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Literature summary for 1.1.5.9 extracted from

  • Sakai, G.; Kojima, K.; Mori, K.; Oonishi, Y.; Sode, K.
    Stabilization of fungi-derived recombinant FAD-dependent glucose dehydrogenase by introducing a disulfide bond (2015), Biotechnol. Lett., 37, 1091-1099.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain Origami2(DE3) Aspergillus flavus

Protein Variants

Protein Variants Comment Organism
V149C/G190C the mutant shows a 110 min half-life of thermal inactivation at 45┬░C, which is 13fold greater than that of the wild type enzyme Aspergillus flavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
35
-
D-glucose mutant enzyme V149C7G190C, at pH 6.5 and 25┬░C Aspergillus flavus
35
-
D-glucose wild type enzyme, at pH 6.5 and 25┬░C Aspergillus flavus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Aspergillus flavus
61600
-
-
Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus B8MX95
-
-

Purification (Commentary)

Purification (Comment) Organism
resource Q column chromatography Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol 100% activity Aspergillus flavus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol 17% activity compared to D-glucose Aspergillus flavus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
additional information less than 1% activity with D-mannose, D-maltose, D-galactose, D-allose, D-lactose, D-fructose, sucrose, and cellobiose Aspergillus flavus ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Aspergillus flavus
? x * 61600, calculated from amino acid sequence Aspergillus flavus

Synonyms

Synonyms Comment Organism
FAD-dependent glucose dehydrogenase
-
Aspergillus flavus
FAD-GDH
-
Aspergillus flavus
FAD-glucose dehydrogenase
-
Aspergillus flavus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
45
-
the wild type enzyme is inactivated rapidly at 45┬░C, with less than 20% of the initial activity remaining after 25 min and a half-life of inactivation of less than 10 min Aspergillus flavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
760
-
D-glucose mutant enzyme V149C7G190C, at pH 6.5 and 25┬░C Aspergillus flavus
780
-
D-glucose wild type enzyme, at pH 6.5 and 25┬░C Aspergillus flavus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Aspergillus flavus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22
-
D-glucose mutant enzyme V149C7G190C, at pH 6.5 and 25┬░C Aspergillus flavus
22
-
D-glucose wild type enzyme, at pH 6.5 and 25┬░C Aspergillus flavus