Application | Comment | Organism |
---|---|---|
biotechnology | PDH could be a very interesting alternative to pyranose oxidase for applications in biotechnology or biofuel cells, electrical wiring of the enzyme bound to graphite rod electrodes is studied | Agaricus xanthodermus |
biotechnology | PDH is an alternative to pyranose oxidase for applications in biotechnology or biofuel cells, electrical wiring of the enzyme bound to graphite rod electrodes is studied | Leucoagaricus meleagris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-trehalose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.34 | - |
D-maltose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.35 | - |
sucrose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.44 | - |
D-glucose | at 0.1 M ethanolamine buffer at pH 9.4 | Agaricus xanthodermus | |
0.51 | - |
D-maltoheptaose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.62 | - |
D-mannose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.64 | - |
D-maltotriose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.64 | - |
D-maltopentaose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.65 | - |
D-glucose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.65 | - |
2-deoxy-D-glucose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
0.74 | - |
D-glucosamine | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.05 | - |
D-cellobiose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
5.84 | - |
D-xylose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
7.16 | - |
D-galactose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
8.63 | - |
L-arabinose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
10.84 | - |
beta-lactose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
12.14 | - |
2-deoxy-D-galactose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
12.31 | - |
L-fucose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Leucoagaricus meleagris | - |
- |
extracellular | - |
Agaricus xanthodermus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus xanthodermus | Q3L1D2 | - |
- |
Leucoagaricus meleagris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography | Leucoagaricus meleagris |
using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography | Agaricus xanthodermus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-galactose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
2-deoxy-D-glucose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
beta-lactose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-cellobiose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-galactose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-glucosamine + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-glucose + acceptor | - |
Leucoagaricus meleagris | 3-dehydro-D-glucose + reduced acceptor | - |
? | |
D-glucose + acceptor | - |
Agaricus xanthodermus | 3-dehydro-D-glucose + reduced acceptor | - |
? | |
D-maltoheptaose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-maltopentaose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-maltose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-maltotriose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-mannose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-trehalose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
D-xylose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
L-arabinose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
L-fucose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
sucrose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDH AM | - |
Leucoagaricus meleagris |
PDH AX | - |
Agaricus xanthodermus |
pyranose dehydrogenase | - |
Leucoagaricus meleagris |
pyranose dehydrogenase | - |
Agaricus xanthodermus |
pyranose:acceptor oxidoreductase | - |
Leucoagaricus meleagris |
pyranose:acceptor oxidoreductase | - |
Agaricus xanthodermus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM D-glucose, flow rate 1.0 mL/min | Leucoagaricus meleagris |
9 | - |
in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min | Agaricus xanthodermus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | in PBS, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min | Leucoagaricus meleagris |
7 | 10.5 | depending on the buffer, highest biosensor response in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min | Leucoagaricus meleagris |
8 | 10 | in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min | Agaricus xanthodermus |
8 | 9 | in Tris, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min | Leucoagaricus meleagris |