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Literature summary for 1.1.99.18 extracted from

  • Langston, J.A.; Brown, K.; Xu, F.; Borch, K.; Garner, A.; Sweeney, M.D.
    Cloning, expression, and characterization of a cellobiose dehydrogenase from Thielavia terrestris induced under cellulose growth conditions (2012), Biochim. Biophys. Acta, 1824, 802-812 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae Thermothielavioides terrestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
cellobiose pH 6.0, 25°C Thermothielavioides terrestris
0.0056
-
cellotetraose pH 6.0, 25°C Thermothielavioides terrestris
0.0075
-
cellopentaose pH 6.0, 25°C Thermothielavioides terrestris
0.0082
-
cellotriose pH 6.0, 25°C Thermothielavioides terrestris
0.023
-
lactose pH 6.0, 25°C Thermothielavioides terrestris
0.078
-
2,6-dichlorophenol indophenol pH 6.0, 25°C Thermothielavioides terrestris

Organism

Organism UniProt Comment Textmining
Thermothielavioides terrestris G2RB43
-
-
Thermothielavioides terrestris ATCC 38088 G2RB43
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein about 40% carbohydrate content Thermothielavioides terrestris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.85
-
pH 6.0, 25°C Thermothielavioides terrestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellobiose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris ATCC 38088 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellopentaose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellopentaose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris ATCC 38088 cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellotetraose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris cellotetraono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellotetraose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris ATCC 38088 cellotetraono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellotriose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris cellotriono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellotriose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris ATCC 38088 cellotriono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
lactose + 2,6-dichlorophenol indophenol
-
Thermothielavioides terrestris 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
additional information no electron acceptor: oxygen. Very poor substrates: maltose, glucose Thermothielavioides terrestris ?
-
?
additional information no electron acceptor: oxygen. Very poor substrates: maltose, glucose Thermothielavioides terrestris ATCC 38088 ?
-
?

Subunits

Subunits Comment Organism
? x * 125000, SDS-PAGE, x * 90000, deglycosylated protein, x * 85600, calculated Thermothielavioides terrestris

Synonyms

Synonyms Comment Organism
cbdA
-
Thermothielavioides terrestris
Cdh
-
Thermothielavioides terrestris
Thite_59724
-
Thermothielavioides terrestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80.2
-
melting temperature Thermothielavioides terrestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.23
-
cellobiose pH 6.0, 25°C Thermothielavioides terrestris
1.23
-
cellotetraose pH 6.0, 25°C Thermothielavioides terrestris
1.23
-
cellopentaose pH 6.0, 25°C Thermothielavioides terrestris
1.25
-
cellotriose pH 6.0, 25°C Thermothielavioides terrestris
1.4
-
lactose pH 6.0, 25°C Thermothielavioides terrestris
2
-
2,6-dichlorophenol indophenol pH 6.0, 25°C Thermothielavioides terrestris

Cofactor

Cofactor Comment Organism Structure
flavin protein displays absorption maximum at 419 nm with a broad tail from about 450-480 nm, corresponding to the ferric heme and flavin groups respectively Thermothielavioides terrestris
heme protein displays absorption maximum at 419 nm with a broad tail from about 450-480 nm, corresponding to the ferric heme and flavin groups respectively Thermothielavioides terrestris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
62
-
lactose pH 6.0, 25°C Thermothielavioides terrestris
150
-
cellotriose pH 6.0, 25°C Thermothielavioides terrestris
160
-
cellopentaose pH 6.0, 25°C Thermothielavioides terrestris
220
-
cellotetraose pH 6.0, 25°C Thermothielavioides terrestris
3230
-
cellobiose pH 6.0, 25°C Thermothielavioides terrestris