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Literature summary for 1.1.5.B3 extracted from

  • Treu, B.L.; Minteer, S.D.
    Isolation and purification of PQQ-dependent lactate dehydrogenase from Gluconobacter and use for direct electron transfer at carbon and gold electrodes (2008), Bioelectrochemistry, 74, 73-77.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the PQQ-dependent lactate dehydrogenase from Gluconobacter is capable of direct electron transfer at carbon and gold electrodes, which makes it useful for application in biosensors or biofuel cells, overview Gluconobacter oxydans
biotechnology the PQQ-dependent lactate dehydrogenase from Gluconobacter is capable of direct electron transfer at carbon and gold electrodes, which makes it useful for application in biosensors or biofuel cells, overview Gluconobacter sp.

Protein Variants

Protein Variants Comment Organism
additional information bioanode fabrication using the partially purified enzyme immobilized on a membrane, usage of carbon fiber paper, overview Gluconobacter sp.

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
ATCC 621
-
Gluconobacter sp.
-
strain 33
-
Gluconobacter sp. DSM 3504 / ATCC 15163
-
strain 33
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from membranes by solubilization with Triton X-100 and anion exchange chromatography Gluconobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + 2,6-dichlorophenol indophenol reduction of the acceptor 2,6-dichlorophenol indophenol is mediated by phenazine methosulfate Gluconobacter oxydans pyruvate + reduced 2,6-dichlorophenol indophenol
-
?
L-lactate + 2,6-dichlorophenol indophenol reduction of the acceptor 2,6-dichlorophenol indophenol is mediated by phenazine methosulfate Gluconobacter sp. pyruvate + reduced 2,6-dichlorophenol indophenol
-
?
L-lactate + 2,6-dichlorophenol indophenol reduction of the acceptor 2,6-dichlorophenol indophenol is mediated by phenazine methosulfate Gluconobacter sp. DSM 3504 / ATCC 15163 pyruvate + reduced 2,6-dichlorophenol indophenol
-
?

Synonyms

Synonyms Comment Organism
PQQ-dependent lactate dehydrogenase
-
Gluconobacter oxydans
PQQ-dependent lactate dehydrogenase
-
Gluconobacter sp.
PQQ-LDH
-
Gluconobacter oxydans
PQQ-LDH
-
Gluconobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gluconobacter oxydans
37
-
assay at Gluconobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Gluconobacter oxydans
7.3
-
assay at Gluconobacter sp.

Cofactor

Cofactor Comment Organism Structure
2,6-dichlorophenol indophenol
-
Gluconobacter oxydans
2,6-dichlorophenol indophenol
-
Gluconobacter sp.
phenazine methosulfate mediates reduction of 2,6-dichlorophenol-indophenol Gluconobacter oxydans
phenazine methosulfate mediates reduction of 2,6-dichlorophenol-indophenol Gluconobacter sp.
pyrroloquinoline quinone dependent on Gluconobacter oxydans
pyrroloquinoline quinone dependent on Gluconobacter sp.