Cloned (Comment) | Organism |
---|---|
gene gox0265 or mgdh, overexpression of Strep-tagged membrane-bound glucose dehydrogenase from vector pBBR1p452 in membranes of an enzyme-deficient Gluconobacter oxydans strain, that shows 9fold higher enzyme expression level and 5fold increased mGDH activity, and 3fold increased oxygen consumption rates, compared to the wild-type strain, gluconate production and analysis of whole cells, overview | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
D-glucose | recombinant Strep-tagged enzyme, pH 6.0, 25°C | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme is a membrane integral quinoprotein | Gluconobacter oxydans | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
x * 89000, recombinant Strep-tagged enzyme, SDS-PAGE | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | Gluconobacter oxydans | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
gene gox0265 or mgdh | - |
Purification (Comment) | Organism |
---|---|
recombinant Strep-tagged membrane-bound glucose dehydrogenase from from membranes of the expressing Gluconobacter oxydans strain by streptactin affinity chromatography to homogeneity | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
gluconate production and analysis of whole cells | Gluconobacter oxydans |
150 | - |
purified recombinant Strep-tagged enzyme, pH 6.0, 25°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + ubiquinone | - |
Gluconobacter oxydans | 2-deoxy-D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-allose + ubiquinone | - |
Gluconobacter oxydans | ? + ubiquinol | - |
? | |
D-fucose + ubiquinone | - |
Gluconobacter oxydans | ? + ubiquinol | - |
? | |
D-glucose + ubiquinone | - |
Gluconobacter oxydans | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | preferred substrate, assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Gluconobacter oxydans | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-xylose + ubiquinone | - |
Gluconobacter oxydans | D-xylono-1,5-lactone + ubiquinol | - |
? | |
additional information | the enzyme catalyzes only oxidation of D-aldoses and aldose derivatives | Gluconobacter oxydans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 89000, recombinant Strep-tagged enzyme, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
membrane-bound PQQ-dependent glucose dehydrogenase | - |
Gluconobacter oxydans |
mGDH | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Gluconobacter oxydans |