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Literature summary for 1.1.99.20 extracted from
- The first step in polyethylene glycol degradation by Sphingomonads proceeds via a flavoprotein alcohol dehydrogenase containing flavin adenine dinucleotide (2001), J. Bacteriol., 183, 6694-6698.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cloned, sequenced and expressed in Escherichia coli | Sphingopyxis terrae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
1-Heptanol | - |
Sphingopyxis terrae |  |
| 0.5 | - |
1-Hexanol | - |
Sphingopyxis terrae | |
| 0.6 | - |
benzyl alcohol | - |
Sphingopyxis terrae | |
| 0.7 | - |
PEG-400 | - |
Sphingopyxis terrae | |
| 0.8 | - |
PEG-300 | - |
Sphingopyxis terrae | |
| 1.6 | - |
PEG-1000 | - |
Sphingopyxis terrae | |
| 2.4 | - |
PEG-6000 | - |
Sphingopyxis terrae | |
| 4.2 | - |
tetraethylene glycol-monoethyl ether | - |
Sphingopyxis terrae | |
| 6.6 | - |
1-propanol | - |
Sphingopyxis terrae | |
| 14 | - |
Tetraethylene glycol | - |
Sphingopyxis terrae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Sphingopyxis terrae | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58800 | - |
2 * 58800, SDS-PAGE | Sphingopyxis terrae |
| 120000 | - |
gel filtration | Sphingopyxis terrae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingopyxis terrae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from a symbiotic mixed culture E-1, consisting of Sphingomonas terrae and a Rhizobium sp. | Sphingopyxis terrae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-heptanol + acceptor | - |
Sphingopyxis terrae | n-heptanal + reduced acceptor | - |
? | |
| 1-hexanol + acceptor | - |
Sphingopyxis terrae | n-hexanal + reduced acceptor | - |
? | |
| 1-propanol + 2,6-dichloro-phenolindophenol | - |
Sphingopyxis terrae | propanal + reduced 2,6-dichloro-phenolindophenol | - |
r | |
| aromatic alcohol + acceptor | - |
Sphingopyxis terrae | aromatic aldehyde + reduced acceptor | - |
? | |
| benzyl alcohol + acceptor | benzyl alcohol is the best substrate | Sphingopyxis terrae | benzaldehyde + reduced acceptor | - |
? | |
| PEG-1000 + acceptor | - |
Sphingopyxis terrae | ? | - |
? | |
| PEG-300 + acceptor | - |
Sphingopyxis terrae | ? | - |
? | |
| PEG-400 + acceptor | - |
Sphingopyxis terrae | ? | - |
? | |
| PEG-6000 + acceptor | - |
Sphingopyxis terrae | ? | - |
? | |
| polyethylene glycol + acceptor | - |
Sphingopyxis terrae | ? | - |
? | |
| primary alcohol + acceptor | - |
Sphingopyxis terrae | aldehyde + reduced acceptor | - |
? | |
| tetraethylene glycol + acceptor | - |
Sphingopyxis terrae | tetraethylene glycol aldehyde + reduced acceptor | - |
? | |
| tetraethyleneglycol monoethyl ether | - |
Sphingopyxis terrae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 58800, SDS-PAGE | Sphingopyxis terrae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Sphingopyxis terrae | |
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Release 2023.1 (January 2023)
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