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Literature summary for 1.1.5.9 extracted from

  • Yamazaki, T.; Tsugawa, W.; Sode, K.
    Subunit Analyses of a Novel Thermostable Glucose Dehydrogenase Showing Different Temperature Properties According to Its Quaternary Structure (1999), Appl. Biochem. Biotechnol., 77-79, 325-335.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Urea urea causes a dose-dependent but reversible inhibition by dissoziation of the hetero-oligomeric enzyme comparable with the effect of heat-treatment, 3.5 M urea reduces GDH activity by 20%, 5 M urea by 80% and 8 M urea by ca. 90% Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
D-glucose at 40┬░C after preincubation of the enzyme at 70┬░C for 30 min Burkholderia cepacia
1.4
-
phenazine methosulfate at 40┬░C Burkholderia cepacia
2.5
-
D-glucose at 40┬░C Burkholderia cepacia
2.7
-
phenazine methosulfate at 40┬░C after preincubation of the enzyme at 70┬░C for 30 min Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses Burkholderia cepacia
67000
-
SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
bacterial strain SM4 is used, isolated from a soil sample near a Japanese hot spring
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
290 micromol/min/mg Vmax of oxidation of glucose at 40┬░C Burkholderia cepacia
additional information
-
4.3 micromol/min/mg Vmax of oxidation of phenazine methosulfate at 40┬░C after preincubation of the enzyme at 70┬░C for 30 min Burkholderia cepacia
additional information
-
470 micromol/min/mg Vmax of oxidation of phenazine methosulfate at 40┬░C Burkholderia cepacia
additional information
-
66 micromol/min/mg Vmax of oxidation of glucose at 40┬░C after preincubation of the enzyme at 70┬░C for 30 min Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + phenazine methosulfate
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?

Subunits

Subunits Comment Organism
heteromer SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses Burkholderia cepacia

Synonyms

Synonyms Comment Organism
GDH
-
Burkholderia cepacia
glucose dehydrogenase
-
Burkholderia cepacia

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
70
-
after preincubation with 5.8 M urea for 30 min at 25┬░C Burkholderia cepacia

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
50 80 after preincubation with 5.8 M urea for 30 min at 25┬░C Burkholderia cepacia