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Literature summary for 1.1.5.2 extracted from
- D-Glucose dehydrogenase from Pseudomonas fluorescens, membrane-bound (1982), Methods Enzymol., 89, 149-154.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 3.3 mM, complete inhibition | Pseudomonas fluorescens |  |
| p-benzoquinone | 1.7 mM, complete inhibition | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0097 | - |
ubiquinone Q6 | pH 8.8, 25°C | Pseudomonas fluorescens | |
| 0.031 | - |
ubiquinone Q4 | pH 8.8, 25°C | Pseudomonas fluorescens | |
| 0.06 | - |
ubiquinone Q1 | pH 8.8, 25°C | Pseudomonas fluorescens | |
| 0.061 | - |
ubiquinone Q2 | pH 8.8, 25°C | Pseudomonas fluorescens | |
| 0.13 | - |
phenazine methosulfate | pH 8.8, 25°C | Pseudomonas fluorescens | |
| 0.47 | - |
D-glucose | pH 6.0, 25°C, reaction with 2,6-dichlorophenolindophenol | Pseudomonas fluorescens | |
| 0.56 | - |
N,N,N,N-tetramethyl-o-phenylenediamine | pH 8.8, 25°C | Pseudomonas fluorescens | |
| 0.69 | - |
ferricyanide | pH 8.8, 25°C | Pseudomonas fluorescens | |
| 1.6 | - |
2,6-dichlorophenolindophenol | pH 8.8, 25°C | Pseudomonas fluorescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | outer surface of the cytoplasmic membrane | Pseudomonas fluorescens | - |
- |
| membrane | bound to | Pseudomonas fluorescens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
1 * 87000, urea-SDS-PAGE | Pseudomonas fluorescens |
| 93000 | - |
sucrose density gradient centrifugation | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 386 | - |
- |
Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + pyrroloquinoline quinone | 6.5% of the activity with D-glucose | Pseudomonas fluorescens | D-galactono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-glucose + ferricyanide | - |
Pseudomonas fluorescens | D-glucono-1,5-lactone + ferrocyanide | - |
? | |
| D-glucose + N,N,N,N-tetramethyl-o-phenylenediamine | - |
Pseudomonas fluorescens | ? | - |
r | |
| D-glucose + phenazine methosulfate | - |
Pseudomonas fluorescens | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q1 | - |
Pseudomonas fluorescens | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q2 | - |
Pseudomonas fluorescens | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q4 | - |
Pseudomonas fluorescens | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q6 | - |
Pseudomonas fluorescens | D-glucono-1,5-lactone + ? | - |
? | |
| D-mannose + pyrroloquinoline quinone | 8.6% of the activity with D-glucose | Pseudomonas fluorescens | D-mannono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-xylose + pyrroloquinoline quinone | 13% of the activity with D-glucose | Pseudomonas fluorescens | D-xylono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| L-arabinose + pyrroloquinoline quinone | 2.8% of the activity with D-glucose | Pseudomonas fluorescens | L-arabino-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| maltose + pyrroloquinoline quinone | 3.2% of the activity with D-glucose | Pseudomonas fluorescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 87000, urea-SDS-PAGE | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | prosthetic group | Pseudomonas fluorescens | |
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