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Literature summary for 1.1.99.28 extracted from
- Simultaneous enzymatic synthesis of gluconic acid and sorbitol. Production, purification, and application of glucose-fructose oxidoreductase and gluconolactonase (1997), Appl. Biochem. Biotechnol., 63-65, 173-188.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | activates | Zymomonas mobilis |  |
| Tween | activates | Zymomonas mobilis | |
General Stability
| General Stability | Organism |
|---|---|
| inactivation during substrate conversion | Zymomonas mobilis |
| thiol reagents stabilize. Dithiothreitol is the most efficient, 5-15 mM | Zymomonas mobilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Zymomonas mobilis | |
| NADP+ | - |
Zymomonas mobilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
glucose | - |
Zymomonas mobilis | |
| 400 | - |
fructose | - |
Zymomonas mobilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zymomonas mobilis | - |
strain DSM 473 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zymomonas mobilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 245 | 310 | - |
Zymomonas mobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Deoxy-D-glucose + D-fructose | at 16% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| D-glucose + D-fructose | - |
Zymomonas mobilis | D-glucono-1,5-lactone + D-sorbitol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Zymomonas mobilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
stable for 2 weeks | Zymomonas mobilis |
| 45 | - |
half-life: 55 h | Zymomonas mobilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Zymomonas mobilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.8 | 30°C, pH does not affect the stability | Zymomonas mobilis |
| 7 | - |
40°C, fast inactivation above | Zymomonas mobilis |
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