Cloned (Comment) | Organism |
---|---|
wild type and mutant cellobiose dehydrogenase genes are cloned for heterologously expression in yeast Pichia pastoris | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
F166Y | the redox potential of heme in the mutant is lower than that of the wild type enzyme | Phanerodontia chrysosporium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | substrate inhibition | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00067 | - |
cytochrome c | mutant F166Y, cytochrome c reduction | Phanerodontia chrysosporium | |
0.00146 | - |
cytochrome c | wild type, cytochrome c reduction | Phanerodontia chrysosporium | |
0.0179 | - |
cytochrome c | mutant F166Y, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
0.0286 | - |
cytochrome c | wild type, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
0.056 | - |
ubiquinone | mutant F166Y, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
0.0579 | - |
ubiquinone | wild type, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
0.293 | - |
ubiquinone | mutant F166Y, ubiquinone reduction | Phanerodontia chrysosporium | |
0.326 | - |
ubiquinone | wild type, ubiquinone reduction | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + acceptor | Phanerodontia chrysosporium | acceptor: cytochrome c | cellobiono-1,5-lactone + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | Q01738 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + acceptor | acceptor: cytochrome c | Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
cellobiose + ubiquinone | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced ubiquinone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Phanerodontia chrysosporium |
cellobiose dehydrogenase | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
activity assay | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Phanerodontia chrysosporium | |
heme | b-type | Phanerodontia chrysosporium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.233 | - |
cytochrome c | mutant F166Y, cytochrome c reduction | Phanerodontia chrysosporium | |
2.51 | - |
cytochrome c | wild type, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
3.54 | - |
cytochrome c | mutant F166Y, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium |