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Literature summary for 1.1.99.1 extracted from
- Characterization of choline dehydrogenase from Pseudomonas aeruginosa A-16 (1976), Agric. Biol. Chem., 40, 2077-2084.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 1 mM causes 100% inhibition | Pseudomonas aeruginosa |  |
| Benzyltrimethylammonium chloride | slight | Pseudomonas aeruginosa | |
| Betaine hydrochloride | slight | Pseudomonas aeruginosa | |
| CuSO4 | 1 mM causes 100% inhibition | Pseudomonas aeruginosa | |
| dimethylglycine | slight | Pseudomonas aeruginosa | |
| DL-Carnitine hydrochloride | slight | Pseudomonas aeruginosa | |
| glycine | slight | Pseudomonas aeruginosa | |
| HgCl2 | 1 mM causes 100% inhibition | Pseudomonas aeruginosa | |
| iodoacetic acid | - |
Pseudomonas aeruginosa | |
| KCN | 1 mM inhibits | Pseudomonas aeruginosa | |
| Mercuric acetate | - |
Pseudomonas aeruginosa | |
| MnCl2 | 1 mM causes 100% inhibition | Pseudomonas aeruginosa | |
| p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
| Phenyltriethylammonium iodide | slight | Pseudomonas aeruginosa | |
| Tetra-n-butylammonium iodide | slight | Pseudomonas aeruginosa | |
| Tetra-n-propylammonium iodide | slight | Pseudomonas aeruginosa | |
| Tetraethylammonium iodide | slight | Pseudomonas aeruginosa | |
| Tetramethylammonium iodide | slight | Pseudomonas aeruginosa | |
| Triethylbenzylammonium iodide | slight | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
phenazine methosulfate | - |
Pseudomonas aeruginosa | |
| 1.7 | - |
choline | - |
Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | - |
Pseudomonas aeruginosa | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
A-16 strain | - |
| Pseudomonas aeruginosa A-16 | - |
A-16 strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using Triton-treatment, chloroform-treatment and column chromatography on Sepharose 6B | Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C , stable | Pseudomonas aeruginosa |
| 5°C, pH 7.4, complete loss of activity, partially purified enzyme, 5-6 days | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + acceptor | acceptor 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazolium bromide, particulate enzyme: 12.4% relative activity to phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | cytochrome c, particulate enzyme: 3.1% relative activity to phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor ferricyanide, particulate enzyme: 112% relative activity to phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor 2,6-dichlorophenolindophenol, particulate enzyme: 3.2% relative activity to phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor: phenazine methosulfate, 100% relative activity | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | electron acceptor: phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazolium bromide, particulate enzyme: 12.4% relative activity to phenazine methosulfate | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | cytochrome c, particulate enzyme: 3.1% relative activity to phenazine methosulfate | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor ferricyanide, particulate enzyme: 112% relative activity to phenazine methosulfate | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor 2,6-dichlorophenolindophenol, particulate enzyme: 3.2% relative activity to phenazine methosulfate | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor: phenazine methosulfate, 100% relative activity | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
| choline + phenazine methosulfate | - |
Pseudomonas aeruginosa | betaine aldehyde + reduced phenazine methosulfate | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
10 min, pH 8.3, 10% loss of activity | Pseudomonas aeruginosa |
| 40 | - |
10 min, pH 8.3, 50% loss of activity | Pseudomonas aeruginosa |
| 50 | - |
10 min, pH 8.3, 100% loss of activity | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10 | about 50% of activity maximum at pH 7.5 and pH 10.0 | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme requires no readily dissociable coenzyme for activity | Pseudomonas aeruginosa |
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