Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Clostridium perfringens |
Protein Variants | Comment | Organism |
---|---|---|
C15A/C19A/C22A | less stable than wild-type enzyme, loss of one of the three [4Fe-4S]2+ clusters | Clostridium perfringens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]2+ cluster | three [4Fe-4S]2+ clusters per enzyme molecule | Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45740 | - |
gel filtration | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q8XMQ3 | - |
- |
Clostridium perfringens type A | Q8XMQ3 | - |
- |
Storage Stability | Organism |
---|---|
-80°C, 50 mM potassium HEPES, pH 7.5, 500 mM KCl, 10 mM DTT, and 30% glycerol | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-YYTSPMSAPARSMLLTGN + S-adenosyl-L-methionine | a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite | Clostridium perfringens | Ac-YYTSPM-3-oxo-L-Ala-APARSMLLTGN + L-methionine + 5'-deoxyadenosine | - |
? | |
Ac-YYTSPMTAPARSMLLTGN + S-adenosyl-L-methionine | a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite | Clostridium perfringens | Ac-YYTSPM-(2S)-2-amino-3-oxobutanoyl-APARSMLLTGN + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O | - |
? | |
additional information | the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a serine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases. No reaction with Ac-YYTSPM(allo)TAPARSMLLTGN | Clostridium perfringens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1* 45740, deduced form the molecular weight of the enzyme/substrate complex | Clostridium perfringens |
Synonyms | Comment | Organism |
---|---|---|
anaerobic sulfatase-maturating enzyme | - |
Clostridium perfringens |
nSMEcpe | - |
Clostridium perfringens |
General Information | Comment | Organism |
---|---|---|
metabolism | involved in the pathway of sulfatase oxidation | Clostridium perfringens |