Literature summary for 1.1.5.2 extracted from

Igarashi, S.; Ohtera, T.; Yoshida, H.; Witarto, A.B.; Sode, K.
Construction and characterization of mutant water-soluble PQQ glucose dehydrogenases with altered K(m) values--site-directed mutagenesis studies on the putative active site (1999), Biochem. Biophys. Res. Commun., 264, 820-824.

Protein Variants

Protein Variants	Comment	Organism
D276E	drastic decrease in EDTA tolerance	Acinetobacter calcoaceticus
E277A	decreased Km value for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme	Acinetobacter calcoaceticus
E277D	decreased Km value for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme	Acinetobacter calcoaceticus
E277G	drastic decrease in EDTA tolerance	Acinetobacter calcoaceticus
E277H	decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme	Acinetobacter calcoaceticus
E277K	decreased Km value for glucose and altered substrate specificity, significantly increased catalytic efficiency compared with the wild-type enzyme	Acinetobacter calcoaceticus
E277N	decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme	Acinetobacter calcoaceticus
E277Q	decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme	Acinetobacter calcoaceticus
E277V	decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme	Acinetobacter calcoaceticus
N275E	drastic decrease in EDTA tolerance	Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.3	-	D-glucose	pH 7.0, mutant enzyme E277G	Acinetobacter calcoaceticus
1.2	-	D-glucose	mutant E277N	Acinetobacter calcoaceticus
1.5	-	D-glucose	mutant enzyme E277A	Acinetobacter calcoaceticus
2.5	-	D-glucose	pH 7.0, mutant enzyme E277V	Acinetobacter calcoaceticus
4.3	-	D-glucose	pH 7.0, mutant enzyme E277Q	Acinetobacter calcoaceticus
6.8	-	D-galactose	pH 7.0, wild-type enzyme and mutant enzyme E277K	Acinetobacter calcoaceticus
7	-	D-glucose	pH 7.0, mutant enzyme I278F	Acinetobacter calcoaceticus
7.4	-	D-glucose	pH 7.0, mutant enzyme E277D	Acinetobacter calcoaceticus
7.5	-	lactose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
7.7	-	D-glucose	pH 7.0, mutant enzyme E277H	Acinetobacter calcoaceticus
8.8	-	D-glucose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
8.9	-	D-glucose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
14.3	-	maltose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
14.3	-	lactose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
14.3	-	D-xylose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
15.7	-	D-glucose	pH 7.0, mutant enzyme N279H	Acinetobacter calcoaceticus
21	-	allose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
22	-	D-mannose	pH 7.0, wild-type enzyme and mutant enzyme E277K	Acinetobacter calcoaceticus
24	-	D-glucose	pH 7.0, mutant enzyme D275E and D276E	Acinetobacter calcoaceticus
26	-	D-glucose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
27	-	3-O-methyl-D-glucose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
29	-	D-allose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
30.9	-	maltose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
34	-	D-xylose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
46	-	3-O-methyl-D-glucose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
88	-	2-deoxy-D-glucose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
90	-	2-deoxy-D-glucose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter calcoaceticus	-	expression in Escherichia coli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2-deoxy-D-glucose + pyrroloquinoline quinone	-	Acinetobacter calcoaceticus	2-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol	-	?
3-O-methyl-D-glucose + pyrroloquinoline quinone	-	Acinetobacter calcoaceticus	3-O-methyl-D-glucono-1,5-lactone + pyrroloquinoline quinol	-	?
D-allose + pyrroloquinoline quinone	-	Acinetobacter calcoaceticus	D-allono-1,5-lactone + pyrroloquinoline quinol	-	?
D-galactose + pyrroloquinoline quinone	-	Acinetobacter calcoaceticus	D-galactono-1,5-lactone + pyrroloquinoline quinol	-	?
D-glucose + 2,6-dichlorophenol-indophenol	-	Acinetobacter calcoaceticus	D-glucono-1,5-lactone + ?	-	?
D-glucose + phenazine methosulfate	-	Acinetobacter calcoaceticus	D-glucono-1,5-lactone + ?	-	?
D-mannose + pyrroloquinoline quinone	-	Acinetobacter calcoaceticus	D-mannono-1,5-lactone + pyrroloquinoline quinol	-	?
D-xylose + pyrroloquinoline quinone	-	Acinetobacter calcoaceticus	D-xylono-1,5-lactone + pyrroloquinoline quinol	-	?
lactose + pyrroloquinoline quinone	-	Acinetobacter calcoaceticus	?	-	?
maltose + pyrroloquinoline quinone	-	Acinetobacter calcoaceticus	?	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	t1/2: 10 min for wild-type enzyme and mutant enzyme E277K, 4 min for mutant enzymes E277Q and N279H, 25 min for mutant enzyme I278F, less than 2 min for mutant enzymes E277G, E277A, E277D, E277H, E277N, E277V, D275E and D276E	Acinetobacter calcoaceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
121	-	D-galactose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
201	-	D-xylose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
267	-	D-mannose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
331	-	2-deoxy-D-glucose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
630	-	D-galactose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
669	-	lactose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
678	-	D-xylose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
785	-	maltose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
861	-	D-mannose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
1020	-	maltose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
1060	-	2-deoxy-D-glucose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
1440	-	D-allose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
1450	-	3-O-methyl-D-glucose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
1800	-	lactose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
3070	-	D-glucose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
3200	-	3-O-methyl-D-glucose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus
3440	-	D-glucose	pH 7.0, wild-type enzyme	Acinetobacter calcoaceticus
4560	-	D-allose	pH 7.0, mutant enzyme E277K	Acinetobacter calcoaceticus