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Literature summary for 1.1.5.9 extracted from

  • Yang, Y.; Huang, L.; Wang, J.; Wang, X.; Xu, Z.
    Efficient expression, purification, and characterization of a novel FAD-dependent glucose dehydrogenase from Aspergillus terreus in Pichia pastoris (2014), J. Microbiol. Biotechnol., 24, 1516-1524.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
89.7
-
D-glucose at pH 7.5 and 55┬░C Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus Q0CDD9
-
-
Aspergillus terreus NIH2624 Q0CDD9
-
-

Purification (Commentary)

Purification (Comment) Organism
isopropanol precipitation and nickel affinity column chromatography Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + 2,6-dichlorophenolindophenol 0.5% activity compared to D-glucose Aspergillus terreus ? + reduced 2,6-dichlorophenolindophenol
-
?
D-galactose + 2,6-dichlorophenolindophenol 2.2% activity compared to D-glucose Aspergillus terreus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-galactose + 2,6-dichlorophenolindophenol 2.2% activity compared to D-glucose Aspergillus terreus NIH2624 D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Aspergillus terreus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Aspergillus terreus NIH2624 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-lactose + 2,6-dichlorophenolindophenol 0.6% activity compared to D-glucose Aspergillus terreus D-lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-lactose + 2,6-dichlorophenolindophenol 0.6% activity compared to D-glucose Aspergillus terreus NIH2624 D-lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol 7.4% activity compared to D-glucose Aspergillus terreus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol 7.4% activity compared to D-glucose Aspergillus terreus NIH2624 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
L-arabinose + 2,6-dichlorophenolindophenol 1.5% activity compared to D-glucose Aspergillus terreus L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + 2,6-dichlorophenolindophenol 17.7% activity compared to D-glucose Aspergillus terreus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
additional information no activity with D-fructose, S-sorbitol, and D-sucrose Aspergillus terreus ?
-
?
additional information no activity with D-fructose, S-sorbitol, and D-sucrose Aspergillus terreus NIH2624 ?
-
?

Subunits

Subunits Comment Organism
? x * 83000-150000, SDS-PAGE Aspergillus terreus

Synonyms

Synonyms Comment Organism
FAD-dependent glucose dehydrogenase
-
Aspergillus terreus
FAD-GDH
-
Aspergillus terreus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
55
-
-
Aspergillus terreus

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
30 65 about 70% activity at 30┬░C, about 80% activity at 35┬░C, about 90% activity at 45┬░C, about 95% activity at 50┬░C, 100% activity at 55┬░C, about 98% activity at 60┬░C, about 85% activity at 65┬░C Aspergillus terreus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
60
-
the enzyme displays very high thermal stability with a half-life of 82 min at 60┬░C Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Aspergillus terreus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 more than 70% activity between pH 6.0 and 8.5 Aspergillus terreus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 the enzyme remains stable after 20 min at pH 3.0-9.0 Aspergillus terreus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Aspergillus terreus