Literature summary for 1.1.99.18 extracted from

Igarashi, K.; Yoshida, M.; Matsumura, H.; Nakamura, N.; Ohno, H.; Samejima, M.; Nishino, T.
Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium (2005), FEBS J., 272, 2869-2877.

Search for more literature for 1.1.99.18

Cloned(Commentary)

Cloned (Comment)	Organism
wild type and mutant cellobiose dehydrogenase genes are cloned for heterologously expression in yeast Pichia pastoris	Phanerodontia chrysosporium

Protein Variants

Protein Variants	Comment	Organism
F166Y	the redox potential of heme in the mutant is lower than that of the wild type enzyme	Phanerodontia chrysosporium

Inhibitors

Inhibitors	Comment	Organism	Structure
cytochrome c	substrate inhibition	Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00067	-	cytochrome c	mutant F166Y, cytochrome c reduction	Phanerodontia chrysosporium
0.00146	-	cytochrome c	wild type, cytochrome c reduction	Phanerodontia chrysosporium
0.0179	-	cytochrome c	mutant F166Y, cellobiose oxidation with electron acceptors	Phanerodontia chrysosporium
0.0286	-	cytochrome c	wild type, cellobiose oxidation with electron acceptors	Phanerodontia chrysosporium
0.056	-	ubiquinone	mutant F166Y, cellobiose oxidation with electron acceptors	Phanerodontia chrysosporium
0.0579	-	ubiquinone	wild type, cellobiose oxidation with electron acceptors	Phanerodontia chrysosporium
0.293	-	ubiquinone	mutant F166Y, ubiquinone reduction	Phanerodontia chrysosporium
0.326	-	ubiquinone	wild type, ubiquinone reduction	Phanerodontia chrysosporium

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Phanerodontia chrysosporium	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cellobiose + acceptor	Phanerodontia chrysosporium	acceptor: cytochrome c	cellobiono-1,5-lactone + reduced acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Phanerodontia chrysosporium	Q01738	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellobiose + acceptor	acceptor: cytochrome c	Phanerodontia chrysosporium	cellobiono-1,5-lactone + reduced acceptor	-	?
cellobiose + ubiquinone	-	Phanerodontia chrysosporium	cellobiono-1,5-lactone + reduced ubiquinone	-	?

Synonyms

Synonyms	Comment	Organism
Cdh	-	Phanerodontia chrysosporium
cellobiose dehydrogenase	-	Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	activity assay	Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	activity assay	Phanerodontia chrysosporium

Cofactor

Cofactor	Comment	Organism	Structure
flavin	-	Phanerodontia chrysosporium
heme	b-type	Phanerodontia chrysosporium

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.233	-	cytochrome c	mutant F166Y, cytochrome c reduction	Phanerodontia chrysosporium
2.51	-	cytochrome c	wild type, cellobiose oxidation with electron acceptors	Phanerodontia chrysosporium
3.54	-	cytochrome c	mutant F166Y, cellobiose oxidation with electron acceptors	Phanerodontia chrysosporium

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)