Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | S-adenosyl-L-methionine is directly reduced by the Fe-S center of the small subunits during the activation of the enzyme, resulting in methionine and glycyl radical formation. S-adenosyl-L-methionine binds to the small subunits with a Kd of 10 microM and a 1:1 stoichiometry. Dithiothreitol triggers the cleavage of S-adenosyl-L-methionine, leading glycyl radical formation. 3 methionines are formed per mol of protein | Escherichia coli | |
[4Fe-4S]-center | S-adenosyl-L-methionine is directly reduced by the Fe-S center of the small subunits during the activation of the enzyme | Escherichia coli |