BRENDA - Enzyme Database
show all sequences of 1.1.5.7

Purification and characterization of membrane-bound quinoprotein cyclic alcohol dehydrogenase from Gluconobacter frateurii CHM 9

Moonmangmee, D.; Fujii, Y.; Toyama, H.; Theeragool, G.; Lotong, N.; Matsushita, K.; Adachi, O.; Biosci. Biotechnol. Biochem. 65, 2763-2772 (2001)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
Cyclopentanol
pH 5.5, 25°C
Gluconobacter frateurii
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm
Gluconobacter frateurii
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
addition of pyrroloquinoline quinone and Ca2+ converts the apo-enzyme to the holoenzyme
Gluconobacter frateurii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
83000
-
x * 83000, SDS-PAGE
Gluconobacter frateurii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Gluconobacter frateurii
the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible
?
-
-
?
additional information
Gluconobacter frateurii CHM 9
the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Gluconobacter frateurii
-
CHM 9
-
Gluconobacter frateurii CHM 9
-
CHM 9
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Gluconobacter frateurii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
44.3
-
-
Gluconobacter frateurii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone
74% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
(1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone
74% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii CHM 9
?
-
-
-
ir
(1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone
11% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
(1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone
11% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii CHM 9
?
-
-
-
ir
(2R,3R)-2,3-butanediol + pyrroloquinoline quinone
41% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
1,2-butanediol + pyrroloquinoline quinone
63% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
1,3-butanediol + pyrroloquinoline quinone
12% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
1,3-cyclopentanediol + pyrroloquinoline quinone
73% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
1,4-cyclohexanediol + pyrroloquinoline quinone
14% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
2,3-butanediol + pyrroloquinoline quinone
186% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
2,4-pentanediol + pyrroloquinoline quinone
16% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
2-butanol + pyrroloquinoline quinone
41% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
butane-2-one + reduced pyrroloquinoline quinone
-
-
-
ir
2-butanol + pyrroloquinoline quinone
41% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii CHM 9
butane-2-one + reduced pyrroloquinoline quinone
-
-
-
ir
2-hexanol + pyrroloquinoline quinone
10% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
2-hexanone + pyrroloquinoline quinol
-
-
-
ir
2-methyl-2,4-pentanediol + pyrroloquinoline quinone
17% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
2-propanol + pyrroloquinoline quinone
17% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
acetone + pyrroloquinoline quinol
-
-
-
ir
3-pentanol + pyrroloquinoline quinone
74% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
3-pentanone + pyrroloquinoline quinol
-
-
-
ir
cis-1,2-cyclohexanediol + pyrroloquinoline quinone
88% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
cis-1,2-cyclopentanediol + pyrroloquinoline quinone
181% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
cis-4-cyclopentene-1,3-diol + pyrroloquinoline quinone
32% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
cyclobutanol + pyrroloquinoline quinone
73% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
cyclobutanone + pyrroloquinoline quinol
-
-
-
ir
cyclohexanol + pyrroloquinoline quinone
73% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
cyclohexanone + pyrroloquinoline quinol
-
-
-
ir
cyclooctanol + pyrroloquinoline quinone
137% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
cyclooctanone + pyrroloquinoline quinol
-
-
-
ir
cyclopentanol + pyrroloquinoline quinone
only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+
696803
Gluconobacter frateurii
cyclopentanone + pyrroloquinoline quinol
-
-
-
ir
D-arabitol + pyrroloquinoline quinone
78% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
D-mannitol + pyrroloquinoline quinone
25% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
D-sorbitol + pyrroloquinoline quinone
34% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
glycerol + pyrroloquinoline quinone
59% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
meso-erythritol + pyrroloquinoline quinone
100% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
additional information
the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible
696803
Gluconobacter frateurii
?
-
-
-
?
additional information
the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols
696803
Gluconobacter frateurii
?
-
-
-
?
additional information
the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible
696803
Gluconobacter frateurii CHM 9
?
-
-
-
?
additional information
the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols
696803
Gluconobacter frateurii CHM 9
?
-
-
-
?
ribitol + pyrroloquinoline quinone
34% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
Subunits
Subunits
Commentary
Organism
?
x * 83000, SDS-PAGE
Gluconobacter frateurii
Synonyms
Synonyms
Commentary
Organism
MCAD
-
Gluconobacter frateurii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter frateurii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
assay at
Gluconobacter frateurii
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+
Gluconobacter frateurii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+
Gluconobacter frateurii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
Cyclopentanol
pH 5.5, 25°C
Gluconobacter frateurii
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm
Gluconobacter frateurii
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
addition of pyrroloquinoline quinone and Ca2+ converts the apo-enzyme to the holoenzyme
Gluconobacter frateurii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
83000
-
x * 83000, SDS-PAGE
Gluconobacter frateurii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Gluconobacter frateurii
the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible
?
-
-
?
additional information
Gluconobacter frateurii CHM 9
the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gluconobacter frateurii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
44.3
-
-
Gluconobacter frateurii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone
74% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
(1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone
74% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii CHM 9
?
-
-
-
ir
(1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone
11% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
(1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone
11% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii CHM 9
?
-
-
-
ir
(2R,3R)-2,3-butanediol + pyrroloquinoline quinone
41% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
1,2-butanediol + pyrroloquinoline quinone
63% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
1,3-butanediol + pyrroloquinoline quinone
12% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
1,3-cyclopentanediol + pyrroloquinoline quinone
73% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
1,4-cyclohexanediol + pyrroloquinoline quinone
14% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
2,3-butanediol + pyrroloquinoline quinone
186% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
2,4-pentanediol + pyrroloquinoline quinone
16% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
2-butanol + pyrroloquinoline quinone
41% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
butane-2-one + reduced pyrroloquinoline quinone
-
-
-
ir
2-butanol + pyrroloquinoline quinone
41% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii CHM 9
butane-2-one + reduced pyrroloquinoline quinone
-
-
-
ir
2-hexanol + pyrroloquinoline quinone
10% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
2-hexanone + pyrroloquinoline quinol
-
-
-
ir
2-methyl-2,4-pentanediol + pyrroloquinoline quinone
17% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
2-propanol + pyrroloquinoline quinone
17% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
acetone + pyrroloquinoline quinol
-
-
-
ir
3-pentanol + pyrroloquinoline quinone
74% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
3-pentanone + pyrroloquinoline quinol
-
-
-
ir
cis-1,2-cyclohexanediol + pyrroloquinoline quinone
88% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
cis-1,2-cyclopentanediol + pyrroloquinoline quinone
181% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
cis-4-cyclopentene-1,3-diol + pyrroloquinoline quinone
32% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
cyclobutanol + pyrroloquinoline quinone
73% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
cyclobutanone + pyrroloquinoline quinol
-
-
-
ir
cyclohexanol + pyrroloquinoline quinone
73% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
cyclohexanone + pyrroloquinoline quinol
-
-
-
ir
cyclooctanol + pyrroloquinoline quinone
137% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
cyclooctanone + pyrroloquinoline quinol
-
-
-
ir
cyclopentanol + pyrroloquinoline quinone
only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+
696803
Gluconobacter frateurii
cyclopentanone + pyrroloquinoline quinol
-
-
-
ir
D-arabitol + pyrroloquinoline quinone
78% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
D-mannitol + pyrroloquinoline quinone
25% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
D-sorbitol + pyrroloquinoline quinone
34% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
glycerol + pyrroloquinoline quinone
59% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
meso-erythritol + pyrroloquinoline quinone
100% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
additional information
the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible
696803
Gluconobacter frateurii
?
-
-
-
?
additional information
the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols
696803
Gluconobacter frateurii
?
-
-
-
?
additional information
the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible
696803
Gluconobacter frateurii CHM 9
?
-
-
-
?
additional information
the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols
696803
Gluconobacter frateurii CHM 9
?
-
-
-
?
ribitol + pyrroloquinoline quinone
34% of the activity compared to cyclopentanol
696803
Gluconobacter frateurii
?
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 83000, SDS-PAGE
Gluconobacter frateurii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter frateurii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
assay at
Gluconobacter frateurii
Other publictions for EC 1.1.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696803
Moonmangmee
Purification and characterizat ...
Gluconobacter frateurii, Gluconobacter frateurii CHM 9
Biosci. Biotechnol. Biochem.
65
2763-2772
2001
-
-
-
-
-
-
-
1
1
1
1
2
-
2
-
-
1
-
-
-
1
-
34
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
1
2
-
-
-
1
-
-
1
-
34
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-