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Literature summary for 1.1.99.22 extracted from
- Enzymatic redox cofactor regeneration in organic media: functionalization and application of glycerol dehydrogenase and soluble transhydrogenase in reverse micelles (2005), Biotechnol. Prog., 21, 1192-1197.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 as His-tag fusion protein | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | 85% activation at 10 mM | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli JM109 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by Ni-affinity chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + NAD+ | - |
Escherichia coli | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Escherichia coli JM109 | dihydroxyacetone + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GLD | - |
Escherichia coli |
| glycerol dehydrogenase | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
in an aqueous buffer system | Escherichia coli |
| 10.5 | - |
in reverse micelles | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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