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Literature summary for 1.1.5.9 extracted from

  • Ozawa, K.; Iwasa, H.; Sasaki, N.; Kinoshita, N.; Hiratsuka, A.; Yokoyama, K.
    Identification and characterization of thermostable glucose dehydrogenases from thermophilic filamentous fungi (2017), Appl. Microbiol. Biotechnol., 101, 173-183 .
    View publication on PubMed
Search for more literature for 1.1.5.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Pichia pastoris Rasamsonia emersonii
expression in Escherichia coli and Pichia pastoris Thermoascus crustaceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 D-glucose pH 7.0, 37°C, glycosylated recombinant protein Thermoascus crustaceus
4.6
-
 D-glucose pH 7.0, 37°C, unglycosylated recombinant protein Thermoascus crustaceus
337
-
 D-glucose pH 7.0, 37°C, glycosylated recombinant protein Rasamsonia emersonii
406
-
 D-glucose pH 7.0, 37°C, unglycosylated recombinant protein Rasamsonia emersonii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular signal peptide consists of residues Met1 to Ala16 Thermoascus crustaceus
-
-
extracellular signal peptide consists of residues Met1 to Ala17 Rasamsonia emersonii
-
-

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii A0A1E1GL61
-
-
Thermoascus crustaceus A0A1E1GL87
-
-
Thermoascus crustaceus NBRC 9129 A0A1E1GL87
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein x * 80000-200000, recombinant protein produced in Pichia pastoris, SDS-PAGE. x * 58000, unglycosylated protein Thermoascus crustaceus
glycoprotein x * 90000-200000, recombinant protein produced in Pichia pastoris, SDS-PAGE. x * 65000, unglycosylated protein Rasamsonia emersonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 pH 7.0, 37°C, specific activity of unglycosylated recombinant protein is 16000 U/microM, specific activity of glycosylated recombinant protein is 13500 U/microM Thermoascus crustaceus
additional information
-
 pH 7.0, 37°C, specific activity of unglycosylated recombinant protein is 9210 U/microM, specific activity of glycosylated recombinant protein is 8790 U/microM Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol
-
 Rasamsonia emersonii D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol
-
 Thermoascus crustaceus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol
-
 Thermoascus crustaceus NBRC 9129 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
additional information no substrate: maltose Rasamsonia emersonii ?
-
 ?
additional information no substrate: maltose Thermoascus crustaceus ?
-
 ?
additional information no substrate: maltose Thermoascus crustaceus NBRC 9129 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80000-200000, recombinant protein produced in Pichia pastoris, SDS-PAGE. x * 58000, unglycosylated protein Thermoascus crustaceus
? x * 90000-200000, recombinant protein produced in Pichia pastoris, SDS-PAGE. x * 65000, unglycosylated protein Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
FADGH
-
 Rasamsonia emersonii
FADGH
-
 Thermoascus crustaceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 ungylcosylated recombinant protein Rasamsonia emersonii
60
-
 ungylcosylated recombinant protein Thermoascus crustaceus
65
-
 gylcosylated recombinant protein Rasamsonia emersonii
65
-
 gylcosylated recombinant protein Thermoascus crustaceus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 60 ungylcosylated recombinant protein Thermoascus crustaceus
55 60 ungylcosylated recombinant protein Rasamsonia emersonii
60 65 gylcosylated recombinant protein Thermoascus crustaceus
65 70 gylcosylated recombinant protein Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 15 min, no loss of activity Rasamsonia emersonii
45
-
 15 min, no loss of activity Thermoascus crustaceus
51.6
-
 melting temperature, unglycosylated recombinant protein Thermoascus crustaceus
52.7
-
 melting temperature, unglycosylated recombinant protein Rasamsonia emersonii
60
-
 15 min, unglycosylated recombinant protein, complete loss of activity, glycosylated recombinant protein, 75% residual activity Thermoascus crustaceus
60
-
 15 min, unglycosylated recombinant protein, complete loss of activity, glycosylated recombinant protein, 89% residual activity Rasamsonia emersonii
62.5
-
 melting temperature, glycosylated recombinant protein Thermoascus crustaceus
66.4
-
 melting temperature, glycosylated recombinant protein Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 both glycosylated and unglycosylated recombinant protein Thermoascus crustaceus
5
-
 glycosylated recombinant protein Rasamsonia emersonii
6
-
 unglycosylated recombinant protein Rasamsonia emersonii

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 9 glycosylated recombinant protein Rasamsonia emersonii
2 9 glycosylated recombinant protein Thermoascus crustaceus
3 8 unglycosylated recombinant protein Rasamsonia emersonii
3 8 unglycosylated recombinant protein Thermoascus crustaceus

Cofactor

Cofactor Comment Organism Structure
FAD purified protein shows major peaks of oxidized flavin at 380 and 450 nm Rasamsonia emersonii
FAD purified protein shows major peaks of oxidized flavin at 380 and 450 nm Thermoascus crustaceus