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Literature summary for 1.1.5.9 extracted from

  • Satake, R.; Ichiyanagi, A.; Ichikawa, K.; Hirokawa, K.; Araki, Y.; Yoshimura, T.; Gomi, K.
    Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, molecular cloning and gene expression in Aspergillus sojae (2015), J. Biosci. Bioeng., 120, 498-503.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus sojae Mucor circinelloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
28.3
-
D-glucose recombinant enzyme, apparent value, at pH 7.0 and 37┬░C Mucor circinelloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
-
Mucor circinelloides

Organism

Organism UniProt Comment Textmining
Mucor circinelloides
-
-
-
Mucor circinelloides NISL0103
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Mucor circinelloides

Purification (Commentary)

Purification (Comment) Organism
Toyopearl-butyl 650C column chromatography and SP-Sepharose column chromatography Mucor circinelloides

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Mucor circinelloides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + 2,6-dichlorophenolindophenol 0.44% activity compared to D-glucose Mucor circinelloides D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-galactose + 2,6-dichlorophenolindophenol 0.44% activity compared to D-glucose Mucor circinelloides NISL0103 D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol the enzyme has high specificity toward D-glucose Mucor circinelloides D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol the enzyme has high specificity toward D-glucose Mucor circinelloides NISL0103 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol 1.53% activity compared to D-glucose Mucor circinelloides D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol 1.53% activity compared to D-glucose Mucor circinelloides NISL0103 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + 2,6-dichlorophenol indophenol 1.09% activity compared to D-glucose Mucor circinelloides maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + 2,6-dichlorophenol indophenol 1.09% activity compared to D-glucose Mucor circinelloides NISL0103 maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltotetraose + 2,6-dichlorophenolindophenol 0.66% activity compared to D-glucose Mucor circinelloides maltotetraono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltotriose + 2,6-dichlorophenolindophenol 0.88% activity compared to D-glucose Mucor circinelloides maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
mannose + 2,6-dichlorophenolindophenol 0.66% activity compared to D-glucose Mucor circinelloides mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
additional information no activity with sucrose Mucor circinelloides ?
-
?
additional information no activity with sucrose Mucor circinelloides NISL0103 ?
-
?
trehalose + 2,6-dichlorophenolindophenol 0.22% activity compared to D-glucose Mucor circinelloides ? + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
? x * 69537, calculated from amino acid sequence Mucor circinelloides
? x * 90000-130000, SDS-PAGE Mucor circinelloides

Synonyms

Synonyms Comment Organism
FAD-dependent GDH
-
Mucor circinelloides
FAD-GDH
-
Mucor circinelloides

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
40
-
-
Mucor circinelloides

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
25 50 about 70% activity at 25┬░C, about 90% activity at 30┬░C, about 95% activity at 35┬░C, 100% activity at 40┬░C, about 65% activity at 45┬░C, less than 20% activity at 50┬░C Mucor circinelloides

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
25 40 the enzyme remains stable after 15 min at 25-40┬░C, then activity drops to about 70% at 50┬░C and is completely lost at 55┬░C Mucor circinelloides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
749.7
-
D-glucose recombinant enzyme, apparent value, at pH 7.0 and 37┬░C Mucor circinelloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5
-
Mucor circinelloides

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
approximately 80% of the enzymatic activity is retained after incubation at 40┬░C for 15 min at pH 5.0. Treatment at 25┬░C for 16 h shows that the enzyme is stable from pH 3.5 to 7.0 Mucor circinelloides

Cofactor

Cofactor Comment Organism Structure
FAD
-
Mucor circinelloides