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Literature summary for 1.1.5.9 extracted from

  • Fapyane, D.; Lee, S.J.; Kang, S.H.; Lim, D.H.; Cho, K.K.; Nam, T.H.; Ahn, J.P.; Ahn, J.H.; Kim, S.W.; Chang, I.S.
    High performance enzyme fuel cells using a genetically expressed FAD-dependent glucose dehydrogenase alpha-subunit of Burkholderia cepacia immobilized in a carbon nanotube electrode for low glucose conditions (2013), Phys. Chem. Chem. Phys., 15, 9508-9512.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21RIL, no GDH activity when cells expressing the alpha-subunit alone Burkholderia cepacia

Protein Variants

Protein Variants Comment Organism
additional information the purified recombinnat enzyme is used as an anode catalyst for enzyme fuel cells, method, overview. The recombinant FAD-GDH is able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. Heterogeneous electron transfer coefficient of FAD-GDHĀ–menadione on a glassy carbon electrode was 10.73/s Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4638
-
D-glucose pH and temperature not specified in the publication Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
1 * 59800 + 1 * 18000, SDS-PAGE Burkholderia cepacia
59800
-
1 * 59800 + 1 * 18000, SDS-PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + a quinone Burkholderia cepacia
-
D-glucono-1,5-lactone + a quinol
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21RIL Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + a quinone
-
Burkholderia cepacia D-glucono-1,5-lactone + a quinol
-
?
D-glucose + menadione
-
Burkholderia cepacia D-glucono-1,5-lactone + menadiol
-
?
D-glucose + oxidized 2,6-dichlorophenol indophenol
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?

Subunits

Subunits Comment Organism
dimer 1 * 59800 + 1 * 18000, SDS-PAGE Burkholderia cepacia

Synonyms

Synonyms Comment Organism
FAD-dependent glucose dehydrogenase
-
Burkholderia cepacia
FAD-GDH
-
Burkholderia cepacia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
69.54
-
D-glucose pH and temperature not specified in the publication Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
FAD dependent on, bound in the catalytic site Burkholderia cepacia