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Literature summary for 1.1.5.9 extracted from

  • Ozawa, K.; Iwasa, H.; Sasaki, N.; Kinoshita, N.; Hiratsuka, A.; Yokoyama, K.
    Identification and characterization of thermostable glucose dehydrogenases from thermophilic filamentous fungi (2017), Appl. Microbiol. Biotechnol., 101, 173-183.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and Pichia pastoris Rasamsonia emersonii
expressed in Escherichia coli and Pichia pastoris Thermoascus crustaceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
x * 58000, SDS-PAGE Thermoascus crustaceus
60000
-
x * 60000, SDS-PAGE Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii A0A1E1GL61
-
-
Rasamsonia emersonii NBRC 31232 A0A1E1GL61
-
-
Thermoascus crustaceus A0A1E1GL87
-
-
Thermoascus crustaceus NBRC 9129 A0A1E1GL87
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Rasamsonia emersonii
glycoprotein
-
Thermoascus crustaceus

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Rasamsonia emersonii
Ni-NTA agarose column chromatography Thermoascus crustaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 11.5% activity compared to D-glucose Rasamsonia emersonii 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 60.1% activity compared to D-glucose Thermoascus crustaceus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 60.1% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 11.5% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Rasamsonia emersonii D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Thermoascus crustaceus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Thermoascus crustaceus NBRC 9129 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Rasamsonia emersonii NBRC 31232 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichloroindophenol 24.8% activity compared to D-glucose Rasamsonia emersonii D-xylono-1,5-lactone + 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichloroindophenol 24.8% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 D-xylono-1,5-lactone + 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol 27.6% activity compared to D-glucose Thermoascus crustaceus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + 2,6-dichlorophenolindophenol 27.6% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltotriose + 2,6-dichlorophenolindophenol 3.4% activity compared to D-glucose Rasamsonia emersonii maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltotriose + 2,6-dichlorophenolindophenol 6.8% activity compared to D-glucose Thermoascus crustaceus maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltotriose + 2,6-dichlorophenolindophenol 6.8% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltotriose + 2,6-dichlorophenolindophenol 3.4% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Rasamsonia emersonii ?
-
?
additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Thermoascus crustaceus ?
-
?
additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Thermoascus crustaceus NBRC 9129 ?
-
?
additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Rasamsonia emersonii NBRC 31232 ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Rasamsonia emersonii
? x * 58000, SDS-PAGE Thermoascus crustaceus

Synonyms

Synonyms Comment Organism
FAD-dependent glucose dehydrogenase
-
Rasamsonia emersonii
FAD-dependent glucose dehydrogenase
-
Thermoascus crustaceus
FAD-GDH
-
Rasamsonia emersonii
FAD-GDH
-
Thermoascus crustaceus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
60 65
-
Thermoascus crustaceus
65 70
-
Rasamsonia emersonii

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40 70 after heat treatment at 40-70┬░C for 15 min, unglycosylated enzyme maintains nearly 100% activity at temperatures up to 45┬░C, but completely loses activity at 60┬░C, while glycosylated enzyme displays high thermostability, maintaining 89% activity at 60┬░C. The melting temperature of the glycosylated enzyme is 66.4┬░C Rasamsonia emersonii
40 62 unglycosylated enzym e is stable at temperatures up to 45┬░C , while glycosylatedenzyme maintains 75% activity at 60┬░C. The melting temperature of the glycosylated enzyme is 62.5┬░C Thermoascus crustaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Rasamsonia emersonii
5
-
-
Thermoascus crustaceus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 9 the enzyme remains stable between pH 2.0 and 9.0 Rasamsonia emersonii
2 9 the enzyme remains stable between pH 2.0 and 9.0 Thermoascus crustaceus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rasamsonia emersonii
FAD
-
Thermoascus crustaceus