Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.5.9 extracted from

  • Bak, T.G.
    Studies on glucose dehydrogenase of Aspergillus oryzae. II. Purification and physical and chemical properties (1967), Biochim. Biophys. Acta, 139, 277-293.
    View publication on PubMed

General Stability

General Stability Organism
glucose, 0.2 M, protects during heat treatment Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
low speed sedimentation without reaching equilibrium Aspergillus oryzae
120000
-
gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 24% carbohydrate consisting of glucose, mannose and hexosamines Aspergillus oryzae

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Aspergillus oryzae
-
mycelium
-
Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
-
Aspergillus oryzae

Storage Stability

Storage Stability Organism
-18┬░C, 0.05 M potassium phosphate buffer, pH 6.5, 10% loss of activity after 3 months, 50% loss of activity after 6 months Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol
-
Aspergillus oryzae D-gluconic acid + reduced 2,6-dichlorophenolindophenol
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30
-
15 min, stable Aspergillus oryzae
50
-
15 min, about 40% loss of activity Aspergillus oryzae
60
-
15 min, complete loss of activity Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
30┬░C, 2 h, about 40% loss of activity Aspergillus oryzae
6 8 30┬░C, 2 h, stable, rapid inactivation outside this range Aspergillus oryzae

Cofactor

Cofactor Comment Organism Structure
FAD contains 1 mol per mol of enzyme Aspergillus oryzae