Literature summary for 1.1.99.28 extracted from

Lott, J.S.; Halbig, D.; Baker, H.M.; Hardman, M.J.; Sprenger, G.A.; Baker, E.N.
Crystal structure of a truncated mutant of glucose-fructose oxidoreductase shows that an N-terminal arm controls tetramer formation (2000), J. Mol. Biol., 304, 575-584.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
vapour diffusion in hanging drops, crystal structure of the NAD+ complex of a truncated form of the enzyme, GFORDELTA1-22/S64D, in which the first 22 residues of the N-terminal arm of the mature protein have been deleted, structure refined at 2.7 A resolution shows that the truncated form of the enzyme forms a dimer and implies that the N-terminal arm is essential for tetramer formation by wild-type GFOR	Zymomonas mobilis

Crystallization (Comment)

Organism

vapour diffusion in hanging drops, crystal structure of the NAD+ complex of a truncated form of the enzyme, GFORDELTA1-22/S64D, in which the first 22 residues of the N-terminal arm of the mature protein have been deleted, structure refined at 2.7 A resolution shows that the truncated form of the enzyme forms a dimer and implies that the N-terminal arm is essential for tetramer formation by wild-type GFOR

Zymomonas mobilis

Protein Variants

Protein Variants	Comment	Organism
DELTA1-22/S64D	S64D mutation converts the strict NADP+ spoecificity of wild-type GFOR to a dual NADP+/NAD+ specificity	Zymomonas mobilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Zymomonas mobilis	-	-

Organism

Organism	UniProt	Comment	Textmining
Zymomonas mobilis	Q07982	-	-

Subunits

Subunits	Comment	Organism
tetramer	the N-terminal arm is essential for tetramer formation by wild-type GFOR	Zymomonas mobilis

Synonyms

Synonyms	Comment	Organism
GFOR	-	Zymomonas mobilis
glucose-fructose oxidoreductase	-	Zymomonas mobilis

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	S64D mutation converts the strict NADP+ specificity of wild-type GFOR to a dual NADP+/NAD+ specificity	Zymomonas mobilis
NADP+	S64D mutation converts the strict NADP+ specificity of wild-type GFOR to a dual NADP+/NAD+ specificity	Zymomonas mobilis

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Release 2023.1 (January 2023)