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Literature summary for 1.1.7.1 extracted from

  • Johannes, J.; Unciuleac, M.C.; Friedrich, T.; Warkentin, E.; Ermler, U.; Boll, M.
    Inhibitors of the molybdenum cofactor containing 4-hydroxybenzoyl-CoA reductase (2008), Biochemistry, 47, 4964-4972.
    View publication on PubMed
Search for more literature for 1.1.7.1

Crystallization (Commentary)

Crystallization (Comment) Organism
the structures of dithionite- and azide-bound 4-HBCR are solved at 2.1 and 2.2 A, respectively Thauera aromatica

Inhibitors

Inhibitors Comment Organism Structure
azide previously reported, not confirmed Thauera aromatica
cyanide cyanide inhibition occurs in both the oxidized and reduced state of 4-HBCR, in the reduced state 4-HBCR is reactivated by simple oxidation, in the oxidized state reactivation is only achieved in the presence of sulfide Thauera aromatica
Dithionite reactivation by oxidation Thauera aromatica
titanium(III)citrate irreversible inhibition Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
-
 Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoyl-CoA + oxidized ferredoxin + H2O Thauera aromatica
-
 4-hydroxybenzoyl-CoA + reduced ferredoxin
-
 ?
benzoyl-CoA + oxidized ferredoxin + H2O Thauera aromatica K172
-
 4-hydroxybenzoyl-CoA + reduced ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Thauera aromatica
-
-
-
Thauera aromatica K172
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from extracts of Thauera aromatica, three chromatographic steps, using anion exchange chromatography on DEAE-Sepharose, Hi Load Q-Sepharose and chromatography on Cibacron Blue agarose Thauera aromatica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
 60 mg of enriched 4-HBCR is maximally obtained from 200 g of cells with specific activities of 3-5 micromol of 4-OH-BCoA reduced min-1 mg-1 Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-CoA + oxidized ferredoxin + H2O
-
 Thauera aromatica 4-hydroxybenzoyl-CoA + reduced ferredoxin
-
 ?
benzoyl-CoA + oxidized ferredoxin + H2O
-
 Thauera aromatica K172 4-hydroxybenzoyl-CoA + reduced ferredoxin
-
 ?

Subunits

Subunits Comment Organism
oligomer (alphabetagamma)2 subunit architecture Thauera aromatica

Synonyms

Synonyms Comment Organism
4-HBCR
-
 Thauera aromatica
4-hydroxybenzoyl-CoA reductase
-
 Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 activity assay Thauera aromatica

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Thauera aromatica
Ferredoxin
-
 Thauera aromatica
iron-sulfur centre
-
 Thauera aromatica
molybdopterin cytosine dinucleotide
-
 Thauera aromatica