Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-malate | in the presence of polymyxin B, enzyme kinetics changes from the Michaelis-Menten type to substrate inhibition kinetics with the substrate inhibition constant Ksi of 57.4 microg/ml | Mycolicibacterium smegmatis | |
nanaomycin A | naphthoquinone derivative | Mycolicibacterium smegmatis | |
Polymyxin B | cationic decapeptide. Primary site of action is the quinone-binding site, amino acid sequence is examined and possible binding sites for L-malate and quinones are found | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
ubiquinone-1 | pH 7.4, 25°C | Mycolicibacterium smegmatis | |
2.6 | - |
(S)-malate | pH 7.4, 25°C | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + ubiquinone-1 | - |
Mycolicibacterium smegmatis | oxaloacetate + ubiquinol-1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malate:quinone oxidoreductase | - |
Mycolicibacterium smegmatis |
malate:quinone reductase | - |
Mycolicibacterium smegmatis |
MQR | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mycolicibacterium smegmatis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | in the presence of polymyxin B, enzyme kinetics changes from the Michaelis-Menten type to substrate inhibition kinetics with the substrate inhibition constant Ksi of 57.4 microg/ml. Polymyxin B inhibits the malate-dependent reaction noncompetitively with the Ki value of 7.0 microg/ml | Mycolicibacterium smegmatis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC50 for polymyxin B is 4.2 microg/ml, IC50 for nanaomycin is 49 microg/ml | Mycolicibacterium smegmatis | additional information |