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Literature summary for 1.1.5.9 extracted from

  • Iwasa, H.; Ozawa, K.; Sasaki, N.; Kinoshita, N.; Hiratsuka, A.; Yokoyama, K.
    Thermostable FAD-dependent glucose dehydrogenases from thermophilic filamentous fungus Thermoascus aurantiacus (2016), Electrochemistry, 84, 342-348.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BLR(DE3) and Pichia pastoris strain GS115 Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
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-
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Thermoascus aurantiacus NBRC 6766
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-
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Thermoascus aurantiacus NBRC 9748
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-
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
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Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Thermoascus aurantiacus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 8.4% activity compared to D-glucose Thermoascus aurantiacus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Thermoascus aurantiacus NBRC 6766 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 8.4% activity compared to D-glucose Thermoascus aurantiacus NBRC 6766 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Thermoascus aurantiacus NBRC 9748 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol about 8.4% activity compared to D-glucose Thermoascus aurantiacus NBRC 9748 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol 100% activity Thermoascus aurantiacus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol 100% activity Thermoascus aurantiacus NBRC 6766 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
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?
D-glucose + 2,6-dichlorophenolindophenol 100% activity Thermoascus aurantiacus NBRC 9748 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-raffinose + 2,6-dichlorophenolindophenol about 3.2% activity compared to D-glucose Thermoascus aurantiacus ? + reduced 2,6-dichlorophenolindophenol
-
?
D-raffinose + 2,6-dichlorophenolindophenol about 4.5% activity compared to D-glucose Thermoascus aurantiacus ? + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol about 50% activity compared to D-glucose Thermoascus aurantiacus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol about 40% activity compared to D-glucose Thermoascus aurantiacus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol about 50% activity compared to D-glucose Thermoascus aurantiacus NBRC 6766 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol about 50% activity compared to D-glucose Thermoascus aurantiacus NBRC 9748 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Thermoascus aurantiacus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
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?
maltose + 2,6-dichlorophenolindophenol about 21% activity compared to D-glucose Thermoascus aurantiacus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
additional information no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose Thermoascus aurantiacus ?
-
?
additional information no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose Thermoascus aurantiacus NBRC 6766 ?
-
?
additional information no activity with D-arabinose, D-cellobiose, D-fructose, D-galactose, lactitol, maltitol, D-mannitol, D-mannose, D-sorbitol, sucrose, and D-trehalose Thermoascus aurantiacus NBRC 9748 ?
-
?

Synonyms

Synonyms Comment Organism
FAD-dependent glucose dehydrogenase
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Thermoascus aurantiacus
FADGDH
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Thermoascus aurantiacus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
70
-
-
Thermoascus aurantiacus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40 60 after heat treatment at 40-60┬░C for 15 min, the enzyme maintains its activity close to 100% Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
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Thermoascus aurantiacus
6 7
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Thermoascus aurantiacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 the enzyme is stable for 20 h between pH 3.0 and 11.0 Thermoascus aurantiacus

Cofactor

Cofactor Comment Organism Structure
FAD
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Thermoascus aurantiacus