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Literature summary for 1.1.99.18 extracted from

  • Stapleton, P.C.; O'Brien, M.M.; O'Callaghan, J.; Dobson, A.D.W.
    Molecular cloning of the cellobiose dehydrogenase gene from Trametes versicolor and expression in Pichia pastoris (2004), Enzyme Microb. Technol., 34, 55-63.
No PubMed abstract available
Search for more literature for 1.1.99.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris under the control of the AOX1 methanol promoter, without affecting its kinetic characteristics Trametes versicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 cytochrome c pH 4.5, 25°C, wild-type enzyme Trametes versicolor
0.018
-
 cytochrome c pH 4.5, 25°C, recombinant enzyme Trametes versicolor
0.108
-
 cellobiose pH 4.5, 25°C, recombinant enzyme Trametes versicolor
0.128
-
 cellobiose pH 4.5, 25°C, wild-type enzyme Trametes versicolor
0.938
-
 lactose pH 4.5, 25°C, recombinant enzyme Trametes versicolor
0.95
-
 lactose pH 4.5, 25°C, wild-type enzyme Trametes versicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 x * 98000, SDS-PAGE Trametes versicolor

Organism

Organism UniProt Comment Textmining
Trametes versicolor Q875J3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the wild-type enzyme contains approximately 19% carbohydrate Trametes versicolor

Purification (Commentary)

Purification (Comment) Organism
-
 Trametes versicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Trametes versicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichloroindophenol
-
 Trametes versicolor cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
cellobiose + 3,5-di-tert-butylbenzoquinone
-
 Trametes versicolor cellobiono-1,5-lactone + reduced 3,5-di-tert-butylbenzoquinone
-
 ?
cellobiose + cytochrome c
-
 Trametes versicolor cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes versicolor 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?

Subunits

Subunits Comment Organism
? x * 98000, SDS-PAGE Trametes versicolor

Synonyms

Synonyms Comment Organism
Cdh
-
 Trametes versicolor
cellobiose dehydrogenase
-
 Trametes versicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 reaction with cellobiose and cytochrome c or 3,5-di-tert-butylbenzoquinone Trametes versicolor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 70% of maximal activity, 50°C: about 80% of maximal activity, 60°C: 30% of maximal activity with wild-type enzyme, 25% of maximal activity with recombinant enzyme, reaction with cellobiose and cytochrome c or 3,5-di-tert-butylbenzoquinone Trametes versicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 240 min, about 20% loss of activity of wild-type enzyme, about 30% loss of activity of recombinant enzyme Trametes versicolor
50
-
 60 min, about 55% loss of activity of wild-type enzyme and recombinant enzyme Trametes versicolor
55
-
 20 min, about 60% loss of activity of wild-type enzyme and recombinant enzyme Trametes versicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 two activity peaks, one at pH 3.5 and another at pH 4.5-4.75, the activity at pH 3.5 is 90% of the activity at pH 4.5-7.5, reaction with cellobiose and cytochrome c Trametes versicolor
4
-
 two activity peaks, one at pH 4 and another at pH 5, reaction with cellobiose and 3,5-di-tert-butylbenzoquinone Trametes versicolor
4.5 7.5 two activity peaks, one at pH 3.5 and another at pH 4.5-4.75, the activity at pH 3.5 is 90% of the activity at pH 4.5-7.5, reaction with cellobiose and cytochrome c Trametes versicolor
5
-
 two activity peaks, one at pH 4 and another at pH 5, reaction with cellobiose and 3,5-di-tert-butylbenzoquinone Trametes versicolor

pH Range

pH Minimum pH Maximum Comment Organism
3.2 5.3 pH 3.2: about 50% of maximal activity, pH 5.3: about 45% of maximal activity, reaction with cellobiose and cytochrome c Trametes versicolor