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Literature summary for 1.1.5.9 extracted from

  • Zafar, M.N.; Beden, N.; Leech, D.; Sygmund, C.; Ludwig, R.; Gorton, L.
    Characterization of different FAD-dependent glucose dehydrogenases for possible use in glucose-based biosensors and biofuel cells (2012), Anal. Bioanal. Chem., 402, 2069-2077.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis possible use of the enzyme as amperometric glucose biosensor Komagataella pastoris
analysis possible use of the enzyme as amperometric glucose biosensor Colletotrichum gloeosporioides
analysis possible use of the enzyme as amperometric glucose biosensor Aspergillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Pichia pastoris Colletotrichum gloeosporioides

Protein Variants

Protein Variants Comment Organism
additional information the FAD-dependent glucose dehydrogenase is evaluated electrochemically connected to an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)2 (PVI)10Cl]+ on graphite electrode for possible use in glucose-based biosensors and biofuel cells, method and sensitivity, overview Komagataella pastoris
additional information the FAD-dependent glucose dehydrogenase is evaluated electrochemically connected to an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)2 (PVI)10Cl]+ on graphite electrode for possible use in glucose-based biosensors and biofuel cells, method and sensitivity, overview Colletotrichum gloeosporioides
additional information the FAD-dependent glucose dehydrogenase is evaluated electrochemically connected to an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)2 (PVI)10Cl]+ on graphite electrode for possible use in glucose-based biosensors and biofuel cells, method and sensitivity, overview Aspergillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
x * 67000, deglycosylated recombinant enzyme, SDS-PAGE Aspergillus sp.
88000 131000
-
Komagataella pastoris
95000 135000 recombinant enzyme Colletotrichum gloeosporioides
97000
-
deglycosylated enzyme, gel filtration Aspergillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + a quinone Komagataella pastoris
-
D-glucono-1,5-lactone + a quinol
-
?
D-glucose + a quinone Colletotrichum gloeosporioides
-
D-glucono-1,5-lactone + a quinol
-
?
D-glucose + a quinone Aspergillus sp.
-
D-glucono-1,5-lactone + a quinol
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-
Colletotrichum gloeosporioides
-
-
-
Komagataella pastoris
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Komagataella pastoris
glycoprotein
-
Colletotrichum gloeosporioides
glycoprotein
-
Aspergillus sp.

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Aspergillus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
795
-
pH and temperature not specified in the publication Aspergillus sp.
836
-
pH and temperature not specified in the publication Komagataella pastoris
878
-
pH and temperature not specified in the publication Colletotrichum gloeosporioides

Storage Stability

Storage Stability Organism
GcGDH/Os-polymer modified electrode under storage conditions of 50 mM phosphate buffer, pH 7.4, 4 Ā°C, injection of 0.05 ml of a 5 mM glucose solution into the electrochemical cell for 6 days, the biosensor keeps more than 90% of its initial activity after the first day Colletotrichum gloeosporioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + a quinone
-
Colletotrichum gloeosporioides 2-deoxy-D-glucono-1,5-lactone + a quinol
-
?
cellobiose + a quinone low activity Colletotrichum gloeosporioides ? + a quinol
-
?
D-glucose + a quinone
-
Komagataella pastoris D-glucono-1,5-lactone + a quinol
-
?
D-glucose + a quinone
-
Colletotrichum gloeosporioides D-glucono-1,5-lactone + a quinol
-
?
D-glucose + a quinone
-
Aspergillus sp. D-glucono-1,5-lactone + a quinol
-
?
D-xylose + ferricenium ion
-
Colletotrichum gloeosporioides D-xylono-1,5-lactone + ferrocenium ion
-
?
glutamine + a quinone low activity Colletotrichum gloeosporioides ? + a quinol
-
?
additional information substrate specificity, overview. Lactose, gluconic acid, mannose, mannitol, sorbitol, galactose, sucrose, maltose, arabinose, xylitol, rhamnose, fucose, trehalose, and fructose are poor or no substrates Colletotrichum gloeosporioides ?
-
?

Subunits

Subunits Comment Organism
? x * 67000, deglycosylated recombinant enzyme, SDS-PAGE Aspergillus sp.

Synonyms

Synonyms Comment Organism
FAD-dependent glucose dehydrogenase
-
Komagataella pastoris
FAD-dependent glucose dehydrogenase
-
Colletotrichum gloeosporioides
FAD-dependent glucose dehydrogenase
-
Aspergillus sp.
FADGDH
-
Komagataella pastoris
FADGDH
-
Colletotrichum gloeosporioides
FADGDH
-
Aspergillus sp.
flavin adenine dinucleotide-dependent glucose dehydrogenase
-
Komagataella pastoris
flavin adenine dinucleotide-dependent glucose dehydrogenase
-
Colletotrichum gloeosporioides
flavin adenine dinucleotide-dependent glucose dehydrogenase
-
Aspergillus sp.

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Komagataella pastoris
FAD dependent on Colletotrichum gloeosporioides
FAD dependent on Aspergillus sp.