Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BLR(DE3) and Pichia pastoris strain GS115 | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Thermoascus aurantiacus NBRC 6766 | - |
- |
- |
Thermoascus aurantiacus NBRC 9748 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | about 11% activity compared to D-glucose | Thermoascus aurantiacus | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | about 8.4% activity compared to D-glucose | Thermoascus aurantiacus | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | about 11% activity compared to D-glucose | Thermoascus aurantiacus NBRC 6766 | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | about 8.4% activity compared to D-glucose | Thermoascus aurantiacus NBRC 6766 | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | about 11% activity compared to D-glucose | Thermoascus aurantiacus NBRC 9748 | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | about 8.4% activity compared to D-glucose | Thermoascus aurantiacus NBRC 9748 | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | 100% activity | Thermoascus aurantiacus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | 100% activity | Thermoascus aurantiacus NBRC 6766 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | 100% activity | Thermoascus aurantiacus NBRC 9748 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-raffinose + 2,6-dichlorophenolindophenol | about 3.2% activity compared to D-glucose | Thermoascus aurantiacus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-raffinose + 2,6-dichlorophenolindophenol | about 4.5% activity compared to D-glucose | Thermoascus aurantiacus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | about 50% activity compared to D-glucose | Thermoascus aurantiacus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | about 40% activity compared to D-glucose | Thermoascus aurantiacus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | about 50% activity compared to D-glucose | Thermoascus aurantiacus NBRC 6766 | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | about 50% activity compared to D-glucose | Thermoascus aurantiacus NBRC 9748 | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + 2,6-dichlorophenolindophenol | about 11% activity compared to D-glucose | Thermoascus aurantiacus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + 2,6-dichlorophenolindophenol | about 21% activity compared to D-glucose | Thermoascus aurantiacus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose | Thermoascus aurantiacus | ? | - |
? | |
additional information | no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose | Thermoascus aurantiacus NBRC 6766 | ? | - |
? | |
additional information | no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose | Thermoascus aurantiacus NBRC 9748 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent glucose dehydrogenase | - |
Thermoascus aurantiacus |
FADGDH | - |
Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | after heat treatment at 40-60°C for 15 min, the enzyme maintains its activity close to 100% | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermoascus aurantiacus |
6 | 7 | - |
Thermoascus aurantiacus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | the enzyme is stable for 20 h between pH 3.0 and 11.0 | Thermoascus aurantiacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Thermoascus aurantiacus |