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Literature summary for 1.1.99.18 extracted from

  • Schou, C.; Christensen, M.H.; Schulein, M.
    Characterization of a cellobiose dehydrogenase from Humicola insolens (1998), Biochem. J., 330, 565-571.
No PubMed abstract available
Search for more literature for 1.1.99.18

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 cellobiose
-
 Humicola insolens
0.012
-
 ferricyanide
-
 Humicola insolens
0.017
-
 cellopentaose
-
 Humicola insolens
0.019
-
 cellotriose
-
 Humicola insolens
0.021
-
 cellotetraose
-
 Humicola insolens
0.026
-
 2,6-dichlorphenolindophenol
-
 Humicola insolens
0.051
-
 lactose
-
 Humicola insolens
0.093
-
 cytochrome c
-
 Humicola insolens
0.132
-
 benzoquinone
-
 Humicola insolens
7.1
-
 xylobiose
-
 Humicola insolens
11
-
 maltose
-
 Humicola insolens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Humicola insolens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 gel filtration Humicola insolens

Organism

Organism UniProt Comment Textmining
Humicola insolens
-
 soft-rot thermophilic fungus
-

Purification (Commentary)

Purification (Comment) Organism
arginine sepharose, S-sepharose, HPQ-sepharose, Superdex Humicola insolens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4409
-
-
 Humicola insolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenolindophenol
-
 Humicola insolens cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 r
cellobiose + acceptor electron acceptors: 2,6-dichlorophenolindophenol, benzoquinone, ferricyanide, cytochrome c, methylene blue, O2 Humicola insolens cellobiono-1,5-lactone + reduced acceptor
-
 ?
cellopentaose + acceptor
-
 Humicola insolens cellopentaono-1,5-lactone + reduced acceptor
-
 ?
cellotetraose + acceptor
-
 Humicola insolens cellotetrono-1,5-lactone + reduced acceptor
-
 ?
cellotriose + acceptor
-
 Humicola insolens cellotriono-1,5-lactone + reduced acceptor
-
 ?
lactose + acceptor
-
 Humicola insolens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
 ?
maltose + acceptor
-
 Humicola insolens 4-O-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
 ?
xylobiose + acceptor
-
 Humicola insolens 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 92000, SDS-PAGE Humicola insolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 sharp drop above Humicola insolens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.118
-
 xylobiose
-
 Humicola insolens
1.2
-
 maltose
-
 Humicola insolens
3
-
 xylobiose
-
 Humicola insolens
12
-
 cellotriose
-
 Humicola insolens
12
-
 cellotetraose
-
 Humicola insolens
12
-
 cellopentaose
-
 Humicola insolens
14
-
 cellobiose
-
 Humicola insolens
14
-
 lactose
-
 Humicola insolens
14
-
 ferricyanide
-
 Humicola insolens
17
-
 2,6-dichlorphenolindophenol
-
 Humicola insolens
21
-
 benzoquinone
-
 Humicola insolens
27
-
 cytochrome c
-
 Humicola insolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 methylen blue as electron acceptor Humicola insolens
7
-
 2,6-dichlorophenolindophenol as electron acceptor Humicola insolens
7
-
 3,5-di-tert-butyl-1,2-benzoquinone as electron acceptor Humicola insolens
7 9 cytochrome c as electron acceptor Humicola insolens
9
-
 ferricyanide as electron acceptor Humicola insolens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Humicola insolens
heme b
-
 Humicola insolens