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Literature summary for 1.1.98.3 extracted from

  • Li, H.; Jogl, G.
    Crystal structure of decaprenylphosphoryl-beta-D-ribose 2'-epimerase from Mycobacterium smegmatis (2013), Proteins, 81, 538-543.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.98.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21star cells Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 15-20% (v/v) isopropanol, 0.2 M sodium citrate pH 6.8, and 0.5% (w/v) N,N-dimethyldodecylamine-N-oxide Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD Mycolicibacterium smegmatis
-
 trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2
-
 ?
trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD Mycolicibacterium smegmatis ATCC 700084
-
 trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0R607
-
-
Mycolicibacterium smegmatis ATCC 700084 A0R607
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-agarose affinity resin column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD
-
 Mycolicibacterium smegmatis trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2
-
 ?
trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD
-
 Mycolicibacterium smegmatis ATCC 700084 trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
decaprenylphosphoryl-beta-D-ribose 2'-epimerase
-
 Mycolicibacterium smegmatis
DprE1
-
 Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Mycolicibacterium smegmatis