Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K2SO4 | 100 mM, more than 90% inhibition | Methanoculleus thermophilus | |
KCl | 100 mM, more than 90% inhibition | Methanoculleus thermophilus | |
Na2SO4 | 100 mM, more than 90% inhibition | Methanoculleus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
propanone | pH 4.2, 40°C | Methanoculleus thermophilus | |
2.5 | - |
2-propanol | pH 4.2, 40°C | Methanoculleus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
2 * 39500, SDS-PAGE | Methanoculleus thermophilus |
68000 | - |
gel filtration | Methanoculleus thermophilus |
100000 | - |
ultrafiltration | Methanoculleus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanoculleus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanoculleus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
110 | - |
2-propanone, pH 4.2, 40°C | Methanoculleus thermophilus |
176 | - |
2-propanol, pH 4.2, 40°C | Methanoculleus thermophilus |
Storage Stability | Organism |
---|---|
4°C, almost no loss in specific activity after 1 year | Methanoculleus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-propanol + oxidized coenzyme F420 | - |
Methanoculleus thermophilus | propanone + reduced coenzyme F420 | propanone i.e. acetone | r | |
additional information | activity with ethanol or 1-propanol at the same concentration is only 0.1% compared to activity with 2-propanol. Acetaldehyde and propionaldehyde are reduced at the same rate as 2-propanone | Methanoculleus thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 39500, SDS-PAGE | Methanoculleus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
F420-specific sec-ADH | - |
Methanoculleus thermophilus |
F420-specific secondary alcohol dehydrogenase | - |
Methanoculleus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 5.8, the activity of a 20-fold dilution decreases to 5% within 20 min. If 100 mM potassium sulfate is present 40% of the activity is left after 20 min | Methanoculleus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
- |
Methanoculleus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | pH 3.0: about 30% of maximal activity, pH 7.0: about 30% of maximal activity | Methanoculleus thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
activity loss of a 50fold diluted extract tested at 0-28°C is least around pH 5.8 (30% in 23 days at 23°C) | Methanoculleus thermophilus |
Organism | Comment | Expression |
---|---|---|
Methanoculleus thermophilus | induced by its substrate, expression is faster if H2 and CO2 are present as energy source | up |