Any feedback?
Literature summary for 1.1.5.2 extracted from
- Quinoprotein D-glucose dehydrogenase in Acinetobacetr calcoaceticus LMD 79.41: purification and characterization of the membrane-bound enzyme distinct from the soluble enzyme (1989), Antonie van Leeuwenhoek, 56, 63-72.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0034 | - |
ubiquinone Q6 | membrane-bound enzyme form | Acinetobacter calcoaceticus |  |
| 0.0044 | - |
ubiquinone Q9 | membrane-bound enzyme form | Acinetobacter calcoaceticus | |
| 0.012 | - |
ubiquinone Q2 | membrane-bound enzyme form | Acinetobacter calcoaceticus | |
| 0.0177 | - |
ubiquinone Q2 | soluble enzyme form | Acinetobacter calcoaceticus | |
| 0.074 | - |
phenazine methosulfate | membrane-bound enzyme form | Acinetobacter calcoaceticus | |
| 0.148 | - |
ubiquinone Q1 | membrane-bound enzyme form | Acinetobacter calcoaceticus | |
| 0.178 | - |
ubiquinone Q1 | soluble enzyme form | Acinetobacter calcoaceticus | |
| 1.9 | - |
phenazine methosulfate | soluble enzyme form | Acinetobacter calcoaceticus | |
| 4.2 | - |
D-glucose | membrane-bound enzyme form | Acinetobacter calcoaceticus | |
| 24.5 | - |
D-glucose | soluble enzyme | Acinetobacter calcoaceticus | |
| 26.7 | - |
lactose | soluble enzyme | Acinetobacter calcoaceticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | enzyme exists as a soluble form and a membrane-bound form | Acinetobacter calcoaceticus | 16020 | - |
| soluble | - |
Acinetobacter calcoaceticus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, soluble enzyme SDS-PAGE | Acinetobacter calcoaceticus |
| 83000 | - |
1 * 83000, membrane-bound enzyme form, mainly monomeric in detergent solution | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + pyrroloquinoline quinone | Acinetobacter calcoaceticus | membrane-bound enzyme functions by linking to the respiratory chain via ubiquinone | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-glucose + pyrroloquinoline quinone | Acinetobacter calcoaceticus LMD 79.41 | membrane-bound enzyme functions by linking to the respiratory chain via ubiquinone | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
LMD 79.41 | - |
| Acinetobacter calcoaceticus LMD 79.41 | - |
LMD 79.41 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 570 | - |
membrane bound enzyme form | Acinetobacter calcoaceticus |
| 2209 | - |
soluble enzyme form | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + phenazine methosulfate | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + phenazine methosulfate | - |
Acinetobacter calcoaceticus LMD 79.41 | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + pyrroloquinoline quinone | membrane-bound enzyme functions by linking to the respiratory chain via ubiquinone | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-glucose + pyrroloquinoline quinone | membrane-bound enzyme functions by linking to the respiratory chain via ubiquinone | Acinetobacter calcoaceticus LMD 79.41 | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-glucose + ubiquinone Q1 | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q1 | - |
Acinetobacter calcoaceticus LMD 79.41 | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q2 | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q2 | - |
Acinetobacter calcoaceticus LMD 79.41 | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q6 | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q6 | - |
Acinetobacter calcoaceticus LMD 79.41 | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone Q9 | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol Q9 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, soluble enzyme SDS-PAGE | Acinetobacter calcoaceticus |
| monomer | 1 * 83000, membrane-bound enzyme form, mainly monomeric in detergent solution | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
soluble enzyme | Acinetobacter calcoaceticus |
| 8.5 | - |
membrane-bound enzyme | Acinetobacter calcoaceticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | pH 7.5: about 40% of maximal activity, pH 9.5: about 85% of maximal activity, membrane-bound enzyme, reaction with phenazine methosulfate, 2,6-dichlorophenol indophenol and pyrroloquinoline quinone | Acinetobacter calcoaceticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
