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Literature summary for extracted from

  • Shiota, M.; Yamazaki, T.; Yoshimatsu, K.; Kojima, K.; Tsugawa, W.; Ferri, S.; Sode, K.
    An Fe-S cluster in the conserved Cys-rich region in the catalytic subunit of FAD-dependent dehydrogenase complexes (2016), Bioelectrochemistry, 112, 178-183.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C212S inactive Burkholderia cepacia
C213S the mutant shows 68% of wild type activity Burkholderia cepacia
C218S inactive Burkholderia cepacia
C222S inactive Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
D-glucose wild type enzyme, at pH 7.0, temperature not specified in the publication Burkholderia cepacia
D-glucose mutant enzyme C213S, at pH 7.0, temperature not specified in the publication Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
Burkholderia cepacia


Organism UniProt Comment Textmining
Burkholderia cepacia

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel affinity chromatography Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol
Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol


Subunits Comment Organism
? x * 59832, catalytic subunit, calculated from amino acid sequence Burkholderia cepacia


Synonyms Comment Organism
FAD dependent glucose dehydrogenase
Burkholderia cepacia
Burkholderia cepacia


Cofactor Comment Organism Structure
Burkholderia cepacia
Burkholderia cepacia