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Literature summary for 1.1.5.4 extracted from

  • Mogi, T.; Murase, Y.; Mori, M.; Shiomi, K.; Omura, S.; Paranagama, M.P.; Kita, K.
    Polymyxin B identified as an inhibitor of alternative NADH dehydrogenase and malate: quinone oxidoreductase from the Gram-positive bacterium Mycobacterium smegmatis (2009), J. Biochem., 146, 491-499.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(S)-malate in the presence of polymyxin B, enzyme kinetics changes from the Michaelis-Menten type to substrate inhibition kinetics with the substrate inhibition constant Ksi of 57.4 microg/ml Mycolicibacterium smegmatis
nanaomycin A naphthoquinone derivative Mycolicibacterium smegmatis
Polymyxin B cationic decapeptide. Primary site of action is the quinone-binding site, amino acid sequence is examined and possible binding sites for L-malate and quinones are found Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
ubiquinone-1 pH 7.4, 25°C Mycolicibacterium smegmatis
2.6
-
(S)-malate pH 7.4, 25°C Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + ubiquinone-1
-
Mycolicibacterium smegmatis oxaloacetate + ubiquinol-1
-
?

Synonyms

Synonyms Comment Organism
malate:quinone oxidoreductase
-
Mycolicibacterium smegmatis
malate:quinone reductase
-
Mycolicibacterium smegmatis
MQR
-
Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mycolicibacterium smegmatis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information in the presence of polymyxin B, enzyme kinetics changes from the Michaelis-Menten type to substrate inhibition kinetics with the substrate inhibition constant Ksi of 57.4 microg/ml. Polymyxin B inhibits the malate-dependent reaction noncompetitively with the Ki value of 7.0 microg/ml Mycolicibacterium smegmatis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
IC50 for polymyxin B is 4.2 microg/ml, IC50 for nanaomycin is 49 microg/ml Mycolicibacterium smegmatis additional information