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Literature summary for 1.1.5.2 extracted from

  • Xue, Q.; Wei, Z.; Sun, W.; Cui, F.; Yu, S.; Zhou, Q.; Liu, J.
    2-Keto-D-gluconate-yielding membrane-bound D-glucose dehydrogenase from Arthrobacter globiformis C224: purification and characterization (2015), Molecules, 20, 846-862.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
n-hexane 92.1% increase of activity at 80% (v/v) Arthrobacter globiformis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slight inhibitory effect Arthrobacter globiformis
Cu2+ complete inhibition at 10 mM Arthrobacter globiformis
D-gluconic acid non-competitive inhibitor to D-glucose and competitive inhibitor to N-methylphenazonium methyl sulfate Arthrobacter globiformis
D-glucose
-
Arthrobacter globiformis
EDTA complete inhibition at 0.5 mM Arthrobacter globiformis
ethanol 3.6% residual activity at 80% (v/v) Arthrobacter globiformis
Fe3+ complete inhibition at 10 mM Arthrobacter globiformis
methanol
-
Arthrobacter globiformis
Mn2+ slight inhibitory effect Arthrobacter globiformis
N-methylphenazonium methyl sulfate
-
Arthrobacter globiformis
Zn2+ slight inhibitory effect Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
D-glucose at pH 5.0 and 45°C Arthrobacter globiformis
0.34
-
D-xylose at pH 5.0 and 45°C Arthrobacter globiformis
0.4
-
N-methylphenazonium methyl sulfate with D-glucose as cosubstrate, at pH 5.0 and 45°C Arthrobacter globiformis
0.46
-
D-galactose at pH 5.0 and 45°C Arthrobacter globiformis
0.59
-
maltose at pH 5.0 and 45°C Arthrobacter globiformis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Arthrobacter globiformis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20% increase of activity at 1 mM Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
x * 87000, SDS-PAGE Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ubiquinone Arthrobacter globiformis preferred substrate D-glucono-1,5-lactone + ubiquinol
-
?
D-glucose + ubiquinone Arthrobacter globiformis C224 preferred substrate D-glucono-1,5-lactone + ubiquinol
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Arthrobacter globiformis C224
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Triton X-114 phase precipitation, acetone precipitation, polyethylene glcyol 6000 precipitation, ethanol precipitation, and hydroxylapatite column chromatography Arthrobacter globiformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
crude extract, at pH 5.0 and 45°C Arthrobacter globiformis
88.1
-
after 183.5fold purification, at pH 5.0 and 45°C Arthrobacter globiformis

Storage Stability

Storage Stability Organism
4°C, at pH 4.0 or 9.0, 2 days, complete loss of activity Arthrobacter globiformis
4°C, at pH 6.0-7.0, 2 days, no loss of activity Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose + N-methylphenazonium methyl sulfate 8.0% activity compared to D-glucose Arthrobacter globiformis D-arabinono-1,5-lactone + ?
-
?
D-arabinose + N-methylphenazonium methyl sulfate 8.0% activity compared to D-glucose Arthrobacter globiformis C224 D-arabinono-1,5-lactone + ?
-
?
D-galactose + N-methylphenazonium methyl sulfate 20% activity compared to D-glucose Arthrobacter globiformis D-galactono-1,5-lactone + ?
-
?
D-galactose + N-methylphenazonium methyl sulfate 20% activity compared to D-glucose Arthrobacter globiformis C224 D-galactono-1,5-lactone + ?
-
?
D-glucose + ferricyanide D-glucose is the preferred substrate Arthrobacter globiformis D-glucono-1,5-lactone + ferrocyanide
-
?
D-glucose + ferricyanide D-glucose is the preferred substrate Arthrobacter globiformis C224 D-glucono-1,5-lactone + ferrocyanide
-
?
D-glucose + N-methylphenazonium methyl sulfate D-glucose is the preferred substrate Arthrobacter globiformis D-glucono-1,5-lactone + ?
-
?
D-glucose + ubiquinone preferred substrate Arthrobacter globiformis D-glucono-1,5-lactone + ubiquinol
-
?
D-glucose + ubiquinone preferred substrate Arthrobacter globiformis C224 D-glucono-1,5-lactone + ubiquinol
-
?
D-xylose + N-methylphenazonium methyl sulfate 22% activity compared to D-glucose Arthrobacter globiformis D-xylono-1,5-lactone + ?
-
?
maltose + N-methylphenazonium methyl sulfate 17% activity compared to D-glucose Arthrobacter globiformis maltono-1,5-lactone + ?
-
?
additional information no activity with D-gluconate, D-sorbose, D-mannose, D-fructose, malic acid, sucrose, and citric acid Arthrobacter globiformis ?
-
?
additional information no activity with D-gluconate, D-sorbose, D-mannose, D-fructose, malic acid, sucrose, and citric acid Arthrobacter globiformis C224 ?
-
?

Subunits

Subunits Comment Organism
? x * 87000, SDS-PAGE Arthrobacter globiformis
? x * 87067, calculated from amino acid sequence Arthrobacter globiformis

Synonyms

Synonyms Comment Organism
GlcDH
-
Arthrobacter globiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Arthrobacter globiformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 85 the enzyme reaches to the maximum activity with the increase of reaction temperature from 15 to 45 °C, and follows the sharp decline of activity to 30% at 55°C, and shows no activity at 85°C Arthrobacter globiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 low temperature of below 40°C has the stable and highest enzyme activity after kept for 120 min. Increase of storage temperature to over 50°C inhibits the activity of above 58% after 10 min treatment, while no activity is detected after incubation for 60 min at 60 or 70°C Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Arthrobacter globiformis

pH Range

pH Minimum pH Maximum Comment Organism
4 6 the enzyme relative activity decreases from optimum at pH 5.0 to approximately 51% or 36% when the pH drops to 4.0 or increases to 6.0, respectively Arthrobacter globiformis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
11.4
-
N-methylphenazonium methyl sulfate at pH 5.0 and 45°C Arthrobacter globiformis
14.8
-
D-glucose at pH 5.0 and 45°C Arthrobacter globiformis