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Literature summary for 1.1.5.4 extracted from

  • Foerster-Fromme, K.; Jendrossek, D.
    Malate:quinone oxidoreductase (MqoB) is required for growth on acetate and linear terpenes in Pseudomonas citronellolis (2005), FEMS Microbiol. Lett., 246, 25-31.
    View publication on PubMed
Search for more literature for 1.1.5.4

Cloned(Commentary)

Cloned (Comment) Organism
gene mqoB, DNA and amino acid sequence determination, analysis, and comparison, expression of mutant enzymes in Escherichia coli strain JM109 Pseudomonas citronellolis
gene mqoB, DNA and amino acid sequence determination, analysis, and comparison, expression of mutant enzymes in Escherichia coli strain JM109 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of mqoB mutants by Tn5-Tc transposon insertion, class I mutants show strongly reduced growth on citronellol and citronellic acid, class II mutants grow normally on citronellic acid but reduced on citronellol, class III mutants are auxotroph, overview Pseudomonas citronellolis
additional information construction of mqoB mutants by Tn5-Tc transposon insertion, class I mutants show strongly reduced growth on citronellol and citronellic acid, class II mutants grow normally on citronellic acid but reduced on citronellol, class III mutants are auxotroph, overview Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas aeruginosa 16020
-
membrane mainly Pseudomonas citronellolis 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas citronellolis the enzyme is required for growth on acetate and linear terpenes such as citronellol and citronellic acid ?
-
 ?
additional information Pseudomonas aeruginosa the enzyme is required for growth on acetate and linear terpenes such as citronellol and citronellic acid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HVF1 gene mqoB; gene mqoB
-
Pseudomonas citronellolis Q5ECC3 gene mqoB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + 2,6-dichlorphenolindophenol assay in presence of 2,3-dimethoxy-5-methyl-1,4-benzoquinone Pseudomonas citronellolis oxaloacetate + reduced 2,6-dichlorphenolindophenol
-
 ?
(S)-malate + 2,6-dichlorphenolindophenol assay in presence of 2,3-dimethoxy-5-methyl-1,4-benzoquinone Pseudomonas aeruginosa oxaloacetate + reduced 2,6-dichlorphenolindophenol
-
 ?
additional information the enzyme is required for growth on acetate and linear terpenes such as citronellol and citronellic acid Pseudomonas citronellolis ?
-
 ?
additional information the enzyme is required for growth on acetate and linear terpenes such as citronellol and citronellic acid Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
malate:quinone oxidoreductase
-
 Pseudomonas citronellolis
malate:quinone oxidoreductase
-
 Pseudomonas aeruginosa
Mqo
-
 Pseudomonas citronellolis
Mqo
-
 Pseudomonas aeruginosa
MqoB
-
 Pseudomonas citronellolis
MqoB
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas citronellolis
30
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas citronellolis
7.5
-
 assay at Pseudomonas aeruginosa