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Literature summary for 1.1.5.2 extracted from

  • Hamamatsu, N.; Suzumura, A.; Nomiya, Y.; Sato, M.; Aita, T.; Nakajima, M.; Husimi, Y.; Shibanaka, Y.
    Modified substrate specificity of pyrroloquinoline quinone glucose dehydrogenase by biased mutation assembling with optimized amino acid substitution (2006), Appl. Microbiol. Biotechnol., 73, 607-617.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into a His-tagged vector for expression in Escherichia coli JM109 Acinetobacter calcoaceticus

Protein Variants

Protein Variants Comment Organism
A71P/N454S mutant, relative activity vs wild type, substrate glucose 0.95, substrate maltose 0.75 Acinetobacter calcoaceticus
A98G/K126R/L445I/N454S mutant, relative activity vs wild type, substrate glucose 1.00, substrate maltose 0.78 Acinetobacter calcoaceticus
G100R mutant, relative activity vs wild type, substrate glucose 0.35, substrate maltose 0.26 Acinetobacter calcoaceticus
G100W/G320E/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.55, substrate maltose 0.20 Acinetobacter calcoaceticus
G320D/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.51, substrate maltose 0.16 Acinetobacter calcoaceticus
G320D/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.69, substrate maltose 0.24 Acinetobacter calcoaceticus
G320E mutant, relative activity vs wild type, substrate glucose 0.92, substrate maltose 0.70 Acinetobacter calcoaceticus
G320E/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.69, substrate maltose 0.25 Acinetobacter calcoaceticus
G320F/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.48, substrate maltose 0.17 Acinetobacter calcoaceticus
G320Y/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.49, substrate maltose 0.16 Acinetobacter calcoaceticus
K3E/E278G/G392C mutant, relative activity vs wild type, substrate glucose 0.92, substrate maltose 0.53 Acinetobacter calcoaceticus
L194F/A376T mutant, relative activity vs wild type, substrate glucose 0.39, substrate maltose 0.075 Acinetobacter calcoaceticus
L194F/G320E/M367P mutant, relative activity vs wild type, substrate glucose 0.38, substrate maltose 0.14 Acinetobacter calcoaceticus
L194F/G320E/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.36, substrate maltose 0.051 Acinetobacter calcoaceticus
L194F/G320F mutant, relative activity vs wild type, substrate glucose 0.38, substrate maltose 0.060 Acinetobacter calcoaceticus
L194Q mutant, relative activity vs wild type, substrate glucose 0.22, substrate maltose 0.15 Acinetobacter calcoaceticus
M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.65, substrate maltose 0.24 Acinetobacter calcoaceticus
N454S mutant, relative activity vs wild type, substrate glucose 0.87, substrate maltose 0.69 Acinetobacter calcoaceticus
Q193H mutant, relative activity vs wild type, substrate glucose 0.41, substrate maltose 0.23 Acinetobacter calcoaceticus
Q193S/G320E mutant, relative activity vs wild type, substrate glucose 0.56, substrate maltose 0.19 Acinetobacter calcoaceticus
V157I/M367V/T463S mutant, relative activity vs wild type, substrate glucose 1.00, substrate maltose 0.78 Acinetobacter calcoaceticus
V91A/W372R mutant, relative activity vs wild type, substrate glucose 0.44, substrate maltose 0.22 Acinetobacter calcoaceticus
Y248F/N342D/A376T/A418V mutant, relative activity vs wild type, substrate glucose 0.74, substrate maltose 0.43 Acinetobacter calcoaceticus
Y302H mutant, relative activity vs wild type, substrate glucose 0.47, substrate maltose 0.35 Acinetobacter calcoaceticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Acinetobacter calcoaceticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
monomer Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ubiquinone Acinetobacter calcoaceticus
-
D-glucono-1,5-lactone + ubiquinol
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus P13650 precursor
-

Purification (Commentary)

Purification (Comment) Organism
using a His Microspin purification module Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + ubiquinone
-
Acinetobacter calcoaceticus ?
-
?
D-glucose + ubiquinone
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
?
lactose + ubiquinone
-
Acinetobacter calcoaceticus ?
-
?
maltose + ubiquinone
-
Acinetobacter calcoaceticus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 50000 Da Acinetobacter calcoaceticus

Synonyms

Synonyms Comment Organism
PQQ glucose dehydrogenase
-
Acinetobacter calcoaceticus
PQQGDH-B
-
Acinetobacter calcoaceticus
quinoprotein glucose dehydrogenase
-
Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
activity assay Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
Acinetobacter calcoaceticus