Cloned (Comment) | Organism |
---|---|
into a His-tagged vector for expression in Escherichia coli JM109 | Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
A71P/N454S | mutant, relative activity vs wild type, substrate glucose 0.95, substrate maltose 0.75 | Acinetobacter calcoaceticus |
A98G/K126R/L445I/N454S | mutant, relative activity vs wild type, substrate glucose 1.00, substrate maltose 0.78 | Acinetobacter calcoaceticus |
G100R | mutant, relative activity vs wild type, substrate glucose 0.35, substrate maltose 0.26 | Acinetobacter calcoaceticus |
G100W/G320E/M367P/A376T | mutant, relative activity vs wild type, substrate glucose 0.55, substrate maltose 0.20 | Acinetobacter calcoaceticus |
G320D/M367P/A376T | mutant, relative activity vs wild type, substrate glucose 0.51, substrate maltose 0.16 | Acinetobacter calcoaceticus |
G320D/M367P/A376T | mutant, relative activity vs wild type, substrate glucose 0.69, substrate maltose 0.24 | Acinetobacter calcoaceticus |
G320E | mutant, relative activity vs wild type, substrate glucose 0.92, substrate maltose 0.70 | Acinetobacter calcoaceticus |
G320E/M367P/A376T | mutant, relative activity vs wild type, substrate glucose 0.69, substrate maltose 0.25 | Acinetobacter calcoaceticus |
G320F/M367P/A376T | mutant, relative activity vs wild type, substrate glucose 0.48, substrate maltose 0.17 | Acinetobacter calcoaceticus |
G320Y/M367P/A376T | mutant, relative activity vs wild type, substrate glucose 0.49, substrate maltose 0.16 | Acinetobacter calcoaceticus |
K3E/E278G/G392C | mutant, relative activity vs wild type, substrate glucose 0.92, substrate maltose 0.53 | Acinetobacter calcoaceticus |
L194F/A376T | mutant, relative activity vs wild type, substrate glucose 0.39, substrate maltose 0.075 | Acinetobacter calcoaceticus |
L194F/G320E/M367P | mutant, relative activity vs wild type, substrate glucose 0.38, substrate maltose 0.14 | Acinetobacter calcoaceticus |
L194F/G320E/M367P/A376T | mutant, relative activity vs wild type, substrate glucose 0.36, substrate maltose 0.051 | Acinetobacter calcoaceticus |
L194F/G320F | mutant, relative activity vs wild type, substrate glucose 0.38, substrate maltose 0.060 | Acinetobacter calcoaceticus |
L194Q | mutant, relative activity vs wild type, substrate glucose 0.22, substrate maltose 0.15 | Acinetobacter calcoaceticus |
M367P/A376T | mutant, relative activity vs wild type, substrate glucose 0.65, substrate maltose 0.24 | Acinetobacter calcoaceticus |
N454S | mutant, relative activity vs wild type, substrate glucose 0.87, substrate maltose 0.69 | Acinetobacter calcoaceticus |
Q193H | mutant, relative activity vs wild type, substrate glucose 0.41, substrate maltose 0.23 | Acinetobacter calcoaceticus |
Q193S/G320E | mutant, relative activity vs wild type, substrate glucose 0.56, substrate maltose 0.19 | Acinetobacter calcoaceticus |
V157I/M367V/T463S | mutant, relative activity vs wild type, substrate glucose 1.00, substrate maltose 0.78 | Acinetobacter calcoaceticus |
V91A/W372R | mutant, relative activity vs wild type, substrate glucose 0.44, substrate maltose 0.22 | Acinetobacter calcoaceticus |
Y248F/N342D/A376T/A418V | mutant, relative activity vs wild type, substrate glucose 0.74, substrate maltose 0.43 | Acinetobacter calcoaceticus |
Y302H | mutant, relative activity vs wild type, substrate glucose 0.47, substrate maltose 0.35 | Acinetobacter calcoaceticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
monomer | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | Acinetobacter calcoaceticus | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | P13650 | precursor | - |
Purification (Comment) | Organism |
---|---|
using a His Microspin purification module | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + ubiquinone | - |
Acinetobacter calcoaceticus | ? | - |
? | |
D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
lactose + ubiquinone | - |
Acinetobacter calcoaceticus | ? | - |
? | |
maltose + ubiquinone | - |
Acinetobacter calcoaceticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000 Da | Acinetobacter calcoaceticus |
Synonyms | Comment | Organism |
---|---|---|
PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
PQQGDH-B | - |
Acinetobacter calcoaceticus |
quinoprotein glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
activity assay | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus |