-
 3,5-di-t-butyl-1,2-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
0.05
-
 2,6-dichloroindophenol at 30°C with 25 mM glucose as substrate Agaricus xanthodermus
0.12
-
 ferricenium hexafluorophosphate at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
0.12
-
 2-methylcyclohexa-2,5-diene-1,4-dione at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
0.18
-
 2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
0.28
-
 tetrachloro-1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
0.33
-
 ferricyanide at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
0.39
-
 tetrafluoro-1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
0.42
-
 dimethylaminomethyl-ferricenium at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
0.78
-
 D-glucose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
0.95
-
 CuSO4 at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.1
-
 1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
1.6
-
 2-chloro-1,4-benzoquinone at 30°C with 25 mM glucose as susbtrate Agaricus xanthodermus
11
-
 D-galactose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
12
-
 D-maltose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
17
-
 D-arabinose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
17
-
 gentiobiose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
25
-
 D-xylose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
100
-
 D-maltotriose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus
120
-
 beta-lactose at 30°C with 0.4 mM ferricenium as electron acceptor Agaricus xanthodermus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Agaricus xanthodermus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65400
-
 SDS-PAGE, gel filtration, MALDI MS Agaricus xanthodermus

Organism

Organism UniProt Comment Textmining
Agaricus xanthodermus Q3L1D2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content of 5% Agaricus xanthodermus

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation and ion-exchange and hydrophobic interaction chromatography Agaricus xanthodermus

Storage Stability

Storage Stability Organism
several month at 4°, pH 6.5 in potassium phosphate buffer Agaricus xanthodermus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone
-
 Agaricus xanthodermus 2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone
-
 ?
beta-lactose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
D-arabinose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
D-galactose + benzoquinone
-
 Agaricus xanthodermus 2,3-didehydro-D-galactose + hydroquinone two main reaction products ?
D-galactose + benzoquinone
-
 Agaricus xanthodermus 2-dehydro-D-galactose + hydroquinone two main reaction products ?
D-glucose + 2,6-dichloroindophenol
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + 2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + 2-chloro-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + 3,5-di-t-butyl-1,2-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + 3-ethylbenzthiazolin-6-sulfonic acid cation radical
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + benzoquinone 3-oxo-D-glucose, 1-alpha-3-dehydro-d-glucopyranose, 1-beta-3-dehydro-d-glucopyranose, 1-alpha-3-dehydro-d-glucopyranose hydrate, 1-beta-3-dehydro-d-glucopyranose hydrate, 2-dehydro-d-glucose, 1-alpha-2,3-bis-dehydro-d-glucopyranose bis-hydrate as minor reaction products Agaricus xanthodermus 2,3-didehydro-D-glucose + hydroquinone
-
 ?
D-glucose + CuSO4
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + dimethylaminomethyl-ferricenium
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + Fe3+
-
 Agaricus xanthodermus 3-dehydro-D-glucose + Fe2+ + H+
-
 ?
D-glucose + ferricyanide
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + methyl-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + tetrachloro-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
D-glucose + tetrafluoro-1,4-benzoquinone
-
 Agaricus xanthodermus ?
-
 ?
D-maltose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
D-maltotriose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
D-xylose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?
gentiobiose + ferricenium hexafluorophosphate
-
 Agaricus xanthodermus ?
-
 ?

Subunits

Subunits Comment Organism
monomer gel filtration Agaricus xanthodermus

Synonyms

Synonyms Comment Organism
PDH
-
 Agaricus xanthodermus
pyranose dehydrogenase
-
 Agaricus xanthodermus
pyranose:acceptor oxidoreductase
-
 Agaricus xanthodermus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Agaricus xanthodermus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
 for glucose and 1,4-benzoquinone as substrates Agaricus xanthodermus
8
-
 for glucose and 1,4-benzoquinone as substrates Agaricus xanthodermus
8.5
-
 for glucose and ferricenium hexafluorophosphate as substrates Agaricus xanthodermus

pH Range

pH Minimum pH Maximum Comment Organism
2 10 depending on the electron acceptor and with D-glucose as the electron donor, has 2 pH optima for some substrates Agaricus xanthodermus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.7 7.7 in potassium phosphate buffer, half life of 126 days at pH 7.7 Agaricus xanthodermus

Cofactor

Cofactor Comment Organism Structure
FAD covalent type of flavin-polypeptide linkage, no release by treatment with 5% trichloric acid and heating Agaricus xanthodermus

pI Value

Organism Comment pI Value Maximum pI Value
Agaricus xanthodermus isoelectric focusing, five isoforms
-
 4.2