KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
cellobiose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
0.125 | - |
cellobiose | at pH 5.0 and 30°C | Dichomera saubinetii | |
0.14 | - |
cellobiose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
0.16 | - |
cellobiose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
0.16 | - |
cellobiose | at pH 5.5 and 30°C | Neurospora crassa | |
0.17 | - |
cellobiose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
0.185 | - |
lactose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
0.2 | - |
lactose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
0.215 | - |
lactose | at pH 5.0 and 30°C | Dichomera saubinetii | |
0.217 | - |
lactose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
0.23 | - |
lactose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
0.325 | - |
lactose | at pH 5.5 and 30°C | Neurospora crassa | |
2.2 | - |
maltose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
8 | - |
maltose | at pH 5.0 and 30°C | Dichomera saubinetii | |
15.5 | - |
maltose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
18 | - |
maltose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
50 | - |
maltose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
71 | - |
D-glucose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
79 | - |
D-glucose | at pH 5.0 and 30°C | Dichomera saubinetii | |
87 | - |
D-glucose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
120 | - |
D-glucose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
120 | - |
maltose | at pH 5.5 and 30°C | Neurospora crassa | |
4000 | - |
D-glucose | at pH 5.´5 and 30°C | Neurospora crassa | |
17000 | - |
D-glucose | at pH 5.´5 and 30°C | Stachybotrys bisbyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81840 | - |
x * 81840, calculated from amino acid sequence | Thermothelomyces fergusii |
83587 | - |
x * 83587, calculated from amino acid sequence | Neurospora crassa |
83901 | - |
x * 83901, calculated from amino acid sequence | Hypoxylon haematostroma |
85000 | - |
x * 85000, SDS-PAGE | Thermothelomyces fergusii |
85000 | - |
x * 85000, SDS-PAGE | Hypoxylon haematostroma |
85942 | - |
x * 85942, calculated from amino acid sequence | Amesia atrobrunnea |
86688 | - |
x * 86688, calculated from amino acid sequence | Stachybotrys bisbyi |
86721 | - |
x * 86721, calculated from amino acid sequence | Dichomera saubinetii |
90000 | - |
x * 90000, SDS-PAGE | Neurospora crassa |
90000 | - |
x * 90000, SDS-PAGE | Amesia atrobrunnea |
100000 | - |
x * 100000, SDS-PAGE | Stachybotrys bisbyi |
115000 | - |
x * 115000, SDS-PAGE | Dichomera saubinetii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amesia atrobrunnea | E7D6C0 | - |
- |
Dichomera saubinetii | E7D6C1 | - |
- |
Hypoxylon haematostroma | - |
- |
- |
Neurospora crassa | - |
- |
- |
Stachybotrys bisbyi | E7D6C4 | - |
- |
Thermothelomyces fergusii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and phenyl-Source column chromatography | Neurospora crassa |
DEAE-Sepharose column chromatography and phenyl-Source column chromatography | Thermothelomyces fergusii |
DEAE-Sepharose column chromatography and phenyl-Source column chromatography | Stachybotrys bisbyi |
DEAE-Sepharose column chromatography and phenyl-Source column chromatography | Hypoxylon haematostroma |
DEAE-Sepharose column chromatography and phenyl-Source column chromatography | Amesia atrobrunnea |
DEAE-Sepharose column chromatography and phenyl-Source column chromatography | Dichomera saubinetii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.2 | - |
after 14.8fold purification, at pH 4.0 and 37°C | Stachybotrys bisbyi |
2.7 | - |
after 14.8fold purification, at pH 4.5 and 37°C | Stachybotrys bisbyi |
5.7 | - |
after 48.1fold purification, at pH 4.0 and 37°C | Neurospora crassa |
7.1 | - |
after 293fold purification, at pH 4.0 and 37°C | Amesia atrobrunnea |
7.4 | - |
after 21.2fold purification, at pH 4.0 and 37°C | Hypoxylon haematostroma |
8.9 | - |
after 7.3fold purification, at pH 4.0 and 37°C | Thermothelomyces fergusii |
9 | - |
after 21.2fold purification, at pH 5.0 and 37°C | Hypoxylon haematostroma |
10.3 | - |
after 48.1fold purification, at pH 5.5 and 37°C | Neurospora crassa |
11.5 | - |
after 293fold purification, at pH 6.0 and 37°C | Amesia atrobrunnea |
12.3 | - |
after 26.3fold purification, at pH 4.0 and 37°C | Dichomera saubinetii |
13.2 | - |
after 26.3fold purification, at pH 5.5 and 37°C | Dichomera saubinetii |
17.8 | - |
after 7.3fold purification, at pH 5.0 and 37°C | Thermothelomyces fergusii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichloroindophenol | - |
Neurospora crassa | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
cellobiose + 2,6-dichloroindophenol | - |
Thermothelomyces fergusii | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
cellobiose + 2,6-dichloroindophenol | - |
Stachybotrys bisbyi | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
cellobiose + 2,6-dichloroindophenol | - |
Hypoxylon haematostroma | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
cellobiose + 2,6-dichloroindophenol | - |
Amesia atrobrunnea | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
cellobiose + 2,6-dichloroindophenol | - |
Dichomera saubinetii | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Neurospora crassa | D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Thermothelomyces fergusii | D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Hypoxylon haematostroma | D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Amesia atrobrunnea | D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Dichomera saubinetii | D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Stachybotrys bisbyi | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + 2,6-dichloroindophenol | - |
Neurospora crassa | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
lactose + 2,6-dichloroindophenol | - |
Thermothelomyces fergusii | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
lactose + 2,6-dichloroindophenol | - |
Stachybotrys bisbyi | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
lactose + 2,6-dichloroindophenol | - |
Amesia atrobrunnea | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
lactose + 2,6-dichloroindophenol | - |
Dichomera saubinetii | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
lactose + 2,6-dichloroindophenol | - |
Hypoxylon haematostroma | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6dichloro-indophenol | - |
? | |
lactose + cytochrome c | - |
Neurospora crassa | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | - |
Thermothelomyces fergusii | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | - |
Stachybotrys bisbyi | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | - |
Hypoxylon haematostroma | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | - |
Amesia atrobrunnea | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | - |
Dichomera saubinetii | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | - |
Neurospora crassa | 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | - |
Thermothelomyces fergusii | 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | - |
Stachybotrys bisbyi | 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | - |
Hypoxylon haematostroma | 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | - |
Amesia atrobrunnea | 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | - |
Dichomera saubinetii | 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Neurospora crassa |
? | x * 90000, SDS-PAGE | Amesia atrobrunnea |
? | x * 100000, SDS-PAGE | Stachybotrys bisbyi |
? | x * 115000, SDS-PAGE | Dichomera saubinetii |
? | x * 85000, SDS-PAGE | Thermothelomyces fergusii |
? | x * 85000, SDS-PAGE | Hypoxylon haematostroma |
? | x * 81840, calculated from amino acid sequence | Thermothelomyces fergusii |
? | x * 83587, calculated from amino acid sequence | Neurospora crassa |
? | x * 83901, calculated from amino acid sequence | Hypoxylon haematostroma |
? | x * 85942, calculated from amino acid sequence | Amesia atrobrunnea |
? | x * 86688, calculated from amino acid sequence | Stachybotrys bisbyi |
? | x * 86721, calculated from amino acid sequence | Dichomera saubinetii |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Neurospora crassa |
Cdh | - |
Thermothelomyces fergusii |
Cdh | - |
Stachybotrys bisbyi |
Cdh | - |
Hypoxylon haematostroma |
Cdh | - |
Amesia atrobrunnea |
Cdh | - |
Dichomera saubinetii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
maltose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
0.6 | - |
maltose | at pH 5.5 and 30°C | Neurospora crassa | |
1.38 | - |
maltose | at pH 5.0 and 30°C | Dichomera saubinetii | |
1.45 | - |
maltose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
1.8 | - |
maltose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
4 | - |
maltose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
13 | - |
cellobiose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
16.8 | - |
D-glucose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
18.4 | - |
D-glucose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
19.9 | - |
cellobiose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
20.1 | - |
cellobiose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
20.2 | - |
lactose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
21.8 | - |
cellobiose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
23.6 | - |
D-glucose | at pH 5.0 and 30°C | Dichomera saubinetii | |
24.2 | - |
D-glucose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
24.7 | - |
lactose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
25 | - |
lactose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
25.6 | - |
D-glucose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
27.8 | - |
cellobiose | at pH 5.0 and 30°C | Dichomera saubinetii | |
28.9 | - |
lactose | at pH 5.0 and 30°C | Dichomera saubinetii | |
33.7 | - |
lactose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
37.7 | - |
D-glucose | at pH 5.5 and 30°C | Neurospora crassa | |
51.8 | - |
lactose | at pH 5.5 and 30°C | Neurospora crassa | |
53.1 | - |
cellobiose | at pH 5.5 and 30°C | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Stachybotrys bisbyi |
5 | - |
- |
Thermothelomyces fergusii |
5 | - |
- |
Hypoxylon haematostroma |
5 | - |
- |
Dichomera saubinetii |
5.5 | - |
- |
Neurospora crassa |
6 | - |
- |
Amesia atrobrunnea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermothelomyces fergusii | isoelectric focusing | - |
3.8 |
Amesia atrobrunnea | isoelectric focusing | - |
4.1 |
Dichomera saubinetii | isoelectric focusing | - |
4.2 |
Stachybotrys bisbyi | isoelectric focusing | - |
4.3 |
Hypoxylon haematostroma | isoelectric focusing | - |
4.3 |
Neurospora crassa | isoelectric focusing | - |
4.4 |
Thermothelomyces fergusii | calculated from amino acid sequence | - |
4.6 |
Amesia atrobrunnea | calculated from amino acid sequence | - |
5 |
Dichomera saubinetii | calculated from amino acid sequence | - |
5 |
Stachybotrys bisbyi | calculated from amino acid sequence | - |
5.1 |
Hypoxylon haematostroma | calculated from amino acid sequence | - |
5.7 |
Neurospora crassa | calculated from amino acid sequence | - |
6.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
maltose | at pH 5.5 and 30°C | Neurospora crassa | |
0.009 | - |
D-glucose | at pH 5.5 and 30°C | Neurospora crassa | |
0.01 | - |
maltose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
0.014 | - |
D-glucose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
0.08 | - |
maltose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
0.175 | - |
maltose | at pH 5.0 and 30°C | Dichomera saubinetii | |
0.195 | - |
D-glucose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
0.215 | - |
D-glucose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
0.26 | - |
maltose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
0.26 | - |
D-glucose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
0.3 | - |
D-glucose | at pH 5.0 and 30°C | Dichomera saubinetii | |
0.82 | - |
maltose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
93 | - |
cellobiose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
109 | - |
lactose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
109 | - |
lactose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
117 | - |
cellobiose | at pH 5.5 and 30°C | Stachybotrys bisbyi | |
124 | - |
lactose | at pH 5.0 and 30°C | Thermothelomyces fergusii | |
134 | - |
lactose | at pH 5.0 and 30°C | Dichomera saubinetii | |
136 | - |
cellobiose | at pH 5.0 and 30°C | Hypoxylon haematostroma | |
155 | - |
lactose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
159 | - |
lactose | at pH 5.5 and 30°C | Neurospora crassa | |
222 | - |
cellobiose | at pH 5.0 and 30°C | Dichomera saubinetii | |
279 | - |
cellobiose | at pH 6.0 and 30°C | Amesia atrobrunnea | |
332 | - |
cellobiose | at pH 5.5 and 30°C | Neurospora crassa |