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Literature summary for 1.1.5.9 extracted from

  • Monosik, R.; Stredansky, M.; Luspai, K.; Magdolen, P.; Sturdik, E.
    Amperometric glucose biosensor utilizing FAD-dependent glucose dehydrogenase immobilized on nanocomposite electrode (2012), Enzyme Microb. Technol., 50, 227-232.
    View publication on PubMed

Application

Application Comment Organism
analysis the enzyme can be used as O2-independent biosensor for glucoe detection Aspergillus oryzae
diagnostics the enzyme can be used as O2-independent biosensor for glucoe detection Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
additional information amperometric glucose biosensor utilizing FAD-dependent glucose dehydrogenase immobilized on nanocomposite electrode. Unlike the common glucose oxidase based biosensor, the presented biosensors is O2-independent, method and biosensorevlauation, overview. Polyphenols also do not interfere at used measuring conditions. Determination of D-glucose in beverages and wines using biosensors, HPLC and enzymatic-spectrophotometric assay, overview Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.09
-
D-glucose pH 7.4, 37┬░C, enzyme immobilized on amperometric electrode Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + a quinone Aspergillus oryzae
-
D-glucono-1,5-lactone + a reduced quinol
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + a quinone
-
Aspergillus oryzae D-glucono-1,5-lactone + a reduced quinol
-
?

Synonyms

Synonyms Comment Organism
FAD-dependent glucose dehydrogenase
-
Aspergillus oryzae
GDH-FAD
-
Aspergillus oryzae

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5
-
Aspergillus oryzae

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Aspergillus oryzae

General Information

General Information Comment Organism
additional information amperometric glucose biosensor utilizing FAD-dependent glucose dehydrogenase immobilized on nanocomposite electrode. Unlike the common glucose oxidase based biosensor, the presented biosensors is O2-independent, method and biosensorevlauation, overview. Polyphenols also do not interfere at used measuring conditions. Determination of D-glucose in beverages and wines using biosensors, HPLC and enzymatic-spectrophotometric assay, overview Aspergillus oryzae