Any feedback?
Literature summary for 1.1.5.9 extracted from
- Stabilization of fungi-derived recombinant FAD-dependent glucose dehydrogenase by introducing a disulfide bond (2015), Biotechnol. Lett., 37, 1091-1099.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain Origami2(DE3) | Aspergillus flavus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V149C/G190C | the mutant shows a 110 min half-life of thermal inactivation at 45°C, which is 13fold greater than that of the wild type enzyme | Aspergillus flavus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35 | - |
D-glucose | mutant enzyme V149C7G190C, at pH 6.5 and 25°C | Aspergillus flavus |  |
| 35 | - |
D-glucose | wild type enzyme, at pH 6.5 and 25°C | Aspergillus flavus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Aspergillus flavus |
| 61600 | - |
- |
Aspergillus flavus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus flavus | B8MX95 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| resource Q column chromatography | Aspergillus flavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + 2,6-dichlorophenolindophenol | 100% activity | Aspergillus flavus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-xylose + 2,6-dichlorophenolindophenol | 17% activity compared to D-glucose | Aspergillus flavus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| additional information | less than 1% activity with D-mannose, D-maltose, D-galactose, D-allose, D-lactose, D-fructose, sucrose, and cellobiose | Aspergillus flavus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Aspergillus flavus |
| ? | x * 61600, calculated from amino acid sequence | Aspergillus flavus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAD-dependent glucose dehydrogenase | - |
Aspergillus flavus |
| FAD-GDH | - |
Aspergillus flavus |
| FAD-glucose dehydrogenase | - |
Aspergillus flavus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
the wild type enzyme is inactivated rapidly at 45°C, with less than 20% of the initial activity remaining after 25 min and a half-life of inactivation of less than 10 min | Aspergillus flavus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 760 | - |
D-glucose | mutant enzyme V149C7G190C, at pH 6.5 and 25°C | Aspergillus flavus | |
| 780 | - |
D-glucose | wild type enzyme, at pH 6.5 and 25°C | Aspergillus flavus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Aspergillus flavus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22 | - |
D-glucose | mutant enzyme V149C7G190C, at pH 6.5 and 25°C | Aspergillus flavus | |
| 22 | - |
D-glucose | wild type enzyme, at pH 6.5 and 25°C | Aspergillus flavus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
